CULTURE MEDIA ASSIGNMENT

SUBMITTED BY: MAHAM IMTIAZ

BDS-FA19-2005
 ASSIGNMENT QUESTIONS

1. Define culture media, classify the culture media On both basis, oxygen
requirement and on the basis of consistency
2. Define simple and differential media and give some examples
3. Define enriched and selective media + composition + examples
 CULTURE MEDIA

 An artificial preparation which contains all the

essential elements and nutrients needed by
the microorganisms to grow.
Characteristics:
 Must give satisfactory and rapid growth from
single inoculum.
 Should be reasonably cheap and easily
reproducible
 Should enable to demonstrate all
characteristics in which we are intrested.
CLASSIFICATION OF CULTURE
MEDIA
ON THE BASIS OF CLASSIFICATION
1. CONSISTENCY 1) Broth
2) Semi solid
3) solid
II. OXYGEN A) AEROBIC
REQUIREMENT 1) Simple media
2) Enriched media
3) Differential media
4) Selective media
5) Enrichment media

B) ANEROBIC
 SIMPLE MEDIA

 It is the type of media which are used to grow microorganisms that do not
have special nutritional requirements. It fulfills basic requirement of bacteria.
 Also called basal medium.

 EXAMPLES
1. Nutrient broth
2. nutrient media.

 Uses
- Preparation of enriched media.
- To maintain stock cultures of control strains of bacteria.
- For subculturing pathogens.
 DIFFERENTIAL MEDIA

 It is the type of medium which has substance incorporated in it enabling

it to bring out different characteristics of bacteria and thus helping to
distinguish between them.
 EXAMPLES:
1. Blood agar differentiate al pha hemolysis and beta hemolysis. It is
due to the production of hemolysin by the bacteria.
2. MacConkey’s agar differentiate lactose fermenter and non-lactose
fermenter.
 ENRICHED MEDIA
 Certain organisms can’t grow on the ordinary media . They require
growth promoting ingredients such as blood, serum, glucose, egg,
Vitamins, yeast extract etc. The media containing ingredients
enhance their growth.
 EXAMPLES:
1. Blood agar
Composition:
- Melted agar
- Sterile defibrinated blood: 5-10 %
- Beef extract
- Peptone water
- NaCl

2. Chocolate agar.
Composition same as blood agar
 SELECTIVE MEDIA
These are the culture media which allows the growth of one type
of bacteria and inhibit the growth of another type of bacteria e.g.
1. MacConkeys media
-It allows the growth of G-ve bacteria and inhibit growth of G+ve bacteria
2. CLED
-It is used for the urinary tract pathogens / used for the microorganisms of UTI
- Urine sample is cultured only on CLED
- Composition:
Agar, Beef extract, Bromothymol blue, Casein enzymatic hydrosylate, L-
cystine, Lactose, Peptic digest of a normal tissue
3- L J media
- Egg based media
- Used for the mycobacterium
- Sputum specimen used for the L J medium
- 4-6 wks take by M.T.B to grow (slow growing )