Name :

Dita Maryani (5213418003)

Moh. Kemal A. (5213418036)
Khusnul Aisyah Amini (5213418065)
CONTROL AND COMMAND OF BIOTECHNOLOGICAL PROCESSES
USING A FLEXIBLE APPLICATION DEVELOPED IN LABVIEW
Materials :
▸ The microorganism Haloferax mediterranei ATCC 33500
▸ The cultivation media
▸ Glucose
▸ Biomass

Some requirements were important on designing :

▸ The recipients and the pipes
▸ A command and control system for pH, temperature, and
substrate
▸ A system for monitoring oxygen and gases from bioreactor
▸ The control oxygen dissolved in the cultivation broth

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 DISCUSSON
 Control of temperature
In the first 12 hours the H. mediterranei cells are in the lag phase, they adapts to the conditions in the bioreactor;
cells don’t grow and don’t produce metabolites, the control being not representative for the bioprocess. Approximately
12 hours after the start of cultivation, the cells begin to multiply and the population develops, being in the exponential
growth phase.
 Control of pH
Practically was observed that the microorganism produces acids during normal growth and in the absence of stress
factors. “BioLab” is efficient in controlling pH during the batch phase of cultivation, the variations around the set points
being in the range ±0.02. When set point is changed, the system reacts very fast, attiring in short time the new pH value.
 Control of dissolved oxygen
The quantity of oxygen dissolved in the cultivation broth (DO2) influences strongly the cultivation of H.
mediterranei, which is aerobic. The solubility of oxygen is depending on the composition of the substrate an on the
metabolites.
 Command of feeding
The analysis of the influence of glucose concentration in the fed-batch phase on the biomass and EPS formation
allows finding the optimal fed batch cultivation conditions for EPS production.

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CONCLUSIONS
A new bioprocess for EPS production by the fed batch cultivation of the extreme
halophilic archaeon H. mediterranei is analysed. For the control and command of the fed-batch
cultivation, the parameters that influence the process are pH and temperature during the entire
cultivation and dissolved oxygen during the fed batch stage.

For the maintaining of the control parameters in certain limits during cultivation and for
the command of feeding during the fed-batch stage, the “BioLab” system was build using the
LabVIEW-software package. The results show a good control of temperature, pH and
dissolved oxygen, the application reacting fast at changes in the biosystem consisting on H.
mediterranei cells, polysaccharides and substrate rich in salts in different growth phases.

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Thanks!