Directly and indirectly determinable

rate constants in Michaelian

enzymecatalyzed reaction
sura
introduction

• Enzymes are essential biological catalysts that play a crucial role in many biochemical
processes. Understanding the kinetics of enzyme-catalyzed reactions is important for
gaining insights into the mechanisms of these processes and for developing new drugs and
therapies. the determinable rate constants involved in Michaelian enzyme-catalyzed
reactions, shedding light on the intricacies of these reactions and their potential
applications.
The problem
• the determination of directly and indirectly determinable rate
constants in Michaelian enzyme-catalyzed reactions. Enzyme
kinetics is a complex field that involves understanding the rates of
enzyme-catalyzed reactions, which can be influenced by various
factors such as substrate concentration, pH, temperature, and
enzyme concentration.
• The Michaelis-Menten equation is commonly used to describe the
kinetics of enzyme-catalyzed reactions, but it only provides
information on the initial reaction rates. To fully understand the
kinetics of these reactions, it is necessary to determine both
directly and indirectly determinable rate constants.
The goal of the

• 1) To derive equations for the first order rate constants,

k+2 and k-2, for the
activation of ES and its deactivation, respectively;
• 2) to quantify by calculation the first order rate constant
for product release;
• 3) ultimately quantify the rate constants, k-2 and k+2;
• 4) to advise the reactor, process, chemical engineers,
etc. in different industrial concerns on the usefulness of
k-2 and k+2
•Chemicals
•The enzyme which was assayed is
Aspergillus oryzae alpha-amylase

•insoluble potato starch, was the

substrate;
conclusion

• proposes a new kinetic scheme that is more similar to

the kind of scheme to be explored in this research and
uses mathematical models to derive equations for the
rate constants involved in this scheme.
• The results of this research can have implications for
various fields such as biochemistry, pharmacology, and
biotechnology.
• it provides a comprehensive understanding of the
kinetics of enzyme-catalyzed reactions and can
contribute to the development of new drugs and
therapies that can target specific enzymes and
biochemical processes.
thanks