ADVANCEMENT STATE

Microbial reduction on E.coli

 Extracts preparation
• 3 extracts were prepared in three separate ways :
first extract containing lyophilized OP using distilled water as a solvent 1:4(w/v)
second extract containing a ratio of 1:5 (w/v)
Third extract using a 60% ethanol solution
• The extracts were placed in a water bath at 45°c for 3h
• The they were centrifuged at 7000g for 15min
• The extracted liquid was filtered using a vacuum filtration system
Microbial reduction results