Professional Documents
Culture Documents
Specimen: Fresh stool was collected and sent in a transport container to the UCLA clinical microbiology
lab for culture.
MacConkey agar –
• Contains bile salts and crystal violet, which inhibit the
growth of most Gram-positive bacteria. Bacteria that
ferment lactose appear pink on this agar.
Latex reagent 1:
• Green latex (anti-Salmonella grp. D1 Abs).
Latex reagent 2:
• Blue latex (anti-Salmonella grps. E & G Abs).
5. Los Angeles County Public Health Laboratory determined the isolate as a Salmonella enterica
subspecies enterica serotype Heidelberg (part of a larger US outbreak).
UCLA Patient
•Before culture completion, the patient was discharged without antibiotics for presumed viral enteritis.
•Given the patient’s age and complicated medical history, antibiotics were warranted to ensure full
recovery. Antibiotics were prescribed and the patient improved.
6 subspecies of S. enterica.
• >2500 serotypes of S. enterica subsp. Enterica.
Classification - Salmonella species
and serotypes
• Oral or intravenous rehydration.
• Generally antibiotics are not recommended.
– Agents do not shorten course of illness.
– Antibiotic resistance increasing problem.
– Antibiotics can prolong excretion of Salmonella.
– Exceptions include high risk groups.
• Underlying disease or complicated medical history
• Young children
• Disseminated infection
• Antibiotics
– Objective is to treat or prevent recurrence of bacteremic disease .
– Fluoroquinolones
– Azithromycin
– TMP-SMX
Ian McHardy, Ph.D.