Professional Documents
Culture Documents
prepared by:
Getenet.d
07/10/2022 getenet d 1
The Chain of Infection
Infectious agent or pathogen
Reservoir
Portal of exit
Mode of transmission
Portal of entry
Susceptible host
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ASEPSIS
• The term asepsis means the absence of disease-
producing microorganisms.
• Infection:- is the invasion of the body by micro
organism
• Infection can be symptomatic or asymptomatic
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Concept of Asepsis
nursing procedures.
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Types of Asepsis Technique
• There are two types of asepsis:
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Surgical or Sterile Asepsis or Sterile
Technique
• includes procedures used to eliminate micro-
organisms from an area & is practiced by nurses in
ORTs, labour & delivery area, major diagnostic
areas & Rx areas.
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Medical Asepsis
• During daily routine care, the nurse uses basic
medical aseptic techniques to break the infection
chain.
• E .g of medical asepsis are changing client’s bed
linen daily, handwashing, barrier techniques, &
routine environmental cleaning.
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1. Hand Hygiene
Proper hand hygiene and the use of protective glove is
a key component in minimizing the transmission of
disease causing microorganisms and maintaining an
infection-free environment.
Appropriate hand hygiene must be carried out:
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cont….d
oWater
o07/10/2022
friction getenet d 10
Hand washing procedure
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Hand washing procedure
• Wash for 20 seconds, or as long as it
takes to sing “Happy Birthday”
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Handwashing procedure
Dry hands using a paper towel or
electric hand dryer
Use paper towels to turn off the tap
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Hand Hygiene: Frequently Missed Areas
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For appropriate hand rub:
o Apply enough alcohol-based antiseptic to cover
the entire surface of hands and fingers.
o Rub the solutions vigorously into hands,
especially between the fingers and under the
nails until dry.
o Do not rinse hands after applying hand rub
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Efficacy of Hand Hygiene Preparations in Killing
Bacteria in Health Care Settings
GOOD BETTER BEST
PLAIN
SOAP & ANTIMICROBIAL ALCOHOL-
WATER SOAP & WATER BASED HAND
RUB*
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Soap and water vs. hand sanitizers
Soap and water Hand sanitizer(alcohol
based hand rub)
Does not kill anything, but Kills all germs- the good, the
washes it away bad and the harmless
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Cont….d
Gloves
• Gloves are a form of personal protective equipment.
3.Utility gloves
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Cont…..d
• Change gloves
Mask or respirator
• Gown
• Mask or respirator
Gowning
• The purpose of wearing sterile gown is in order to
prevent your self from infectious and non infectious
fluids
• There are two methods gowning:-
Gowning self and
Gowning another
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Surgical Asepsis
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Cont…
• These techniques can be practiced by nurses in the
OR (surgical incision) or at the bedside (e.g.,
inserting IV or urinary catheter & reapplying
sterile dressings) where sterile instruments &
supplies are used.
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Cont…
• In surgical asepsis, an area or object may be
considered contaminated if touched by an object
that is not sterile (e.g., a tear in a surgical glove
during a procedure, a sterile instrument placed
on an unsterile surface).
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Cont…d
• The nurse working with a sterile field or with
sterile equipment must understand that the
slightest break in technique results in
contamination.
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• How to put on Sterile Gloves
Wearing sterile gloves is part of aseptic hand hygiene, since the hands
• The second glove should then be touched only by the other sterile glove.
Step 1:Prepare a large, clean, dry area for opening the package of gloves.
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Cont…….d
• Step 2
Open the inner glove wrapper, exposing the cuffed gloves with the palms up.
• Step 3
Pick up the first glove by the cuff, touching only the inside portion of the cuff
(the inside is the side that will be touching your skin when the glove is on).
• Step 4
While holding the cuff in one hand, slip your other hand into the glove.
(Pointing the fingers of the glove toward the floor will keep the fingers open)
.Be careful not to touch anything, and hold the gloves above your waist level.
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• NOTE: If the first glove is not fitted correctly, wait to make any
adjustment until the second glove is on. Then use the sterile
fingers of one glove to adjust the sterile portion of the other glove.
Step 5
Pick up the second glove by sliding the fingers of the gloved hand
the gloved hand with the ungloved hand as the second glove is
• Step 6
• It 07/10/2022
is important to ensure that gloves fit correctly.
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• Gloves should not be washed, or decontaminated
care setting.
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Definition of terms
Sterilization
A physical or chemical process that completely destroys or removes
all microbial life, including spores.
Disinfection
It is killing or removing of harmful microorganisms
Disinfectant
Products used to kill microorganisms on inanimate objects or surfaces.
