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INTRODUCTION
Biosurfactants are very important because they can replace chemically synthesized surfactants in many industrial applications 1. These biosurfactants can be
obtained from renewable substrates, that are readily available and inexpensive 2. Surfactants based on carbohydrates are biocompatible, completely biodegradable, non-
toxic, without odour and taste and non-irritant for skin. For this reason they are used in pharmaceutical, medicine, bioremediation and food areas 3.
The purpose of this paper is to develop an efficient enzymatic method for the synthesis of carbohydrate esters of fatty acids. Development of synthetic methods
involve improving the outcome of the reaction rate, conversion, regioselectivity, productivity, safety and catalytic stability. The main objective is the development of
enzymatic methods for obtaining glucose esters with fatty acids, using solvents as adjuvants in the esterification process. The experimental program followed the
influence of various parameters on the conversion of fatty acids (temperature reaction; enzyme concentration; type of fatty acid; solvent type).
70 70 70
60 60 60
50 50 50
40 40 40
30 30 30
20 20 20
10 10 10
0 0 0
0 2 4 6 8 10 0 2 4 6 8 10 0 2 4 6 8 10
Time, h Time, h Time, h
The influence of different solvents on fatty acid The influence of the amount of enzyme in the Influence of reaction temperature on
conversion on the enzymatic esterification of glucose conversion of fatty acid conversion of fatty acid
with myristic acid
CONCLUSIONS
•The advantages of enzymatic synthesis are higher yields and milder reaction conditions, resulting in high quality products with low energy consumption.
•The results obtained in the experiments show the possibility of obtaining of glucose fatty acid esters by enzymatic esterification.
• Increasing the temperature leads to the increasing of acid conversion, but temperatures higher than 60 ° C denaturation of the enzyme can occur.
• Increasing the concentration of the enzyme leads to an increase in fatty acid conversion. A significant increase occurs up to 12% enzyme concetration
and then is just a small increase and does not justify the use of large amounts of enzyme.
•Glucose esters of fatty acids are used as emulsifiers in foods, cosmetics and pharmaceuticals.
REFERENCES
1. Kosaric N., Pure & Appl. Chem., 64 (11), pp. 1731-1737,1992.
2. Baccile, N.; Nassif, N.; Malfatti, L.; Van Bogaert, I. N. A.; Soetaert, W.; Pehau-Arnaudet, G.; Babonneau, F. Green Chem., 12, 1564–1567, 2010.
3. Chang, S. W., Shaw, J. F., New Biotechnology, 26 (3-4), pp. 109-116, 2009.
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