WELCOME

TO
MY
PRESENTATION
 INFECTIOUS BURSAL DISEASES (IBD) IN
SONALI CHICKEN AT JOYPURHAT DISTRICT
OF BANGLADESH

DR. MOHAMMED HABIBULLAH BELALY

REGISTRATION NO. 1805141
SEMESTER: JULY-DECEMBER, 2019
SESSION: 2018-2019

MS IN MEDICINE

DEPARTMENT OF MEDICINE, SURGERY AND

OBSTETRICS
HAJEE MOHAMMAD DANESH SCIENCE AND
TECHNOLOGY UNIVERSITY, DINAJPUR
 SUPERVISORY COMMITTEE
Supervisor Co-Supervisor
Dr. Md. Shajedur Rahman Dr. Mst. Sogra Banu Juli

Professor Dr. Begum Fatema Zohara

Chairman
Defense Committee
&
Department of Medicine, Surgery
and Obstetrics

Hajee Mohammad Danesh Science and

Technology University
Dinajpur–5200.
 BACKGROUND
 Poultry industry plays a vital role in the rural socio-economic
system by contributing significantly on economic growth and
simultaneously creating numerous employment opportunities
and fulfilling the protein demand of human .
 Poultry diseases are the major constraints for developing the
poultry industry
 One of the important viral disease is infectious bursal disease
(IBD)
 Infectious bursal disease is an acute, highly contagious,
immunosuppressive and economically important poultry disease
caused by Birnaviridae RNA virus.
 BACKGROUND

 Several factors like vaccination status, biosecurity measure,

management practice and climatic condition may play an
important role in the prevalence of IBD.
 Knowledge on infectious bursal disease epidemiology is
necessary for to implement a successful prevention-control
program at farm and farmer level.
 OBJECTIVES

 To study the prevalence and mortality of infectious

bursal disease (IBD) in Sonali chicken at Joypurhat
district of Bangladesh.

 To identify age, sex, season and management- wise

prevalence and mortality of infectious bursal disease in
Sonali chicken.
 To observed the effects of vaccination on prevalence
and mortality of sonali chicken.

 To detect effectiveness of different treatment regimes

in control of IBD.
METERIALS
AND
METHODS
Study Area
The study was carried out in five
upazila (Sadar, Pancbibi,
Akkelpur, Kalai and Khetlal) of
Joypurhat district of
Bangladesh. The clinical and
postmortem examinations were
carried out for diagnosis of
infectious bursal disease at farm
and Field Disease Investigation
Laboratory(FDIL), Joypurhat.
STUDY PERIOD
The study was carried out for a period of 12
months from March 2019 to March 2020.
EXPERIMENTAL CHICKEN:
The chickens of different 21 large and small scale
commercial sonali poultry farms about 33735
birds were considered as experimental chickens.
 Study design
Selection of the study area
 Identify the target groups or population

 
Epidemiological study (Questionnaire)
 

Clinical History Clinical Examination Disease history

    Pre-disposing factor
Onset and duration of illness
Flock data
Age, Sex, Vaccination,
Biosecurity, Season
 
  Clinical examination,
Postmortem examination, Data
Analysis & Disease Diagnosis
Clinical examination
a. History
b. General examination
c. Postmortem examination

Equipment and Appliances for Necropsy

1. Bird
2. Scissors
3. Forceps
4. Goves
5. Musk
6. Scalpel
7. Chisel
8. 10% neutral buffered formalin
 STATISTICAL ANALYSIS
The collected data was analyzed by a statistical software
namely, SPSS version 24.0. Prevalence was determined by
the formula described by Thrusfield (2005) as the rate of
number of infested animals and total number of animals in
the population. Associations between explanatory
variables (age, sex, breed and season) and prevalence
were done by chi-square test. In all analysis, 95%
confidence intervals and p<0.05 were set to indicate
significance.
 PREVALENCE

Prevalence were calculated as number of cases of disease

divided by population at risk and multiple by 100.

