STRUCTURE ANALYSIS OF GUSTDUCIN PROTEIN AND TESTING OF

PHARMACOKINETIC PROPERTIES OF COMPOUNDS

KAAVIYA JAGADHISH AND Dr. PREMNATH*
DEPARTMENT OF BIOTECHNOLOGY, KARUNYA INSTITUTE OF TECHNOLOGY AND SCIENCES
*premnath@karunya.edu, kaaviya@karunya.edu.in

ABSTRACT
The human sweet taste receptor T1R2-T1R3 (heterodimer) is responsible for sensing sweet taste and has other biological functions as well.
A protein associated with the sweet taste receptor is chosen and is structurally analyzed to know the crucial amino acids in the protein. All the
amino acids in the protein of the sweet receptor may not act as crucial amino acids. Before interaction with other molecules, the active site
molecules or binding site molecules is generally predicted. But among these active site and binding site molecules, very few amino acids act
as crucial amino acids. These amino acids may work on changing the biological functions in the human body. To identify this is we mutate
the protein and then to further prove that these are the crucial amino acids we do structural orientation analysis of the protein

OBJECTIVE
To analyze the primary, secondary and tertiary structures of the
gustducin protein and to perform anti-inflammatory and anti-
bacterial assays using an agonist and an antagonist of the human
sweet taste receptor (T1R2-T1R3 heterodimer).
PRIMARY STRUCTURE ANALYSIS
Information obtained from the protaparam database:
Number of amino aicds=354.
Molecular weight=40357.00kDa.
Theoretical PI= 5.69.
Estimated half life=30 hrs.
Formula:C1787H2813N477O548S19 TERTIARY STRUCTURE ANALYSIS
Instability index=37.55. Obtained using the SAVES tool
Aliphatic index=83.19
SECONDARY STRUCTURE ANALYSIS

Obtained using SOPMA software

ANTI-BACTERIAL ASSAY:
Anti-bacterial assay is to be conducted with the bacterial strains using
an agonist and antagonist of the human sweet taste receptor.
ANTI-INFLAMMATORY ASSAY The agonist chosen is sucrose and antagonist chosen is gymnema
This is a membrane stabilization assay in which the integrity of the sylvestre extract. After the asssay is performed we will be able to find
membrane is stabilized. This is done to prevent the release of out if the given agonist and antagonist is able to suppress the growth
enzymes during the lysis of the lysozomal membrane during of bacteria or not.
inflammation. Blood sample from humans who are unable to or
CONCLUSION
partially sense sweet taste is collected and anti-inflammatory test is
to be conducted with an agonist and antagonist of the human sweet
• Structure analysis of the gustducin protein has been completed which
taste receptor. The agonist chosen is sucrose and antagonist chosen
is to be followed by mutational analysis for identification of the
is gymnema sylvestre extract.
crucial amino acids of the proteins.
• The pharmacokinetic tests are to be performed to obtain conclusions.

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