Usulan penelitian thesis

Muhammad Ali S M
IBD
Patofisiologi, gambaran histologis, tx/consensus (dalam animal model
apa?)
Diare
Patofisiologi, gambaran histologis, tx/consensus (dalam animal model
apa?)
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo

Male Sprague-Dawley rats (12-weeks old, 100-120 g; n=64) were

randomised into: control (KN); LPS (KL); probiotic + LPS (KL-Pr);
and sequential probiotic + LPS + probiotic (KPR-7L) groups.

Each of which was subdivided into four subgroups (n=4 rats each) that
were sacrificed on day 3, 4, 6, and 7.
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo

1. KN: sterile water

2. KL: E. coli O55:B5 LPS on day 1 + sterile water on the remaining 13
days.
3. KL-Pr: LPS on day 1 and L. plantarum IS-10506 on day 2 until the
time of sacrifice.
4. KPR-7L: L. plantarum IS-10506 for 6 days before LPS
administration, followed by the probiotic until the time of sacrifice.
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo
1 Lactobacillus plantarum IS-10506 activates intestinal stem cells in a rodent model
A.F. Athiyyah, A. Darma, R. Ranuh, W. Riawan, A. Endaryanto, F.A. Rantam, I.S. Surono and S.M.
Sudarmo

Conclusion:
Our results suggested that L. plantarum IS-10506 increased the size of
the Lgr5- and Bmi1-expressing intestinal stem cell pool and induced
the activation of these cells based on elevated ERK and β-catenin
expression to mitigate intestinal mucosal injury caused by
inflammation. These findings demonstrated that L. plantarum IS-10506
is a potentially effective therapy, especially when used prophylactically,
for the maintenance of gastrointestinal health.
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja

48 male Wistar rats (6 weeks old), divided into 4 groups, each

containing 12 rats.
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja

1. Control: normal drinking water

2. DSS: distilled drinking water containing 5% DSS + 0.5 ml 0.5%
carboxymethyl cellulose/CMC
3. DSS-G: distilled drinking water containing 5% DSS + DTCM‑G at
20 mg/kg body weight in 0.5% CMC
4. DSS-Q: distilled drinking water containing 5% DSS + DHMEQ at 15
mg/kg body weight in 0.5% CMC
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja

CgA densities
• Control: 113.0±20.4 cells/mm2 epithelium
• DSS: 319.1±32.0 cells/mm2 epithelium
• DSS‑G: 123.9±22.6 cells/mm2 epithelium
• DSS‑Q: 141.3±14.3 cells/mm2 epithelium
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja

Msi1
• Control: 4.9±0.5
• DSS: 4.8±0.5
• DSS‑G: 5.1±0.6
• DSS‑Q: 5.0±0.6 cells/crypt.
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja

Math1
• Control: 80.2±10.4
• DSS: 101.6±10.7
• DSS‑G: 99.1±8.3
• DSS‑Q: 100.1±11.3 cells/field
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja

Neurog3
• Control: 79.1±11.1
• DSS: 223.1±36.0
• DSS‑G: 103.8±12.4
• DSS‑Q: 77.3±10.9 cells/field
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja

NeuroD1
• Control: 73.3±10.7
• DSS: 217.3±24.4
• DSS‑G: 105.8±11.8
• DSS‑Q: 79.1±10.7 cells/field
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja
2 Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced
colitis
Magdy El-Salhy, Kazuo Umezawa, Jan Gunnar Hatlebakk, Odd Helge Gilja

Conclusion
The present study demonstrated that the elevated densities of
enteroendocrine cells detected in DSS‑induced colitis are probably due
to increased differentiation of early enteroendocrine progenitors
during the secretory lineage. It is likely that inflammatory processes
trigger certain signaling pathways that control differentiation of the
stem‑cell secretory lineage into mature enteroendocrine cells. In
addition, this process appears to be responsive to short‑term
anti‑inflammatory treatment. It is probable that stem cell transplantation
may be an effective treatment for patients with IBD, that have not
responded to current available treatment.
3 A probiotic complex, rosavin, zinc, and prebiotics ameliorate intestinal inflammation in an acute
colitis mouse model
Jin‑Sil Park1, JeongWon Choi, Ji Ye Kwon, Kyung ‑Ah Jung, Chul Woo Yang, Sung ‑Hwan Park and Mi ‑La Cho

Eight-week-old male C57BL/6 mice divided into three groups (5 mice

in each group)
3 A probiotic complex, rosavin, zinc, and prebiotics ameliorate intestinal inflammation in an acute
colitis mouse model
Jin‑Sil Park1, JeongWon Choi, Ji Ye Kwon, Kyung ‑Ah Jung, Chul Woo Yang, Sung ‑Hwan Park and Mi ‑La Cho

• Vehicle-treated mice were administered DSS in drinking water

• Probiotics complex-treated mice were administered the probiotics
complex (100 mg/mouse) in drinking water
• Combination drug-treated mice were administered the probiotics
complex, prebiotics, zinc, and rosavin (100 mg/mouse; probiotics
complex [1.25 mg], prebiotics [15 mg], zinc [3 mg], and rosavin [20
mg]) daily beginning 3 days after DSS administration.
3 A probiotic complex, rosavin, zinc, and prebiotics ameliorate intestinal inflammation in an acute
colitis mouse model
Jin‑Sil Park1, JeongWon Choi, Ji Ye Kwon, Kyung ‑Ah Jung, Chul Woo Yang, Sung ‑Hwan Park and Mi ‑La Cho

• Body weight
• Disease activity index (DAI) score were monitored