ROLE OF LEDs IN INDUCTION OF IN VITRO

FLOWERING VIA PLANT TISSUE CULTURE

TECHNOLOGY
GROUP MEMBERS:

Siti Sarah binti Tajudin (134485) │Nurin Aisyah binti Razak (134687) │Nurulhuda Athirah binti Hadri (134693) │
Aini Mastura binti Nasruddeen (134614) │Nurul Izzati binti Mohamad Rodi (135631)│Nurhazirah Syazwani binti
Zawawi (134686)
 Introduction
In vitro Flowering

Technique to develop flowers under an aseptic Light-emitting diodes (LEDs) as induction

conditions. The shoot or nodal segment of plant are
cultured in a controlled environment and nutrition.
This process involves the switch from vegetative stage to
reproductive stage of growth, regulated by both internal
and external factors.
Under LED illumination, variety of desired
morphological and physiological changes in plants
have been observed, for example, increasing
photosynthesis modulating plant morphogenesis
and induction of flowering .

Application of cytokinins, sucrose concentrations,

photoperiod, and subculture duration is very important to
promote in vitro flowering.

Convenient tool to study specific aspects of flowering,

floral initiation, floral organ development, and floral
senescence.
Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
Factors that influence in vitro plant growth and development

Light intensity
Photosynthesis, photochemical
reaction within chloroplast
Nutritional factors
Plant growth regulator Light quality (spectral
distribution)
Red, blue, green, orange red color
Temperature give effect on photomorphogenesis

Light
Light duration (photoperiod)
For flowering

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 Effect of different light colors/wavelength
Green (495nm to 570nm) Blue (450 nm to 495nm)
• Enhanced production of chlorophyll • Improves plant’s growth and maturity
• Useful in commercial greenhouses rates
for customer purchases • Influences formation of chlorophyll
which enables the more energy uptake
from the sun
Red (620nm to 750nm)
• Red colors helps flowers to bloom Yellow (570nm to 590nm)
• Combination of red and blue colors • Similar effect as green light on plant growth
is beneficial for plant maturation and but it doesn’t directly help to produce
gives high number of leaves chlorophyll
• Far-red light produce greater yields. • Removal of yellow light produce healthier
plants compared to plant naturally growing
Violet (380nm to 445nm) outside(full-spectrum of light)
• Color, taste and aroma of the plants
are enhanced Ultraviolet (20nm to 380nm)
• Increases antioxidant level in plants • Plants raised without exposure to UV
light enhanced plants growth

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 Type of light source

ARTIFICIAL

Fluorescent light
NATURAL
Incandescent light
Sunlight
Metal halide lamp

High pressure sodium lamp

Light emitting diode (LED)

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 Type of light source
WHITE FLUORESCENT LIGHT LIGHT EMITTING DIODE (LED)

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 Type of light source

FLUORESCENT WHITE LIGHT LIGHT EMITTING DIODE (LED)

Low-pressure mercury-vapor gas lamp that Two-lead semiconductor light source

uses fluorescence to create visible light

Emit photons over a broad spectral range Emit nearly monochromatic lights

10000 hours 30000 hours

consumes a lot of electrical power Use less energy

generating heat relatively cool emitting surface

Low initial investment High initial investment

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 Advantages of LED
Why LED is more preferred?

● Less heat radiation

● Monochromatic spectrum
● Greater durability
● Low power consumption

Advantages LED for in vitro flowering

● Promote plant growth

● Reduce the time a plant need to flower *All the advantages
● Produce large leaves depend on the colour of
● Regulate flowering, dormancy period, and seed germination LED used.
● Increase chlorophyll content
● Increase the photosynthetic rate and etc.

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 Application of LED

METHODOLOGY

Light treatment

MS30 Basal medium

medium

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 Application of LED
RESULT

Shoot multiplication
Leaves per shoot
rate

Shoot height Fresh and dry weight

Phenolic acid and Photosynthetic

flavonoids pigment content

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 Conclusion

LED has been

demonstrated to be LED lighting Improvement on all
an artificial flexible LEDs have been system can also types of lighting
lighting source for proved to reduce provide precision technologies will
in vitro plant tissue the electricity cost delivery of photons reduce cost per
culture as it appear and the investment and that they can photon and
to be the only light (high capital cost) be a more cost- improve reliability
source which can will be returned as effective option for of LED in plant
provide true profit in long-term supplemental tissue culture
flexibility of operations. greenhouse systems.
controlling spectral lighting.
composition.

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology
 References
Bello‐Bello, J. J., Pérez‐Sato, J. A., Cruz‐Cruz, C. A., & Martínez ‐Estrada, E. (2017). Light ‐Emitting Diodes: Progress in Plant
Micropropagation. In Chlorophyll. https://doi.org/10.5772/67913

Garden Myth. Retrieved from LED Grow Lights – Getting the Right Color Spectrum:
https://www.gardenmyths.com/led-grow-lights-color-spectrum/

Cioć, M., Szewczyk, A., Żupnik, M., Kalisz, A., & Pawłowska, B. (2018). LED lighting affects plant growth, morphogenesis and
phytochemical contents of Myrtus communis L. in vitro. Plant Cell, Tissue and Organ Culture, 132(3), 433–447.
https://doi.org/10.1007/s11240-017-1340-2

Gupta, S. D., & Jatothu, B. (2013). Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and
morphogenesis. Plant Biotechnology Reports, 7(3), 211-220.DOI 10.1007/s11816-013-0277-0

Does The Color Of Light Affect Plant Growth – Greenhouse Today. (n.d.). Retrieved July 10, 2020, from
https://www.greenhousetoday.com/does-the-color-of-light-affect-plant-growth/.

Dickens, C. W. S., & Staden, J. VAN. (1988). The In Vitro Flowering of Kalanchöe blossfeldiana Poellniz. Journal of Experimental
Botany, 39(4), 461–471. https://doi.org/10.1093/jxb/39.4.461

Role Of LEDs In Induction Of In Vitro Flowering Via Plant Tissue Culture Technology