Disinfectants are not necessarily sporicidal, but may be sporostatic,
inhibiting germination or outgrowth
Antiseptic
A product that destroys or inhibits the growth of microorganisms in or
on living
The level of disinfection achieved depends on
several factors:
• contact time
• temperature
• type and concentration of the active ingredients of
the chemical germicide
• the nature of the microbial contamination.
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Types of disinfection
• High-level disinfection: can be expected to destroy all
microorganisms, with the exception of large numbers of
bacterial spores.
semicritical items
noncritical items
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Critical items
Critical items are instruments or objects that are introduced
directly into the bloodstream or into other normally sterile
areas of the body.
Examples of critical items are surgical instruments,
cardiac catheters, implants, and the blood compartment of a
hemodialyzer.
Sterility at the time of use is required for these items;.
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Semicritical items
• These items come in contact with intact mucous
membranes, but they do not ordinarily penetrate body
surfaces.
Examples are noninvasive flexible and rigid fiberoptic
endoscopes, endotracheal tubes, anesthesia breathing
circuits, and cystoscopes.
Sterilization is not absolutely essential; at a minimum, a
high-level disinfection is recommended.
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Noncritical items
• Noncritical items are those that either do not
ordinarily touch the patient or touch only intact skin.
• Such items include crutches, bedboards, blood
pressure cuffs, and a variety of other medical
accessories.
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For noncritical items
cleaning can consist only of
2) rinsing,
3) thorough drying.
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Processes for sterilization
• Dray
• Enter into autoclave
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Methods of Sterilization
1. Physical methods
Heat
Dry
Moist
Radiation
U.V. light
Ionizing radiation
Filtration
2. Chemical Methods
Radiation:
– U.V. light- Has limited sterilizing power because of poor
penetration into most materials. Generally used in
irradiation of air in certain areas e.g.. Operating Rooms
and T.B. laboratories.
– Ionizing radiation- e.g. Gamma radiation: Source
Cobalt60 has greater energy than U.V. light, therefore
more effective. Used mainly in industrial facilities e.g.
sterilization of disposable plastic syringes, gloves,
specimens containers.
Filtration
Best known example is the membrane filter
made from cellulose acetate. Generally
removes most bacteria but viruses and some
small bacteria e.g. Chlamydia & Mycoplasmas
may pass through. Thus filtration does not
technically sterilize items
Main use: for heat labile substances e.g.
antibiotics.
The recommended size filter that will
exclude the smallest bacterial cells is 0.22
micron
Sterilization by Heat: Most common
method
Dry Heat
Simplest method is exposing the item to be
sterilized to the naked flame.
Hot air oven expose items to 160°C for 1
hour.
It has electric element in the chamber as
source of heat plus a fan to circulate air for
even distribution of heat in chamber.
Used for Metals, Glassware
Moist Heat: Uses hot water. Moist heat kills
microorganisms by denaturing proteins.
Range of Action : Disinfectants are not equally effective against the whole
spectrum of microbes. e.g. Chlorhexidine is less active against GNB than Gram
Positive Cocci.
Iodine is effective against all kinds of bacteria, many endospores, fungi, and some
viruses. Its mechanism of activity may be its combination with the amino acid
used for disinfecting municipal water supplies, swimming pools, and sewage.
Chloramines consist of chlorine and ammonia. They are more stable than most
Chlorine dioxide in gaseous form is used for area disinfection, most notably to
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PARACENTHESIS
• Objectives
– At the end of this lesson the students will be able
to:-
• Define the term abdominal paracenthesis.
• Describe the term ascites.
• List the common indications of abdominal
paracenthesis.
• Discuss the procedure of abdominal
paracenthesis.
• List and describe the contraindications and
complications of the procedure.
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Anatomy of Abdominal cavity …revision
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Anatomy of Abdominal cavity …revision
– Visceral peritoneum
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PARACENTESIS
• Definition
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PARACENTESIS
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Cont……d
• Indications
– To relieve abdominal pressure from ascites
– To diagnose spontaneous bacterial peritonitis and
other infections (e.g. Abdominal TB)
– To diagnose metastatic cancer
– To diagnose blood in peritoneal space in trauma
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PARACENTESIS
• Ascites:Ascites is a medical term simply means
the accumulation of fluid with in the abdomen.
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PARACENTESIS
• Ascites
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PARACENTESIS
• Causes of ascites
– Cancer cells
– Liver cancer
– Liver cirrhosis.
