ASSESSMENT OF ANTIOXIDANT POTENTIAL OF SELECTED

PLANT STEMS TRADITIONALLY USED FOR ORAL CARE

Introduction
Results

Oral care is crucial for overall health, but oxidative stress caused by reactive
Total Phenolic Content TPC
oxygen species (ROS) and free radicals poses challenges. Antioxidants have
gained attention for their ability to neutralize ROS, reducing oxidative stress 250 800
and preventing damage. Natural sources of antioxidants, like traditional plant 700
200
extracts, are being explored. These plants contain bioactive compounds,

TPC mg GAE/ 100mg FM

TPC mg GAE/100 mg FM
600
including polymers, with promising antioxidant properties. Assessing the 150 500
antioxidant activity of these polymers provides insights into their potential as 400
100
preventive and therapeutic agents in oral health. This research aims to 300
identify effective and sustainable alternatives to conventional oral care 50 200
products by incorporating natural compounds. Evaluating the antioxidant 100
properties of polymers from traditional plants can lead to the development of 0
Zaitoon Sukh Sheesham Neem Kikar Safeda Amaltas 0
Chain Zaitoon Sukh Sheesham Neem Kikar Safeda Amaltas
novel antioxidant-based strategies for oral care, promoting oral health and Chain
Stem extracts from shredded samples
well-being. The findings can contribute to the use of traditional plant extracts Stem Extracts from powdered samples
as a source of natural antioxidants in oral care. Miswak, a teeth-cleaning twig
made from the Salvadora persica tree, has been traditionally used for oral
care. Studies indicate that Miswak extract exhibits lower antimicrobial Total Flavonoid Content TFC
activity compared to conventional oral disinfectants. This article highlights
60 120
that besides Salvadora persica, other plants like Sheesham, Neem, Zaitoon,
50 100
Kikar, Safeda, Sukh Chain, and Amaltas are also used as Miswak in Pakistan.

TFC mg QE/ 100mg FM

TFC mg QE/ 100mg FM

40 80

30 60

20 40

10 20

0 0

Objective
Zaitoon Sukh Sheesham Neem Kikar Safeda Amaltas Zaitoon Sukh Sheesham Neem Kikar Safeda Amaltas
Chain Chain

Stem Extracts from shredded samples Stem Extracts From powered samples

The aim of this study is to Screen the antioxidant potential of polymers

extracted from various extracts of traditional plant stems and identify  DPPH Radical Scavenging Activity
potential sources of novel antioxidants for oral care
100
100
90
DPPH Percentage scavenging

90

DPPH Percentage scavenging

80 80
70 70

Methodology 60 60
50
percent

50

percent
40
40 30
30 20
Plant List 20 10
10 0
ar s
 Zaitoon on ha
in
ha
m
ee
m
ik ed
a
al
ta
0 ito C s N K Sa
f
 Sukh chain in r a s Za ee m
on ha am eem ika ed al
ta kh Sh
A
to sh f
Su
Za
i C ee N K Sa m
 Neem k h
Sh A
Su Stem Extracts from powdered samples
 Sheesham Stem Extracts from shredded samples

 Kikar  ABTS Radical Cation Scavenging Activity

 Safeda
 Amaltas 80
Kikar Vachellia 90
ABTS radical cationscavenging

70 80
ABTS radical cation scavenging percent

70
Antioxidant 60 60
50
. Polymers 50
percent

40
40 30
 Lignin 20
30
 Tannins 10
0
 Flavonoids 20
in ar a s
on ha ha
m
ee
m
ik ed al
ta
 to f
Flavanones 10
Za
i C ee
s N K Sa m
kh Sh
A
 Anthocyanins 0 Su
 Proanthocyanidins Stem Extracts from powdered samples

 Cellulose Stem Extracts from shredded samples

This methodology aimed to obtain purified polymers from the

selected plant materials for further analysis of their antioxidant
activity. Conclusion
Results of the current study exhibited that powder samples show high antioxidant
potential as compared to shredded samples. Among the tested samples highest phenolic
Antioxidant Assessment
content was recorded for C. fistula (Amaltas) 700 mg GAE/ 100 g and the Lowest in V.
 The antioxidant activity of the extracted polymers was assessed nilotica (Kikar) 30 mg GAE/ 100g. For flavonoid contents, M. pinnata (Sukh chain)
through the determination of Total Phenolic Content (TPC) using the
Folin-Ciocalteu assay and Total Flavonoid Content (TFC) using came out with the highest value 114 mg QE/100g while Azadi rachtaindica (Neem) with
colorimetric assays.
 Various techniques including DPPH radical scavenging assay, ABTS lowest flavonoid content 26mg QE/100g. The highest radical scavenging potential was
assay, and FRAP assay were employed to evaluate the antioxidant
activity of the polymers.
observed in C. fistula (Amaltas) and Eucalyptus (Safeda) and Lowers in Azadi
 Statistical analysis was performed on the obtained results to identify
rachtaindica (Neem). Therefore, on the basis of the current study on antioxidant
plant sources with potent antioxidant activity and assess their potential
application in oral care. potential, we can recommend the stem of C. fistula (Amaltas), and Eucalyptus (Safeda)
for oral care.

Author: Presenter:
1.Nisba Rani, Department of Chemistry, The University of Lahore, Lahore, Pakistan Nisba Rani, Department of Chemistry, The University of Lahore, Lahore, Pakistan
2.Sumera Saleem Department of Chemistry, University of Central Punjab, Lahore, Pakistan Corresponding Author: nisbarani1998@gmail.com
Corresponding Author: nisbarani1998@gmail.com