Characterization of Adipose-

Derived Stem Cells (ASCs)

Student: Mary Beth
Timeline: Summer 2018
Learning Goals and Objectives
• Read the relevant literature to the project
• Observe and note the techniques and methodology applied in the lab
• Perform own experiments and record data
• Presentation of the research work
• Write manuscript based on this study
 Project layout
• Overall objective: Characterization of Adipose-Derived Stem Cells
isolated from porcine and human sources
• Techniques: Basic cell culture, flow cytometry, staining, microscopy,
XTT
Culture Multilineage
and
Observe Chondrogen
Differentiatio
Expansion proliferati n: esis ASCs vs
chondrocyte:
of porcine on (XTT) Adipogenesis,
osteogenesis RNA
and human and and isolation and
ASCs populatio chondrogene qPCR (ACAN,
(Microscop n sis (Staining COL2A1 and
y and flow and SOX9)
cytometry)
doubling microscopy)
Data
Flow Cytometry
Marker Type BD Cat. No. Format
CD90 Mouse Anti-Human 555596 PE
CD105 Mouse Anti-Human 562408 APC
CD73 Mouse Anti-Human 561258 PE-Cy™7
HLA-DR Mouse Anti-Human Dr. Brand Alexa Fluor®488
CD34 Mouse Anti-Human 562383 PE-CF594
CD45 Mouse Anti-Human 564105 PerCP-Cy™5.5
pASCs

1.05 ± 0.10 98.50 ± 0.51 0.66 ± 0.07

3.56 ± 0.22 0.15 ± 0.04 1.46 ± 0.10

hASCs

73.73 ± 0.15 82.23 ± 0.20 81.60 ± 7.45

0.34 ± 0.04 1.84 ± 0.24 0.01 ± 0.01

HP032018

81.76 ± 0.66 90.5 ± 0.20 65.56 ± 0.68

0.00 ± 0.00 1.39 ± 0.09 0.006 ± 0.005