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pathophysiology
• Mechanical – obstruction of lymphatic drainage
by tumor cells
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PARACENTESIS
• Equipments
– Sterile equipments
• Antiseptic solution
• Towel with hole that is drape
• Hand towel
• Glove
• Guaze
• Swab in a galipot
• 5ml syringe
• 50ml syringe
• Specimen tube
• Drainage tubing
• Evacuated container andgetenet
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Dressing
d
set 86
PARACENTESIS
• Equipments…
– Clean equipments
• Lidocain
• Rubber sheet
• Screen
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PARACENTESIS
• Equipments
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cont……d
• Procedure
– Wash hand
– Bring equipments to the bed side
– Explain the procedure to the patient
– Screen the bed
– Position the patient
• Supine position or,
• Sitting up position or,
• High fowler’s position
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PARACENTESIS
• Supine position
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PARACENTESIS
• Sitting position
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PARACENTESIS
• High fowler’s position
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PARACENTESIS
• Procedure…
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PARACENTESIS
• Site of needle insertion
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PARACENTESIS
– For drainage
• Insert the trochar and cannula, the trochar will then removed
and the cannula will be connected with tubing for drainage
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• Procedure…
– The specimen put in the bottle and labeled then sent
to the laboratory
– At the end dress the punctured site
– Check leakage of fluid
– Remove gloves, dispose of equipment, and wash
hands.
– Help client adjust position to promotes client
comfort.
– Assess and document the time of aspiration , amount
and color of fluid removed.
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PARACENTESIS
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• After the procedure, ask the patient to lie in his bed for 4
avoid hypotension.
• Pregnancy
• Distended bowel
• Intra-abdominal adhesions.
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– Bowel may be adhered to thegetenet d
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100
• Risks of the procedure
– Post paracentesis circulatory dysfunction
– Persistent leakage of ascetic fluid
– Localized infection
• Indications
– The most common causes of pleural
effusions
• Congestive heart failure
• Cancer
• Pneumonia
• Recent surgery
– Pneumothorax
– Hemothorax
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Thoracentesis
• Complications
– Pneumothorax (3-30%),
– Hemopneumothorax,
– Hemorrhage,
– Pulmonary edema.
– infection
• Equipments
• Galipots
• Hand towel
• Gloves
• Equipments…
– Clean
• Procedure
– Explain the procedure to the patient
– Bring the equipment to the bedside and screen
– Position the patient
• Sitting up position with the arm on the
affected side above the head in order to extend
the inter costal space is preferred.
• He may also leaning forward on a pillow on
over-bed table, or can lie on unaffected side
with the arm above the head if the patient can
not sit up, in the sitting up position.
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• Procedure
– Place rubber sheet and towel behind and
expose the back of the patient
– Open the sterile set and pour the cleansing
solution into the galipot
– Scrub your hand , put glove and clean the
area
– Anesthetize the area
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Performing Thoracentesis
• Procedure…
– At the end punctured area will be
covered with a dry sterile dressing
– Record the color, amount and viscosity
of the aspirated fluid
– Leave the patient in comfortable
position
• Thoracentesis
• Monitor the client’s vital signs and respiratory status (respiratory rate
and rhythm, breath sounds, oxygenation status) hourly for the first
several hours after the thoracentesis.
• A chest tube
• Chest tube
• Indication
– Pneumothorax-accumulation of air in
the pleural space
– Pleural effusion-accumulation of fluid
in the pleural space
– Empyma-a pyogenic infection of the
pleural space
– Hemothorax- accumulation of blood
in the pleural space
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Water seal drainage system
• Chest tube
– The water seal permits drainage of air and fluid from the pleural
if07/10/2022
ordered, the addition of controlled
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Cont…..d
• Types
• Water seal
the tube.
• Complications
• Hemorrhage
• Infection
• Chest infection
• Cystic Fibrosis
• Contraindication
• Bruises on skin
• Never percuss over the kidneys, spine or female
breasts
Postural Drainage
• Positions
• Purpose
– Gastro-oesophageal reflux
– Nausea
• You have to know the part of the lung affected before performing
postural drainage.
• The patient should remain in each position 3-15 minutes sit up
and cough properly before changing to a new drainage position.
• To minimize the possibility of vomiting, drainage is best done
before meals or at least one hour after eating.
• Early in the morning and at the bed time are recommended for
postural drainage.
• Do not percuss over bare skin. Use a thin towel to cover the area
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• If you have a tracheostomy tube with a cuff, recheck it when you are
finished with PD&P. Your cuff should be:
• Laryngoscope
– Used to sweep the tongue out of the way and
align the airway so the vocal cords can be
visualized.
