COMPARATIVE EVALUATION OF THE EFFECTS OF OIL PULLING, HERBAL MOUTHRINSE AND FLUORIDE MOUTHWASH ON THE CARIES ACTIVITY AND

STREPTOCOCCUS MUTANS COUNT USING ORATEST AND DENTOCULT SM STRIP MUTANS KIT

GUIDE

DR. NIKHIL SRIVASTAVA Principal & Head Department of Pedodontics and Preventive Dentistry Subharti Dental College, Meerut

CO GUIDE

DR. VIVEK RANA Reader Department of Pedodontics and Preventive Dentistry Subharti Dental College, Meerut

DR. PREETIKA CHANDNA Lecturer

Department of Pedodontics and Preventive Dentistry Subharti Dental College, Meerut

AIMS AND OBJECTIVES

Ths study is planned to with the following aims and objectives:  To evaluate the antimicrobial activity of oil pulling, herbal mouth rinses and fluoride mouthwash on the caries activity and the counts of S. mutans in the saliva of children, using Oratest and Dentocult SM kit.  To compare the antimicrobial efficacy of oil pulling, herbal mouth rinses and fluoride mouthwash on the caries activity and the counts of S. mutans in the saliva of children using the Oratest and Dentocult SM strip mutans kit.

MATERIALS AND METHODOLOGY

 The present study will be done in the department of Pedodontics and Preventive dentistry Subharti Dental College, Meerut

 Diagnostic instruments

Mouth mirror Explorer Tweezer Cotton

 For Oratest -

Sterilized milk 0.1% % aqueous solution of methylene blue Sterile beakers Screw cap test tubes 5 ml disposable syringes Sterile pipettes Test tube stand

 Sesame oil  Herbal mouthwash  Fluoride mouthwash  For streptococcus mutans - Dentocult SM

strip mutans Kit

 The study sample will be divided into three

balanced groups. Group I(study group: oil pulling), group II(study group: herbal mouth rinses) and group III(study group : fluoride mouthwash) and group IV(control group: distilled water). The children will be using the mouthrinses as per manufacturer s instruction

 For caries activity test:  The children will be asked to rinse their mouth

vigorously for 30 seconds with 10 ml of sterilized milk. The expectorate will be collected in a sterile beaker and 3 ml of expectorate is transferred with a disposable syringe to a screw cap test tube containing 0.1% methylene blue. The expectorate milk and methylene blue has to be thoroughly mixed and a mirror to be used to detect any color change(blue to white) at the bottom of the tube every 5 minutes. The time taken for the initiation of color change is recorded.

 For streptococcus mutans count:  For assessing the streptococcus mutans count in the

saliva, a paraffin tablet will be given to each subject to chew for 1 minute. Excess saliva is swallowed. The rough surface of Dentocult SM saliva strip is pressed against the saliva on the tongue and the strip has to be removed through gently closed lips. The strips are to be placed in the selective culture broth with the smooth surface clipped and attached to the cap. The vials has to be incubated in an upright position at 37 C for 48 hrs with the cap opened one quarter to allow growth of micro organisms. The growth on the strips will be assessed according to the manufacturer s chart

 Class 0 - < 10,000 CFU/ml  Class 1 - <100000 CFU/ml  Class 2 100000-1000000 CFU/ml  Class 3 - >1000000 CFU/ml.  If deemed necessary, changes will be made in

the interest of the study