Beckman Coulter’s VCS

Technology

Prepared by
M.A.Ghori
 COULTER® VCS
TECHNOLOGY
VCS Technology is the most powerful
tool available for blood cell analysis.
An acronym for Volume,
Conductivity and Scatter, this
proprietary technology offers the
greatest sensitivity, specificity and
efficiency of any cell analysis system
available today.
 VOLUME MEASUREMENT

VCS utilises the

Coulter Principle of
counting and sizing
to measure the
volume of the cell by
using Direct
Current (DC) across
the two electrode in
a flow cell.
 CONDUCTIVITY
MEASUREMENT

When a cell is
exposed to high
frequency current
(RF) , it no longer
remains insulator,
the RF energy
penetrate into cell
and reveal
information about its
size and internal
structure.
 SCATTER MEASUREMENT

As cells are passing

through the flow cell in
single stream they are
exposed to a laser
beam, when laser strike
them they scatter light.
The light scatter at
angles between 10 and
70 deg is used by VCS
instruments.
The scattered light gives information about cell
surface and granularity
 SIMULTANEOUS
MEASUREMENTS

VCS is the only single

channel analysis that
uses 3 independent
energy sources to probe
approximately 8,192
cells in their near native
state.Each providing 256
channels of resolution
over 16,700,000
channels in all.
VCS sample flow
 NE
EO
MO

LY

DF2

DF1 SCREEN
 MO
NE
EO
BA

LY

DF1

DF2 SCREEN
 MO

BA

LY

DF1

DF3 SCREEN
VOLUME COMPENSATION

BEFORE VOLUME AFTER VOLUME

COMPENSATION COMPENSATION
 WBC DIFF SCATTERPLOT

MONOCYTE EOSINOPHILS
11 OR MORE CELLS
5 TO 10 CELLS

2 TO 4 CELLS
1 CELL
NEUTROPHIL

BASOPHIL
behind lymphocyte

LYMPHOCYTE
 Ne

Mo

Eo
Ly

DF1 SCREEN AS SEEN NORMALLY WITH THE RESULT

 Ne
Mo
Eo
Ba
Ly

DF2 SCREEN CAN BE ACCESSED BY PRESSING Alt+F2 KEYS

 Mo

Ly
Ba

DF3 SCREEN CAN BE ACCESSED BY PRESSING Alt+F3 KEYS