• Laryngoscope
– Macintosh (curved) and Miller (straight) blade
• Adult : Macintosh blade
• Small children : Miller blade
• Endotracheal tubes:-
Z-79
Disposible (Do not reuse)
Oral/ Nasal
Radiopaque marker
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Endotracheal intubation.. Equipments
• Endotracheal tubes:-
– Tube sizes.. Internal diameter (ID)
Newborn – to 4 kg - 2.5 mm (uncuffed).
1-6 months 4-6 kg – 3.5 mm (uncuffed).
7-12 months 6-9 kg – 4.0 mm (uncuffed).
1 year 9 kg – 4.5 mm (uncuffed).
2 years 11 kg – 5.0 mm (uncuffed).
3-4 years 14–16 kg - 5.5 mm (uncuffed).
5-6 years 18–21 kg – 6.0 mm (uncuffed).
7-8 years 22-27 kg – 6.5 mm ( uncuffed).
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Endotracheal intubation.. Equipments
• Endotracheal tubes:-
– Tube sizes.. Internal diameter (ID)
• 9-11 years 28-36 kg – 7.0 mm(cuffed).
• 14 to adults 46+ kg –7.0–80 mm (cuffed).
• Adult female 7.0 – 8.0mm (cuffed).
• Adult male 7.5 – 8.5 mm (cuffed).
– N.B.
• The size of the tube may also be determined by the size of the
patients little finger.
• patients below the age of 8 require uncuffed ETT due to
damage caused by the cuff in younger patients.
• Always monitor the ECG activity during intubation
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Endotracheal intubation.. Equipments
• Endotracheal tubes:-
– Depth of endotracheal tube :
– Midtrachea or below vocal cord 2cms
• Adult
Male = 23 cms
Female = 21 cms
• Children
Oral endotracheal tube = (age/2) +
12 (cm)
Nasal endotracheal tube = (Age/2) + 15 (cm)
Or 6+wt (kg)
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Endotracheal intubation.. Equipments
• Stylet
– Inserted into the ET tube to add rigidity and shape during
intubation
– Bend the tip to form a gentle curve.
– Do not insert past Murphy’s eye.
• Syringe
• Other equipment
• Suctioning
• Complications
– Trauma of the teeth, larynx and related structures.
– Hypertension and tachycardia can occur.
– Transient cardiac arrhythmias related to vagal
stimulation or sympathetic nerve traffic may
occur
– Damage to the endotracheal tube cuff, resulting in
a cuff leak and poor seal.
– Intubation of the esophagus
• Artificial airways
– Tracheoostomy tube
Nasal airways
• Tracheostomy
– Is an artificial airway consisting of a plastic
tube surgically implanted just below the
larynx into the trachea.
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• A tracheostomy
– Is a surgical procedure done to provide long-term
airway support or as an emergency procedure
when an endotracheal tube cannot be passed
successfully.
• An opening (stoma) is made in the trachea below
the larynx and the cricoids cartilage, and a semi
rigid plastic tube (tracheostomy tube) is passed
through the opening and into trachea.
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Tracheostomy
• Tracheostomy cannulae
• Indication
– Sever or recurrent upper airway
obstruction
– Patient who regularly aspirate food or
stomach content
– Patients who requires long term
mechanical ventilation
getenet d 200
• Dried secretions can completely occlude the
tube so that tracheostomy clients must be well
hydrated.
• Because it prevent the movement of air
through the vocal cords, which produce
speech, the client will not be able to talk while
these tubes are in place
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• In addition, many patients have acute and/or chronic
diseases that predispose to stagnation of
secretions. Frequent repositioning, deep breathing and
coughing, chest physiotherapy, postural drainage, oral
and parenteral hydration, and supplemental
humidification all help to thin and mobilize secretions.
• Fold the end of the tape and cut a slit then tie the
end. Trim off excess tape from the end and knot
the cut ends of the tape.
• Reconnect the patient to oxygen and reposition
for comfort.
• Discard soiled items in the appropriate
container.
• Remove and discard soiled gloves. Wash hands.
• Document the procedure, noting the
appearance of the stomal site and any exudate.
• Steam inhalation
– Is a method of introducing warm, moist
air into the lungs via the nose and throat
for therapeutic benefit.
• Respiratory benefits
– Inhaling steam is a great treatment for
respiratory complications and is
recommended for dealing with common
cold, flu, bronchitis, sinusitis, asthma, and
allergies.
– Dry air passages are moistened, and mucus
is loosened/eliminated easier by coughing or
blowing the nose.
– The moist air also alleviates difficulty
breathing, throat irritation and
inflammation.
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Steam inhalation
• Natural expectorant
– An expectorant increases the amount of
secretions, resulting in clearer secretions and as a
result, lubricates the irritated respiratory tract.
