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  • 32 Jaundice, VanD RXN and Bilirubin Metabolism

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    Sanchita
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    Bilirubin is produced from the breakdown of heme in red blood cells. The liver conjugates bilirubin so it can be excreted in bile and urine. The Van den Bergh reaction uses a mixture of sulfanilic acid and sodium nitrite to measure bilirubin levels in the blood and determine if jaundice is caused by elevated conjugated or unconjugated bilirubin. A direct positive reaction indicates conjugated bilirubin is elevated, while an indirect positive reaction requires methanol and shows unconjugated bilirubin is elevated.

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    32 Jaundice, VanD rxn and bilirubin metabolism

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    Bilirubin is produced from the breakdown of heme in red blood cells. The liver conjugates bilirubin so it can be excreted in bile and urine. The Van den Bergh reaction uses a mixture of sulfanilic acid and sodium nitrite to measure bilirubin levels in the blood and determine if jaundice is caused by elevated conjugated or unconjugated bilirubin. A direct positive reaction indicates conjugated bilirubin is elevated, while an indirect positive reaction requires methanol and shows unconjugated bilirubin is elevated.

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    31 views14 pages

    32 Jaundice, VanD RXN and Bilirubin Metabolism

    Uploaded by

    Sanchita
    Bilirubin is produced from the breakdown of heme in red blood cells. The liver conjugates bilirubin so it can be excreted in bile and urine. The Van den Bergh reaction uses a mixture of sulfanilic acid and sodium nitrite to measure bilirubin levels in the blood and determine if jaundice is caused by elevated conjugated or unconjugated bilirubin. A direct positive reaction indicates conjugated bilirubin is elevated, while an indirect positive reaction requires methanol and shows unconjugated bilirubin is elevated.

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    Bilirubin Metabolism & Jaundice

    BILIRUBIN METABOLISM
    Jaundice - Definition
    UGT: Uridine diphosphate-glucuronyltransferase
    Van den Bergh reaction
     Van den Bergh reaction is a chemical reaction used to measure bilirubin levels
    in blood

     It determines the amount of conjugated bilirubin in the blood and is highly


    useful in understanding the nature of jaundice.

     Van den bergh reagent is mixture of equal volumes of sulfanilic acid (in dilute
    HCL) and sodium nitrite.

     Priniciple: Diazotised sulfanilic acid reacts with bilirubin to form purple


    colored azobilirubin
     Direct Positive: Conjugated bilirubin produces color as it is soluble in reagent.

     Indirect Positive: Unconjugated bilirubin do not produce color directly as it is


    insoluble but it gives color when mixed with methanol.

     If both conjugated and unconjugated bilirubin are present the reaction is termed a
    biphasic reaction.

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