No of cases of disease
Prevalence rate (%) =  100
Population at risk

No of dead birds
Morality (%) =  100
Affected Population
RESULTS
Table 1: OVERALL PREVALENCE AND MORTALITY
Upazila No. of Total Affected Dead Prevalenc Mortalit
Name Farm Birds Birds Birds e (%) y (%)
Visited P-value P-value

Sadar 5 6032 775 242 12.85 4.01

Pachbibi 3 4562 639 143 14.00 3.13
Akkelpu 7 13080 2629 547 20.09 4.18
<0.05 <0.05
r

Kalai 4 5021 531 197 10.54 3.92

Khetlal 2 5040 2198 846 43.61 16.78
Total 21 33735 6772 1975 19.48   5.85  

P<0.05 means statistically significant at 5% level of significance.

Graph 1: Morbidity and mortality of birds for IBD
according to Upazila

45
40
35
30
25
Morbidity
20
Mortality
15
10
5
0
Sadar Pancbibi Akkelpur Kalai Khetlal Total
Graph 2: Total birds’ wise distribution of infectious
bursal disease in Joypurhat district

40000
30000
20000
10000
Number
0
Total Birds Total affected Total Dead
Table 2: AGE- WISE PREVALENCE AND
MORTALITY OF IBD IN SONALI CHICKEN
Age Total Affected Dead Prevalen P-value Morbidit P-value
group Bird Bird Bird ce y

3 to 4 22130 4797 1278 21.68 5.77

week

5 to 6 10079 1789 652 17.75 6.47

week <0.05 <0.05

7 to 8 1526 186 45 12.19 2.95

week

Total 33735 6772 1975 19.48   5.85  

Graph 3: Prevalence of IBD according to age

70
60
50
40 Number of Farms
30 Prevalence
20 Mortality

10
0
3-4 weeks 5-6 weeks 7-8weeks
Table 3: SEX WISE PREVALENCE AND MORTALITY
Sex Number Affecte Dead Prevalen P- Mortality P-
Group of Birds d Birds Birds ce (%) value (%) value

Male 13595 2537 584 18.66 4.30

<0.05 <0.05
Female 20140 4035 1391 20.03 6.91

Total 33735 6572 1975 19.48   5.85  

Graph 4: Prevalence of IBD according to sex of birds

25

20

15 Male
10 Female

0
Morbidity Mortality
 Table 4 : SEASON WISE PREVALENCE AND MORTALITY
   
Season Farm Affected Prevalence Dead Birds Mortality  
Number Birds (%) P- (%)
value P-
value

Winter 08 15,570 46.15 795 5.11

(Dec-Feb)

Summer
04 5,630 16.78 319 5.66
(Mar-May)
<0.0
Rainy <0.05
03 4,350 12.89 5 204 4.69
(Jun-Aug)

Autumn
(Sep-Nov) 05 8,185 24.26 657 8.02

Total 21 33,735     1975 5.85  

Table 5: VACCINATION - WISE PREVALENCE AND
MORTALITY
Vaccine Name Number Number Affected Dead Prevalenc Mortality
of Farm of Birds Birds No. Bird No. e (%)
(%)

GumboMed Plus 3 3075 589 113 19.15 3.67

(Incepta)
 
Cevac IBD (ACI) 2 2570 846 376 32.92 14.63
AviPro® IB Ark 5 8570 324 76 3.78 0.89
(Elanco)

BAU 404 (Govt) 3 5214 1559 478 23.9 9.16

Izovac Gumboro 8 14306 3254 923 22.75 6.45
(Renata)

Total 21 33735 6572 1975 19.48 5.85

P-value         <0.05 <0.05
 Table 6: FARM CONSTRUCTION-WISE PREVALENCE AND
MORTALITY
Floor type Number Number Affected Dead Bird Prevalence Mortality
of Farm of Birds Birds No. No. (%) (%)

Concrete 4 8597 389 215 2.5 3.52

Kacha 17 25138 6183 1760 7.00 24.60

Total 21 33735 6572 1975 5.85 19.48

P-value         <0.05 <0.05

 Table 7: BIOSECURITY WISE-EPREVALENCE AND
MORTALITY
Fumigation Number Number Affected Dead Bird No. Prevalence Mortality
of Farm of Birds Birds No. (%) (%)

