Application of Chemical Analysis/

Analytical Chemistry in the

Cosmetics Industry
The definition of Cosmetic Product
Any substance or preparation intended to be
placed in contact with various external parts of
the human body (epidermis, hair system,
nails, lips, or with teeth and mucous
membranes of the oral cavity, with a view
exclusively or mainly to cleaning them,
perfuming them, changing their appearance
and/or correcting body odors and/or
protecting them in good condition
The Cosmetic Industry
The Cosmetic Industry
The Cosmetic Industry
The Cosmetic Industry
 Dealing with Cosmetic Regulation
 Before the cosmetic products are put in the market, the
industry has to comply with the Cosmetic Regulation by
notifying the regulatory authority.
 The notification is to ensure that their products do not cause
any damage to human health and to establish the product
information file (PIF). The PIF is a file consisting of the
qualitative and quantitative composition of the product
(name and percentage of the ingredients), raw materials
specifications, and manufacturing method as in (GMP)
Guidelines, assessment of the safety for human health of
the finished product, its ingredients, its chemical structure
and its level of exposure.
 It should also include data on the undesirable effects on
human health, supporting data for claimed benefits (efficacy
assessment), certificate of analysis to check the ingredients
and microbiological control.
Dealing with Cosmetic Regulation

In general, requirements of the

Cosmetic Regulation will cover
physical-chemical properties,
safety, microbiology and
efficacy,
 Problem Definition of Cosmetic
Products Analysis
1. Toxic Metals Contaminant Analysis such as
Lead (Pb), Arsenic (As) and Mercury (Hg)
2. Physical-Chemical Analysis such as the pH,
viscosity and color
3. Microbiological Analysis of bacteria and
other microorganisms
4. Skin Irritation Analysis
5.
 Problem Definition of Cosmetic
Products Analysis
Toxic Metals Contaminant
 Problem Definition of Cosmetic
Products Analysis
 Toxic metals can be found in moisturizer, lotion,
sun block, sunscreen, mascara, eye shadow,
face powder, lipstick, and theatrical and clown
makeup.
 Health effects may include nausea, cramps,
vomiting, skin rash, joint and bone pain, mouth
sores, cancer, stillbirths, genetic damage, immune
dysfunction, brain and learning disorders, and
impulsive and violent behavior.
 Problem Definition of Cosmetic
Products Analysis
Physical-Chemical
pH
 Skin has a different pH and to avoid and treat dry skin,
it is important to make sure that the body and skin care
products range have the proper pH balance.
 The pH of normal skin ranges from 4 to 6.5, which is
slightly acidic.
 Alkaline stronger than pH 8 is very irritating to the skin
and, unfortunately, the majority of body and skin care
products range are far too alkaline. Most skin care
soaps have a pH factor of 9 to 11.
 Problem Definition of Cosmetic
Physical-Chemical
Products Analysis
Viscosity ( in the term of Cosmetic Viscosity Science)
 Viscosity is a measure of a fluid’s
internal friction (resistance to flow)
when one layer of fluid is forced to
move in over another layer.
 Viscosity measurements are
commonly used on release
specifications for raw materials and
cosmetic formulations because these
types measurements are quick
and easy to take.
 Viscosity measurements are a key part
of creating almost any cosmetic
product.
 Problem Definition of Cosmetic
Products Analysis
Physical-Chemical
Color
The reading of color
measurement on finish
product is important to
detect the color changes
when a stability test is applied
to the product.
 Sampling Technique(s) of Cosmetic
Products
SAMPLING PROCEDURE
1. Cosmetic products shall be sampled in their original
containers and forwarded to the analytical laboratory
unopened.
2. For cosmetic products which are placed on the market in
bulk or retailed in a container different from the original
manufacturer’s pack, appropriate instructions for
sampling at the point of use or sale should be issued.
3. The number of basic samples required for the preparation
of the laboratory sample shall be determined by the
analytical method and the number of analyses to be
performed by each laboratory.
 Sampling Technique(s) of Cosmetic
Products
SAMPLE IDENTIFICATION
1. Samples shall be both sealed where taken and identified, in
accordance with the rules in force in the relevant Member
State.
2. Each basic sample taken shall be labelled with the following
information:
— name of the cosmetic product,
— date, time and place of sampling,
— name of the person responsible for taking the sample,
— name of the inspectorate.
3. A report on the sampling shall be drawn up in accordance with
the rules in force in the relevant Member State.
 Sampling Technique(s) of Cosmetic
Products
STORAGE OF SAMPLES

1. Basic samples must be stored in accordance with the

manufacturer’s instructions appearing on the label if any.
2. Unless other conditions are specified, laboratory samples
shall be stored in the dark at between 10 and 25 °C.
3. Basic samples must not be opened until the analysis is
about to begin.