PRAYER

Dear Lord and Father of all, Thank you for

today. Thank you for ways in which you
provide for us all. For Your protection and
love we thank you. Help us to focus our
hearts and minds now on what we are
about to learn. Inspire us by Your Holy
Spirit as we listen and write. Guide us by
your eternal light as we discover more
about the world around us. We ask all this
in the name of Jesus. Amen.
B I O LO G I C A L T E C H N I Q U E S

ANTIMICROBIAL
ASSAY TEST
Antimicrobial
assay test
Laboratory procedure performed
by medical technologists to
identify which antimicrobial
regimen is specifically effective
for individual patients.
 Antimicrobial testing
method
Disc diffusion
method identify resistant bacteria by creating a
gradient of concentration around a disk
impregnated with antibiotics. The
gradient of antibiotics prevents the
growth of bacteria creating an
“inhibition zone” around the disk.

Minimum Inhibitory Concentration

MIC test that determines the lowest
concentration of an antimicrobial agent
needed to inhibit the visible in-vitro
growth of a challenge microorganism.
Phytochemical
Phytochemical
Phyto
Greek word for plants

Phytochemicals or Phytonutriens
biologically active, naturally occurring
substances in plants that have protective or
disease preventive properties.
provide pigment to the plants.
nonessential nutrients
Phytochemicals are defined as bioactive nutrient
plant chemicals in fruits, vegetables, grains,
and other plant foods that may provide
desirable health benefits beyond basic nutrition
to reduce the risk of major chronic diseases
(Liu, 2004).
Phytochemical analysis
detection of the bioactive principles
present in medicinal plants and
subsequently may lead to drug discovery
and development
 Phytoconstituents
non nutrients active plant chemical
compounds or bioactive
compounds
protect plants from harmful
agents such as insects and
microbes as well as stressful
events such as ultraviolet (UV)
radiation and extreme
temperatures.
 TYPES OF PHYTOCONSTITUENTS

PHENOLICS

Legumes Tea Broccoli

 TYPES OF PHYTOCONSTITUENTS

ALKALOIDS

Caffeine Tea Cacao

 TYPES OF PHYTOCONSTITUENTS

SAPONINS

Pea Soybean Soapwort

 TYPES OF PHYTOCONSTITUENTS

GLYCOSIDES

Anthocyanin Oleander Dogbane

 TYPES OF PHYTOCONSTITUENTS

TERPENES

Cannabis Spanish Sage Citrus

 TYPES OF PHYTOCONSTITUENTS

TANNINS

Maple Eucalyptus Willow

 TYPES OF PHYTOCONSTITUENTS

ANTHRAQUINONE
S

Senna Yellow Dock Buckthorn

 TYPES OF PHYTOCONSTITUENTS

STEROIDS

Trigonella Withania Smilax

 TYPES OF PHYTOCONSTITUENTS

ESSENTIAL OIL

Patchouli Lavender Peppermint

A healthy outside starts
from the inside.

-Robert Urich
THANK
YOU!
References:

https://www.ncbi.nlm.nih.gov/books/NBK539714/

https://www.slideshare.net/enigmisha/phytochemicals-52245189

http://www.aelsindia.com/rjcesapril2016/2.pdf