Amit Raz Nayak

CERTIFICATE

This is to certify that the Mr. Amit Raz Nayak of class XII of
KENDRIYA VIDYALAYA PARADIP PORT has successfully carried out the
investigatory project entitled “To Study the digestion of starch by
salivary amylase and effect of temperature and pH on it” under the
super vision of Mr. Dharmendra Kumar Gautam for the academic
year of 2017-18. All the work related to the project is done by the
candidate himself. The approach towards the subject has been
sincere and scientific.

 Place: Paradi

Date:

External Examiner                                                           Internal Examiner

Aim:
To Study The Presence Of Oxalate Ion Content In Guava Fruit At Different
Stages Of Ripening

Theory:
Oxalate ions are extracted from the fruit by boiling pulp with dil. H2SO4.
Then oxalate ions are estimated volumetrically by the solution
with standard KMn04 solution

Requirments:
100 ml of measuring flask, pestle and mortar, beaker, titration flask,
funnel, burette, weight box, filter paper, dilute H2504, N\10
KMn04 solution and guava fruits at different stages of ripening 

Procedure:
1. Weigh 50.0 g of fresh guava and crush it to a fine pulp using
pestle mortar.
2, Transfer the crushed pulp to a beaker and add about 50 ml dil.
H2S04 to it. Boil the contents for about 10 minutes.
3. Cool and filter the contents in 100 ml measuring flask. Make
the volume up to 100ml by adding distilled water.
4. Take 10 ml of the solution from the measuring flask into a
titration flask and add 10mi of H2SO4 to it. Heat the mixture to about
60'C and titrate it against N/10 KMn04 solution taken in burette. The
end point is appearance of permanent light- pink color.
5. Repeat the above experiment with 50.0 g of 1. 2 and 3 old guava fruit
Observation:
Volume of the guava extract taken in each titration =

Weight of the guava fruit taken each time =

Normality of KMnO4 solution =

Concordant Volume:

Calculation:
For Fresh Guava

N1*V1 = N2*V2
=>N1*10 = (1/20)*X
=>Normality of oxalate,N1=X/200

1.Strength of Oxalate in Fresh Guava Extract 

 = Normality*Equivalent mass of Oxalate​
= __ g/l of the diluted extract

2.Strength of Oxalate in 1 Day Guava Extract 

= Normality*Equivalent mass of Oxalate​
= __ g/l of the diluted extract
3.Strength of Oxalate in 2 Day Guava Extract
 =  Normality*Equivalent mass of Oxalate​
= __ g/l of the diluted extract

4.Strength of Oxalate in 3  Day Guava Extract

 = Normality*Equivalent mass of Oxalate​
= __ g/l of the diluted extract

RESULT
Strength of Oxalate ion in