– The inhalation of steam benefits the lungs and
throat by acting like an effective natural
expectorant. This helps to relax muscles, thereby
relieving coughing.
– Inhaling steam is necessary for preventing
excessive drying of the mucous membranes.
• Improved circulation
– When the body's temperature rises, blood
vessels begin to dilate. This encourages
blood flow and overall circulation in the
body.
– The increase in circulation can provide
relief from headaches and migraines.
– The rise in temperature can also strengthen
the immune system by stimulating the
circulation of germ fighting white blood
cells.
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Steam inhalation
• Reduce Stress
– Stress causes the inner layer of the blood
vessels to constrict.
– Steam inhalation triggers the vasodilation of
blood vessels.
– When blood vessels dilate, blood flow is
increased, promoting relaxation.
– Further stress reduction can be achieved by
using specific essential oils.
• Procedure
– A common method of inhaling steam is to boil a
few cups of water and then pour the steaming
water into a large bowl.
– Next, a towel can be placed over the sufferer's
head, as he leans over the bowl of water.
– The steam will soon relieve any congestion. But
continue to inhale steam into the nasal passages
for as long as you can without discomfort.
– Inhaling steam for 10 to 15 minutes or so each
morning and evening can provide wonderful
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Steam inhalation
• Eucalypts
• Peppermint
• rosemary
• CHF patient
• Pregnancy
• Objectives
– At the end of this lesson the students will be
able to:-
• Define what lumbar puncture means
• Know the site of lumbar puncture
• List the purpose of lumbar puncture
• Discuss lumbar puncture procedure
• List and describe the contraindications and
complications of lumbar puncture
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Anatomy revision of CNS
• Classification of CNS
– Brain
• Cerebral hemispheres
• Diencephalon
–Thalamus
–Hypothalamus
• Brain stem
–Midbrain
–Pons
–Medulla
• Cerebellum
– Spinal cord
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Anatomy of HUMAN BRAIN
• Brain
Subdural space
Potential space between dura and arachnoid
mater.
Subarachnoid space
Filled with CSF
Contains the blood vessels supplying brain.
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Protection of the brain
• Procedure
– Explain the procedure to the patient
– Position the patient in the lateral
recumbent position lying on the edge of
the bed and facing away from operator.
• Objectives
– At the end of this lesson the students will
be able to:-
• Define the term bone marrow aspiration
• know the common indication for bone
marrow aspiration
• List the sites for bone marrow aspiration
• Discuss bone marrow aspiration technique
• Purpose
– For diagnosis of blood disease
especially aplastic anemia and
leukemia.
• Procedure…
– Remove the stylet and attach a 10 cc
syringe to the needle hub (patient may
experience discomfort when stylet is
removed and when suction is applied)
Objectives
– At the end of this session the students will be
able to :-
– Define the terms biopsy and liver biopsy
– List the purpose and types of liver
biopsy
– Discuss liver biopsy procedure
– Describe the contraindications and
complications of liver biopsy
• Definition
– Plugged Biopsy
• Fig. 1. Specimens of liver biopsies obtained with various sized needles and
differing techniques.
• Cylinder/stovepipe cast
– The cylinder/stovepipe cast may be used
for:
• Patellar fractures or dislocations
• Distal femoral fractures (some)
• Indications:
• Fractures
• Severe sprains
• Dislocations
• Protection of post-operative repairs
• Gradual correction of a deformity
• Contraindications:
– Open fractures
– Severe swelling
– Ulcers or draining wounds
– Compartment syndrome
– Insensate limbs
–
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• Step 4: Stockinette
• Equipments
– Cast saw
– Cast spreaders
– Bandage scissors
– Walking casts.
– Avoid dirt.
– Itching.
– Trimming.
– Knee
– Wrist
– Hip
– Elbow
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Fractures…Types…
• Definition of fixation
• Types of fixation
– Internal
– External
• Fixation materials
• Nursing intervention for patient with fixation
• Traction
– Is the application of pulling force to body to
provide reduction, alignment and rest
• Types of traction
• Skin traction
• Skeletal traction
attention .
– Pressure Ulcers
• Reference
– Redemann S, Modalities for immobilization. In
Maher A, Salmond S, Pellino T, (Ed.), Orthopaedic
Nursing, Chapter 12, 311-318, 2002. Philadelphia:
W B Saunders.
– Taylor I, Ward Manual of Traction, Chapter 2, 3, 5,
6. 1987, Churchill Livingstone.
– Traction Working Party, Traction update. Journal
of Orthopaedic Nursing, 6(4): 230-235, November
2002.
– 5National Association of Orthopaedic Nurses.
(NAON). Core Curriculum for Orthopaedic
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10. 2007. 451
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