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DISINFECTION , DECONTAMINATION
AND STERILIZATION
Disinfection , decontamination and
sterilization
Learning objectives
1. Concentration of agent
microbial population.
2. Time of exposure
4. Temperature
Bactericidal potency of the chemical agent increases
with an increase in temperature.
An increase in 100cthe bacterial death rate.
5. Nature of the organism
Species of the bacteria
blood or
A. Cationic agents
• Quaternary ammonium compounds (Quates)
• Denatures protein.
• Acids like benzoic acid, citric acid and acetic acid are
helpful as food preservatives: extending storage life of
food products.
3 Chemical agents that modify functional groups of
proteins and nucleic acids
3.1 Heavy metals
• Various metals and metal salts are commonly employed
to prevent microbial growth or kill microbes
• Mercury compounds
e.g. mercuric chloride- limited use because of its toxicity.
• - It can be used as antiseptics.
e.g. Merthiolate
• Silver compounds
- Hydrogen peroxide
Uses:
• Hypochlorite: sanitizing dairy and food processing
industries, households and hospitals.
• Organic or inorganic chloramines: Is effective water
disinfectant acting by liberating chlorine.
• Iodine: effective skin disinfectant.
Example:
• Malachite green
• Brilliant green
Uses:
• Highly selective for Gram-positive bacteria.
A. Formaldehyde
Uses:
• Preservation of fresh tissues.
C. Ethylene oxide
• is gaseous sterilizing chemical.
Use:
• Used to sterilize medical-surgical devices that would be
damaged by heat.
II. Physical means of sterilization and disinfection
• Physical means of sterilization and disinfection include
Heat, Filtration and Radiation
Mechanism of Action:
• Dry heat-denatures protein.
A. Incineration
temperature.
B. Flaming
Scalpels , neck of flasks, bottles and tubes are exposed for a few
seconds, but it is of uncertain efficacy.
C. Red heat
Use:
Maximum temperature is 100 0C and will there for, not kill all
would be killed.
Uses:
• Pasteurization of milk.
will be formed which penetrates and gives up its latent heat when
it condenses on objects.
• Hot saturated stem in autoclaving acts as an excellent agent for
sterilization because of:
- High temperature;
A. Freezing
drying.
Uses:
• Preservation of microbial cultures.
• Preservation of vaccines
C. Filtration
• Liquids and gases can be sterilized by passing them
through filters. Filters that have pores smaller than the
size of the microbes retain micro-organisms.
• The filter acts as a strainer, a microbial sieve. Standard
bacteriological membrane filters are composed of
nitrocellulose and have pore diameters of 0.45 µm,
small enough to prevent passage of most bacteria.
Uses :
Preparing heat-labile culture media components
and even visible light are all forms of radiation. When these rays