Fumigated 4 9512 742 155 7.80 1.63

5830
Not 17 24223 1820 24.07 7.51
Fumigated

Total 21 33735 6572 1975 19.48 5.85

P-value         <0.05 <0.05

Table 8: TREATMENT STATUS
Name No. of Name of antibiotic & Dose & route of Range of P-Value
of the Birds others Medicine administration complete
group treated recovery
days

Group- 300 a)Livoofloxacine(10%) a) 1ml/1L Drinking 4±0.75

C b)Vitamin-C (Ascorbic water 16hrs daily for 5
Acid-98%) days.
c)Ph(Formic acid,Lactic b)1gm/3L drinking
acid,Propionic acid & water 6hrs daily for <0.05
HMTBa) 3days
d)Mollasses 5% c)1ml/3L drinking
e)Andopan water 6hrs daily for 5
days
d)20gm/L drinking
water 8hrs for 3 days
e)1gm/25kg body wt.
with drinking water
8hrs daily for 5 days
Table 8: TREATMENT STATUS
Name No. of Name of antibiotic & Dose & route of Range of P-Value
of the Birds others Medicine administration complet
group treated e
recovery
days

Group- 300 a)Levofloxacine(10%) a) 1ml/1L Drinking 7±0.25

B b)Vitamin-C (Ascorbic water 16hrs daily for 5  
Acid-98%) days.
c)Ph(Formic acid,Lactic b)1gm/3L drinking
acid,Propionic acid & water 6hrs daily for <0.05
HMTBa) 3days
d)Mollasses 5% c)1ml/3L drinking
water 6hrs daily for 5
days
d)20gm/L drinking
water 8hrs for 3 days
Table 8: TREATMENT STATUS
Name of No. of Name of antibiotic & Dose & route of administration Range of P-Value
the Birds others Medicine complete
group treated recovery
days

Group-D 300 a)Florfenicol(20%) a) 1ml/1L Drinking water 16hrs daily 8±0.25

b)Vitamin-C (Ascorbic for 5 days.
Acid-98%) b)1gm/3L drinking water 6hrs daily
c)Ph(Formic acid,Lactic for 3days
acid,Propionic acid & c)1ml/3L drinking water 6hrs daily for <0.05
HMTBa) 5 days
d)Mollasses 5% d)20gm/L drinking water 8hrs for 3
e)Andopan days
  e)1gm/25kg body wt. with drinking
water 8hrs daily for 5 days
Table 8: TREATMENT STATUS
Name of No. of Name of antibiotic & Dose & route of administration Range of P-Value
the Birds others Medicine complete
group treated recovery
days

Group-A 300 a)Ciprofloxacine(10%) a) 1ml/1L Drinking water 16hrs 10±0.5

b)Vitamin-C (Ascorbic daily for 5 days.  
Acid-98%) b)1gm/3L drinking water 6hrs
c)Ph(Formic daily for 3days
acid,Lactic c)1ml/3L drinking water 6hrs daily <0.05
acid,Propionic acid & for 5days
HMTBa)

P<0.05 means statistically significant at 5% level of significance.

Treatment group C was most effective than others

 CONCLUSION

 Most prevalence in Khetlal lowest in Kalai, Highst Mortality

Khetlal lowest in Panchbibi Upazila in Joypurhat
 It was found that 3 to 4 weeks’ age group of chickens were more
susceptible to diseases.
 Male are less susceptible then female birds.
 Most prevalence in Winter and lowest in Rainy season and highest
mortality in Autumn season .
 AviPro® IB Ark (Elanco) was more protective than others.
 Kacha and Not fumigated farms are more susceptible to disease the
fumigated and concrete floor.
 Treatment group C was most effective than others ( B>D>A)
 Figure 2: Postmortem Examination
IBD affected Farm visit Joypurhat Sadar
Figure-3: Inspection of an IBD
infected Farm
(A) normal bursa-no edematous swelling and hemorrhage,
(B) swollen, hemorrhagic and edematous bursa.
(C, D) swollen, hemorrhagic and edematous bursa.
Hemorrhage in the thigh and pectoral muscles
(Figure-6 E, F)
Hemorrhages in the junction of proventiculus and
gizzard ( G, H)
Thanks To All