SETTING UP OF IUI LAB

Dr. Suryakant Hayatnagarkar

Cryo Cell India Pvt. Ltd.

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 Rationale for doing I.U.I
 Optimization of gamete availability at the
site of fertilization
 Reduction in Distance traveled by Sperm
 Tackling of occult Seminal problems
 Optimization of ovulation & Endometrium

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 Ideal Goals of Sperm
Processing
 Select highest number of motile sperms
 Remove Seminal Plasma & Prostaglandins
 Remove dead , damaged & abnormal sperms
 Remove white cells & round cells
 Induce Capacitation & Hyperactivation
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 IUI is an effective,non invasive,
relatively simple & inexpensive
method of treatment. It can be
provided easily in simple setups.
Careful selection & counseling of
couples is essential.
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 SETTING UP IUI LAB
Essential requisites of setting up
ART services
Good lab design
Good service delivery

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 SETTING UP IUI LAB
Place
Equipment
Personnel
Facilities

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 PLACE
 One room
preferably 100
sq feet
 Air conditioned
 Away from
busy areas, dust
 Preferably in
basement
 Near OT or
insemination
room
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SECTIONS IN IUI LABORATORY
Semen analysis and processing
laboratory
Semen sample collection cabin
(Masturbatory)
IUI procedures room
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 DESIGNING OF LAB

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 OTHER ANCILLARY AREAS
Reception and waiting area
Subfertility clinic
Male clinic/ Andrology clinic
Doctor’s cabin
Transvaginal Ultrasonography room
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 TESTING EQUIPMENT
Binocular Microscope
OR
Trinocular microscope
Microscope should have camera
port and 20x objective
Phase contrast accessory would
be ideal for semen analysis
laboratory
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 TESTING EQUIPMENT
SPERM COUNTING CHAMBER
Neubaur’s Chamber
Cryocell sperm counting chamber
Makler chamber

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 PROCESSING EQUIPMENT
CENTRIFUGE
 Swing out rotor
 RPM meter
 Timer
 Cooling/ auto breaks not
essential

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 PROCESSING EQUIPMENT
INCUBATOR
TEST TUBE WARMER
 Digital indicator and PLC
controller desirable
 CO2 incubation not essential
for IUI work.

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 ANCILLARY EQUIPMENT
Laminar
Flow
Bench
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 ANCILLARY EQUIPMENT
Refrigerator
For storage of
media and kits
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 ACCESSORIES FOR
PROCESSING

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 Media Used In Sperm
Processing
 Use media manufactured by reputed manufacturer, do
not use from cottage manufacturer or fly-by-night
operators
 Do not use media in open package, or bulk pckings as
it is likely to be contaminated before finishing.
 Insist on quality certification / check for manufacturers
instructions before using.

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 Media Used In Sperm
Processing
 HAM F-10
 EBSS
 HUMAN TUBAL FLUID MEDIUM
Additives
Pentoxyphyline
Albumin

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Advantages of Density Gradient
Centrifugation
1. Can be used for both normal and oligospermic samples
2. Concentrates highly motile sperm in the pellet.
3. Effectively removes seminal plasma, round cells and
dead sperm
4. Reduces oxidative damage to sperm.
5. Viscous semen samples can be processed.
6. Enhances percentage of spermatozoa with normal
morphology.
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 PERSONNEL
 Trained Gynecologist
 Trained Technician
 Other support services
– Assistants
– Sonologist

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WHAT CRYOCELL CAN DO FOR
YOU
Supply Quality reagents and
Kits
Help in designing IUI lab and
selecting equipments for the lab
Help in procuring the equipment

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WHAT CRYOCELL CAN DO FOR
YOU
Train Gynecologists in procedures
Train Technicians in processing
techniques
Supply tested Quarantined Frozen
Donor Semen samples
Troubleshooting
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