BATCH

STERILIZATION
 TOPICS COVERED

Design of batch sterilization

Del factor
Kinetics of batch sterilization
DESIGN OF BATCH STERILIZATION PROCESS

Points to be considered before designing

a batch sterilization process-
• The profile of increase and decrease in
temperature during heating and cooling
periods.
• Total no. of microorganisms initially present in
media.
• Themal death characteristics of design organism.
It is less successful in avoiding the destruction of
nutrients than a continuous one. But still used with
minimum loss of nutritive quality.
Highest temp achieved in batch sterilization is 121ᵒ.
In this the contributions to sterilization during heating
and cooling peroids is considered.
 DEL FACTOR

It is the measure of fractional reduction in the

number of viable organisms over certain heat and
time regime. It is numerically :-

∇=ln (N0 /Nt )

where,
Nₒ= no.of viable organisms before sterilization
Nt = no. of viable organisms after sterilization time t.
Also, ∇ =kt
 KINETICS OF BATCH STERILIZATION

If the original no of organisms present (Nₒ) is known

and (Nt= 10-3) ,the required Del Factor can be
calculated.
For e.g: if a specific case in which unsterile broth was
shown to contain 1011 viable organism then the Del
factor may be calculated as-:
∇= ln (1011 / 10-3 )
= ln 1014
= 2.303 log 1014
∇ OVERALL = 32.242
Therefore the overall Dell Factor required is 32.2.
however, the destruction of cells occurs during the
heating and cooling of the broth as well as during the
period of 121ᵒC, thus the over all Del Factor may re
presented as:

∇ OVERALL = ∇ HEATING + ∇ HOLDING + ∇ COOLING

ADVANTAGES OF BATCH STERILIZATION OVER
CONTINUOUS STERILIZATION

Lower equipment cost

Lower chances of contamination
Easier manual control
THANK YOU