pH Measurement

Presentation made by:

Dane Jeciel F. Gomez
Pearl Diane E. Mercado
Myeisha Gabrielle L. Seda
 What is pH?
PH MEASUREMENT

The concentration of
hydrogen ions is determined by
pH. It is a way to gauge how
basic (alkali) or acidic aqueous
solutions or other liquids are. It
is determined through the use
of a pH meter.

2
PH MEASUREMENT
What is pH
meter?
A pH meter measures the activity of hydrogen ions in solutions,
or the acidity or alkalinity of a solution. pH level is then used to
describe the concentration of hydrogen ions, typically ranging from 1-
14. The ratio of the positive hydrogen ion and hydroxyl ion (or the
negative hydroxide irons) concentration ([H+] and [OH-],
respectively) is directly related to this pH measurement. For example,
the pH of pure water is neutral since the ratio of positive hydrogen
ions to negative hydroxide ions are equal. A neutral solution has a pH
3

of 7.
How does a pH meter work?
Three different components make up a pH meter: an internal
electrode, a reference electrode, and a high input impedance meter. The
internal electrode and reference electrode are frequently present in glass
probes. The reference electrode is frequently constructed of the same
materials as the internal electrode, which is a silver wire covered with
silver chloride (Ag/AgCl wire). A buffer solution with a pH of 7 is
located inside the probe. The [H+] difference between the reference
buffer inside the probe and the sample solution is the measured pH.
PH MEASUREMENT How can you determine if the
solution is acidic or alkali?
As stated, pure water has a pH level of 7, which we consider as neutral.
Acidic solutions have a pH lower than 7 and contain more hydrogen ions (H+)
than pure water. Meanwhile, a solution is basic (alkaline) and has a pH higher
than 7 if it contains more hydroxide ions (OH-) than water. It is much
easier to determine through
the use of pH scale.

5
DEMONSTRATION
 MATERIALS
PH MEASUREMENT

7. Bleach
1. pH meter
8. Coke
2. Pure water
9. Orange juice
3. Sea water
10. Beakers
4. Rain water
11. Buffers (pH 4.0 , 7.0 and 10.0)
5. Milk
12. Distilled water
6. Soapy water
13. Vinegar

7
PH MEASUREMENT PROCEDURE
1. Turn on your pH meter and give it enough time to warm up before you can start
calibrating and using it. This procedure should typically take about 30 minutes. For
specific times, consult the user's guide for your pH meter.

2. Clean the electrodes using distilled water and under an empty waste beaker. Make
sure to rinse the electrode in a discarded beaker rather than the one that will be used for
calibration. Avoid rubbing the electrode as it has a sensitive membrane that surrounds
it. Instead, pat it dry using tissue paper.

8
PH MEASUREMENT PROCEDURE
3. Prepare the buffers for calibration. The chosen buffers are 4.0, 7.0 and 10.0 pH. The
buffer that has 7.0 pH would be the neutral buffer. The second should be near the
expected sample. The buffer that has 10.0 is best for measuring bases while the one
with 4.0 is best for measuring acidic samples. Discard them after use and do not return
them to their original container.

4. Place the electrode in the buffer with a pH of 7, then begin reading. Press the
"measure" or "calibrate" button after inserting the electrode into the buffer to begin
reading the pH. Allow the pH reading to stabilize before letting it sit for approximately
9
1-2 minutes.
PH MEASUREMENT

5. Once a stabilized reading is observed, press the measure button a second time to set
the pH meter to the buffer's pH value. More precise and accurate readings can be
obtained by setting the pH meter once the reading has stabilized.

6. After this process, rinse and clean the electrode just like in step 2.

7. Repeat steps 4 and 5 but this time with the second and appropriate buffer that has a
pH close to the sample.

10 8. Rinse and clean again your electrode.

PH MEASUREMENT

9. Using the sample, repeat steps 4 and 5 again. After pressing the
measure button again, it will now display the pH level of the sample.

10. Do not forget to clean the electrodes after use.

11. Do these procedures for each sample (pure water, vinegar, seawater,
rainwater, milk, soapy water, bleach, coke, and orange juice).
11
 How important is measuring pH
levels?
PH MEASUREMENT

A solution's pH is a significant indicator of its

chemical composition. Nutrient availability, biological
function, bacterial behavior, and chemical behavior
can all be impacted by pH. It is also important for
keeping the health of the consumers and the food
12 industry. It is also such a big help in the medicinal field. 
THANK YOU
 Sources
PH MEASUREMENT

Electrical4U. (2022, October 26). pH measurement. 

https://www.electrical4u.com/ph-measurement/?fbclid=IwAR27b-hEAz5P_rt7VewJENRse_Lu7K23scOcd
pFxw-_DmP3cOG-BKQq1Ygk

Iowa State University. (2019, April 4). pH meter instrument.

https://group.chem.iastate.edu/Holme/augmented-reality-in-educational-laboratories/ph-meter/
Iowa State University. (2019, April 5). pH meter safety.
https://group.chem.iastate.edu/Holme/augmented-reality-in-educational-laboratories/ph-meter/safety.php
?fbclid=IwAR0thKpfNouCDU7HxcjXG-Q9gX2WKD5qc7e3wCac-lUAz3JWVTeRBL-pzqI
 

Hach. pH. https://www.hach.com/parameters/ph

Britannica, T. Editors of Encyclopaedia. (2022, November 9). pH. Encyclopedia Britannica. https://
www.britannica.com/science/pH
14
 Sources
PH MEASUREMENT

WikiHow. (2021, September 12). How to calibrate and use a pH meter.

https://www.wikihow.com/Calibrate-and-Use-a-pH-Meter#:~:text=Place%20your%20electrode%20in%2
0the%20appropriate%20buffer%20for%20your%20sample,is%20placed%20in%20the%20buffer.&text
=Set%20the%20pH%20a%20 second,by%20pressing%20the%20measure%20button
.

Apera Instruments. (2017, November 15). Why pH is important.

https://aperainst.com/blog/why-ph-is-important/#:~:text=pH%20is%20an%20important%20quantity,and
%20the%20behavior%20of%20chemicals
.

In K. A., Ranson, K. W., Leish, R. B., Roth & E. F., Sparn (eds.). (1998.). Grolier International
Encyclopedia Deluxe Home Edition. (vol. 15).(p. 233). Philip Friedman.

15