ANNASAHEB DANGE COLLEGE OF

ENGINEERING AND TECHNOLOGY,ASHTA

PROJECT TITLE
Design and Analysis of microchannel for plasma
Separation from blood
Presented by
1. Phalke Rushikesh pradip (862)
2. Shingate Nikhil Nandkumar (861)
3. Nikam Subodh Nivas(856)
4. Jankar Dipak Chandrakant (863)
5. Shinde Sourab Appaso (866)
6. Patil Parthesh Nandkumar (1161)

Under Guidance of
DR. A.B. Shinde
CONTENT
• INTRODUCTION
• OBJECTIVE
• LITERATURE STUDY
• MICRO CHANNEL MODELING STEPS
• RESULT AND DISCUSSION
• CONCLUSION
• REFERENCES
INTRODUCTION
• The separation of plasma from blood is an indispensable process in the domain
of disease diagnostics.
• So Here we seprate the plasma using microchannel by density effect i.e
Zweifach-fung law
• The simulation of separation of plasma from blood is done with COMSOL
Multiphysics software
• Where we discussed the various geomerty of microchannel like rectangle ,
traingle , ellipse etc. And We found that the rectangle geomerty gives us better
result
• Then we changing the parameters like flow rate, density, viscosity etc for the
getting more efficient result in rectangle geometry
OBJECTIVES

• Literature study
• To study different configuration of microchannel
• To carry out CFD analysis of micro-fluidic channel
• To validate the simulation result to Published data
 LITERATURE STUDY
• Conventional technologies for removal of blood component (cells) are centrifugation
and membrane filtration.
• New technologies like CD-like platform are complex and required expensive
instrumentations
• The plasma skimming effect exists naturally in microchannel.
• A bend in the microchannel induces a variation of the flow profile and results in an
increased width of the “cell-free layer”
• A sub-microchannel is placed downstream on the inner wall of the bend to collect the
plasma. It was observed that thinner is this sub-microchannel, better is this efficiency.
• Based on the principle that RBCs flowing through a bifurcating region of a capillary
blood vessel have a tendency to travel into the vessel which has a higher flow rate,.
proposed a blood plasma separation device in T-shape.
Micro Channel modelling steps
Selection of physics and study type

Write parameters

Create Geometry

Variables/performance characteristics

Thin film flow physics

Meshing

Study
Continued
Result
Model of microchannel
• In Model For separating we used
Bifurcation law

• At the beginning of microchannel

blood is supplied through opening in
the beginning . The microchannel
have 4 opening one is for inlet and
other 3 for outlet. Among the three
opening 2 are for plasma collection
and 1 for cells.

Fig 1: Rectangular model of microchannel

RESULT AND DISCUSSION
• Finalized Rectangular Model for plasma separation
• In that model we tested the particle trajectories Shown in figures
• Then again we changes the parameters for rectangle geometry for more
efficient result
• The plasma we can collect at opening made along the wall of
microchannel .
• The result we get are proven by previously published research papers.
Rectangular geometry with velocity and pressure

• In rectangular geometry
we test pressure and
velocity

• In velocity diagram the

velocity at axial
direction is high where
low at the upper and
lower wall of
microchannel

• And in the pressure

diagram pressure is high
at the inlet and low at
outlet of microchannel

Fig2 : Rectangular microchannel with pressure and veocity

Results for rectangular microchannel
Sr No FLOW
RATE VELOCITY (mm/s) PRESSURE (Pascale)
(mm/s)
axial Along beginni outlet
wall ng
1 0.5 0.65 0.15 0.65 0.15
2 0.6 0.72 0.1 0.7 0.09
3 0.7 0.8 0.08 0.9 0.1
4 0.8 0.9 0.09 1 0.16
5 0.9 0.9 0.2 1.1 0.2
6 1 1.3 0.3 0.3 0.15
Particle trajectories of rectangular microchannel
Graph for rectangular geometry

• First graph is flow rate vs velocity

where first plot shows the velocity at
axial direction. And second plot shows
the velocity at walls

• Second graph is flow rate vs pressure

where first plot shows the pressure at
inlet and second plot shows the
pressure at outlet

Fig 3- [1] graph of flow rate vs velocity

[2]graph of flow rate vs pressure
CONCLUSION
• Analyzing the components of blood is key diagnostic step in the detection of diseases and accurate
separation of plasma from blood cells plays from blood cells plays a crucial role in the precision of
lateral flow diagnostic test.
• Without using the whole blood for diagnostic the separation steps used to separate plasma minimize
the interference of cells in the process of analyte detection while improving the sensitivity and
selectivity of the assays in disease detection.
• As we seen in the pandemic , the people who get affected by covid-19 plasma therpy become the
life saviour for those people. As the plasma of recoverd patient having the antibodies present. The
plasma from blood of recovered patient is collected and transfer is to the affected patient .So by
only transferring plasma to patient because antibodies only present the plasma and remaing cells can
be transfer back to healthy person.
• Also these microchannel plasma separation technique as far more batter and superior than the
conventional plasma separation method like Centifugation, filteration.
• It is less costly as no chemical are involved in at and also it is environment friendly plasma
separation process.
 References
1. “Analysis of fluid separation in microfluidic T-channels”, Xiangdong Xue, Mayur K. Patel, Maïwenn Kersaudy Kerhoas, Marc P.Y.
Desmulliez, Chris Bailey, David Topham.Applied mathematical modelling, 2012

2 “Structural Design of Microfluidic Channels for Blood Plasma Separation”, Jingjing Zhang, Xueyong Wei, Xiangdong Xue, and
Zhuangde Jiang , Journal of Nanoscience and Nanotechnology Vol. 14, 1–8, 2014

3 “Effect of channel geometry on cell adhesion in microfluidic devices”, James V. Green, Tatiana Kniazeva, Mehdi Abedi, Darshan S.
Sokhey, Mohammad E. Taslimb, Shashi K. Murthy , Lab Chip, 2009, 9, 677–685.

4 “Enhanced blood plasma separation by modulation of inertial lift force”, Myung Gwon Leea, Joong Ho Shina, Sungyoung Choib, Je-
Kyun Parka. , Sensors and Actuators B 190 (2014) 311– 317

5 “Fast blood plasma separation device for point-of-care applications”, Pavol Durca, Frantisek Foret, Petr Kuban. ,talanta 183
(2018)55-60

6 “Hydrodynamic blood plasma separation in microfluidic channels”, Maiwenn Kersaudy-Kerhoas, Resham Dhariwal, Marc P. Y.
Desmulliez, Lionel Jouvet., Microfluid Nanofluid (2010) 8:105–114
 7 “Particle separation and sorting in microfluidic devices: a review”, P. Sajeesh, Ashis Kumar Sen, Microfluid
Nanofluid (2014) 17:1–52

8 “Performance study of microfluidic devices for blood plasma separation—a designer’s perspective”, Siddhartha
Tripathi, Y V Bala Varun Kumar, Amit Prabhakar, Suhas S Joshi and Amit Agrawal, Micromech. Microeng. 25 (2015)
084004.

9 “Study on different meander structured microchannel: a biofilter”, Praveen Kumar S, Ramesh R, Aravind T,
Biomedical Research 2017; 28 (8): 3688-3692.

10 “Capillary flow of blood in a microchannel with differential wetting for blood plasma separation and on-chip
glucose detection.”, Ashis kumar sen, M. Sneha Maria, P. E. Rakesh, T. S. Chandra, Article in Biomicrofluidics ·
September 2016;054108.