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STEM CELL BIOLOGY 2
Guitierrez-Gonsalez et al. (2009) in their work,” Analysis of the clonal architecture of the
human small intestinal epithelium establishes a common stem cell for all lineages and reveals a
mechanism for the fixation and spread of mutations” sought to understand the nature of a
person’s stem cell architecture, Sought to understand the nature of a person’s stem cell, it
architecture, how mutation can be fixed as well as how they are spread. To achieve these
objectives, the researcher considered four main questions. First, does there exists a stem cell
from an adult that can be used in deriving all the differentiated epithelial cells? Secondly, within
a small epithelium, exactly how does a spread in mutation occur? Thirdly, how is the amount of
villi and crypts in a small bowel in a human being maintained? Lastly, as has been observed in
both the stomach and colon, does monoclonal conversion and niche succession happen in the
small intestine crypts. On the other hand, Fellous et al. (2009) in ”Locating the Stem Cell Niche
and Tracing Hepatocyte Lineage in Human Liver” sought to identify hepatocyte deficient
patches in the cytochrome c oxidase enzyme, the component of In both studies, mtDNAs were
utilized as markers for clonal expansion. However, Gutierrez-Gonsalez et al. 2009 in their study
made use of mitochondrial DNA mutation as the marker of stem cell progeny’s clonal expansion.
On the other hand, the mitochondrial DNA mutations were utilized by Fellous et al. 2009 for
After obtaining the approval to conduct their study, Guitierrez-Gonsalez et al. used 10
patients with pancreatic tumor in the study. They used normal duodenal specimens particularly
those without dysplasia or showing signs of inflammation. Similarly, Fellous et al. obtained 15
normal fresh frozen human liver blocks after ethical approval to carry out the test.
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For the study by Fellous et al. 2009, immunohistochemistry was carried out on the 4-um
Guiterrez-Gonsalez’s study was conducted on the 2 um paraffin section. They were then
allowed to dry and then dewaxed in xylene. Later they were dried by reducing the alcohol
concentrations. For both Fellous et al. 2009 and Gutierrez-Gonsalez et al.’s study, histochemistry
was done for the Cytochrome C Oxidase Succinate Dehydrogen. The analysis of mtDNA for
Guitierrez-Gonsalez et al. 2009 found that the small bowel crypts of human are clonal,
the stem cell is in a position of populating the whole stem cell zone, and that patches of the
crypts have similar mtDNA mutations. Similarly, Fellous et al. found the cytochrome c oxidase-
deficient hepatocytes patches to be clonal implying a root from the stem cell or long-lived cell.
The data from both studies are convincing about the presence of multiple or single cells
in examined region and cell’s clonal expansion in that the hypothesis for both the studies was
confirmed. Moreover, the video on Cre-lox recombination demonstrates through lineage analysis
conducting inversions, translocations, deletions and insertions in cell DNA. The Cre recombinase
is capable of splicing and recognizing specific DNA sequence referred to as the Loxp Sites. This
LoxP site comprises of thirty-four base pair and 2 bordering palindromic sequences and the eight
bp spacer region.
STEM CELL BIOLOGY 4
References
Gutierrez-Gonsalez, L., Deherogoda, M., Elia, G, Leedham, S., Shankar, A., Imber, C,
clonal architecture of the human small intestinal epithelium establishes a common stem cell for
all lineages and reveals a mechanism for the fixation and spread of mutations.” J Pathol. 217:
489–496
Fellous, T, Islam, S, Tadrous, P, Elia, G et al. (2009).Locating the Stem Cell Niche and