Professional Documents
Culture Documents
To provide and ensure the efficient processing of diagnostic tests and techniques
that is accurate, reliable, reproductive and clinical relevance.
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5.0 CALIBRATION
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8.0 CALCULATIONS
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11.0 REFERENCE
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12.0 DISTRIBUTION
Sterile containers, sterile aerobic swab with transport media, sterile urine and
stool containers and any sterile tubes. No fixative is added to microbiology
samples.
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7.1Blood specimen
Blood must be withdrawned aseptically.Change the needle after
drawing the blood and putting it to the blood culture bottle.Skin
must be clean with proper disinfection to avoid specimen
contamination with skin flora.Disinfect the rubber diaphragm of the
culture bottle with the alcohol.Do not use iodine.
7.2Abscess, Aspirated Fluid
Syringe aspiration is preferable.
7.3Skin Lesions and Wounds
Swabs with transport media is used.
7.4Specimen from Throat, Nose, and Ear samples
These are taken with swabs in a transport media.
7.5Specimen from High Vaginal,Cervical,Vaginal:
These are done in the clinic and is collected thru swab with transport
media.
8.0 CALCULATIONS
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11.0 REFERENCE
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12.0 DISTRIBUTION
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7.1 The lab staff will ensure that all tests are performed and reported within the
prescribed
turn around time.
8.0 CALCULATIONS
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11.0 REFERENCE
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12.0 DISTRIBUTION
Approved by:
All samples taken from the clinic with transport media such as vaginal
swabs,cervical swabs,high vaginal swabs,throat swabs,eye swabs,nasal
swabs,pus. Urine,prostatic fluid and other discharge too.
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7.0 PROCEDURE
7.1 MICROSCOPY
Identification of bacteria is primarily knowing if it is in the group of bacilli
or cocci by doing gram staining.
7.1.1 COCCI
Cocci has two categories. The staphylococci and Streptococci. For
identifying this type of organism, we will do catalase test using 20
vol.Hydrogen Peroxide. The formation of bubbles is a positive for
catalase which is known to be Staphylococci. Coagulase negative
organisms are the Streptococci.
7.1.2 BACILLI
These are the rod-shaped bacteria that could be distinguished by the gram
stain reaction.
7.1.2.3 COCCO-BACILLI
Some bacteria are pleomorphic cocci-bacilli represented by
Haemophilus specie.
7.1.4 SENSITIVITY
Sensitivity of identified pathogenic organisms done. Each sample specimen
has its own specific antiobiotic dics, incubated and read according to the
zone of inhibition as to the sensitivity.
8.0 CALCULATIONS
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11.0 REFERENCE
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12.0 DISTRIBUTION
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7.1 Stain heat fixed smears with reagent I (Crystal Violet) for 1 minute.
7.2 Wash off with tap water and flood slide with reagent 2 (Iodine) for 2
minutes.
7.3 Wash off with tap water and decolorize. Smear with reagent 3 (Decolorizer)
for a few seconds only.
7.4 Wash well in water and counter stain with reagent 4 (Safranine) for 2
minutes.
7.5 Wash with tap water and allow to dry.
7.6 Examine under microscope using oil immersion objective.
8.0 CALCULATIONS
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10.0 INTERPRETATION OF RESULTS
11.0 REFERENCE
6.0 DISTRIBUTION
Approved by:
Dr. Mustafa Hameem
4.2 TB Decolorizer
Ethanol 97 %
HCC 3%
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7.1 Prepare a thin smear and heat-fix by gently heating in the flame.
7.2 Flood fixed smear with Carbol Funchin and let it stand for 10 minutes.
Do not heat.
7.3 After this period, add some of the decolorizer washing immediately
afterwards with water. Repeat this operation until smear becomes pale
pink.
7.4 Finally cover the slide with methylene blue for 5 minutes.
7.5 Rinse with water and air dry.
7.6 Examine under oil immersion objective
8.0 CALCULATIONS
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Acid alcohol resistant mycobacteria are seen as red on pale blue background.
11.0 REFERENCE
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12.0 DISTRIBUTION
Semen
10 % Formalin
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8.0 CALCULATIONS
( 1:20 )
Total number of semen counted = number of semen counted in 4 large squares
x 50,000
( 1:10 )
Total number of sperm = number of spermatozoa counted in 4 large squares x
100,000
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11.0 REFERENCE
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12.0 DISTRIBUTION
Approved by:
Dr. Mustafa Hameem
Director
Urine
Uritop + 10 Strips
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8.0 CALCULATIONS
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Chemical reactions are done using the Uritop Strips by comparing the color
thru the color chart as to their specific Gravity,pH,
Blood,Leukocytes,Glucose,Urobilinogen and Protein. Microscopic
examination is done under the low and high power objective for the Pus,
RBC,Epithelial cells, Casts, Crystals and other parasites.
11.0 REFERENCE
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12.0 DISTRIBUTION
Stool
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11.0 REFERENCE
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12.0 DISTRIBUTION
Reviewed by:
Mrs. Cherrielyn Gilay