“In-vitro Evaluation of the Anti-Bacterial

Property of Wound Healing Cream made

of Ipomoea batatas (Sweet Potato) Leaves
and Mangifera indica L. (Mango) Leaves
against Escherichia species”
A. H. CRUZ, M.A.R.PEREZ, & J.A. H. CASIGURAN TECHNICAL VOCATIONAL SCHOOL
FRILLES
CAPSTONE RESEARCH
GRADE 12-STEM
BACKGROUND OF THE STUDY
 Innovative wound healing product (wound
healing cream)
 Negut et al. (2018)
 Sharman et al. (2021)
 Dapar (2020)
 Chen et al. (2021)
 Parvez (2016)
STATEMENT OF THE PROBLEM
1. What will be the quality of the wound
healing cream in terms of:
a.Appearance
b.Odor
c.Consistency
STATEMENT OF THE PROBLEM

2. How effective the wound healing cream in

inhibiting bacteria?  Considering the following:
a. Ratio of aqueous extract of SPL and ML
b. The diameter of the Zone of inhibition (mm)
c. Effectivity compared to the control
 METHODOLOGY
 Observation and Agar-disk diffusion method
 On May 17, 2022, the finished product was
evaluated its antibacterial property at the Regional
Animal Disease Diagnostic Laboratory located in
Cabañgan, Camalig, Albay.
 METHODOLOGY
Procedures:
Collection of raw materials

Washing and drying the leaves

 METHODOLOGY
Sterilizing and disinfecting

Extraction
 Grinding
 Decoction
 METHODOLOGY
Procedures:
Making wound healing cream
 Measuring
 Melting
 Blending
 Storing
Formulation of the Wound Healing Cream

 Ingredients  Quantity  Percentage by

mass
Olive oil  80 ml/ g  31.86%
Plant-based extract  40 ml/ g 26.1%
Beeswax 15g 6.64%
 Vegetable Glycerin 3 tbsp./ g 13.27%
RESULTS
Evaluation
Parameter
Observation

WHC-1 WHC-2 WHC-3

Color Dark goldenrod Coffee Sepla

Odor Myrrh Oil odor Myrrh Oil odor Myrrh Oil odor

Consistency Smooth Smooth Smooth

 Yellow Canary Gold Sunglow Jonquil Mikado Mustard Harvest Gold Goldenrod Dark Russet Sepla Coffee
Yellow (metallic) goldenrod
gold

R
E
 RESULTS
ITEM SAMPL FIELD SAMPLE ID TEST AST (ANTI-
MICROBIAL
S NO. E NO. Sample Description ORGANISM SENSITIVITY TEST)
Zone of Inhibition
(mm)
Trial 1 Trial Trial 3
2
1 05538 WHC-1:Wound Healing Cream Escherichia 0 mm 0 mm 0m
w/ Mango Leaves sp.
2 05539 WHC-2:Wound Healing Cream w/ Escherichia 0 mm 0 mm 0 mm
Sweet Potato Leaves sp.

3 05540 WHC-3:Wound Healing Cream Escherichia 0 mm 0 mm 0 mm

w/ Combination sp.
4 05541 C. Trimycin Escherichia 9 mm 13 11
sp. mm mm
 CONCLUSION

1.What will be the quality of the wound healing cream in

terms of:
a.Appearance
b. Odor
c. Consistency
 CONCLUSION
Based on the results, the creams made of Ipomoea batatas leaves
and Mangifera indica L. leaves namely, WHC-1, WHC-2 and WHC-3
have good quality considering its color, odor, and consistency. The
color of the creams was affected by the extract used and its
consistency were affected by the amount of the beeswax added.
 CONCLUSION
2. How effective the wound healing cream in inhibiting bacteria?
Considering the following:

a. Ratio of aqueous extract of SPL and ML

b. The diameter of the Zone of inhibition (mm)

c. . Effectivity compared to the control

 CONCLUSION
The result of the laboratory test regarding the
antibacterial property of the three creams against Escherichia
species was inactive with 0 mm zone of inhibition.
Therefore, the researchers concluded that the cream is not
effective in inhibiting the tested bacteria.