ANNASAHEB DANGE COLLEGE OF

ENGINEERING AND TECHNOLOGY,ASHTA

PROJECT TITLE
Design and Analysis of microchannel for plasma
Separation from blood
Presented by
1. Phalke Rushikesh pradip (861)
2. Shingate Nikhil Nandkumar (860)
3. Nikam Subodh Nivas(855)
4. Jankar Dipak Chandrakant (862)
5. Shinde Sourab Appaso (865)
6. Patil Parthesh Nandkumar (1161)

Under Guidance of
DR. A.B. Shinde
CONTENT:
 INTRODUCTION
 OBJECTIVE
 MICRO CHANNEL MODELING STEPS
 RESULT AND DISCUSSION
 CONCLUSION
 REFERENCES
1. INTRODUCTION:
Microfluidics has developed as a set of powerful tools that have greatly
advanced in some areas of biological research. The use of microfluidics has enabled many
experiments that are otherwise impossible with conventional methods and is highly capable of
providing advantages in terms of a significant reduction in the sample processing time, sample
volumes, and consumption of costly reagents. Various research groups have established
continuous flow separation methods in microfluidic devices; however, they have worked with
relatively small dimension microchannels .
Our work demonstrates separation of plasma by utilizing the hydrodynamic
separation techniques in microchannels with size of the order of mm. The separation process uses
the phenomenon, which is very similar to Zweifach-Fung bifurcation law. The device working
principle based on density effect. The rectangular microchannel device simulation we made to
understand the how this gonna work. While designing the microchannel three variables are
important main channel width, flow rate and manufacturing technique used to make the
microchannel.
2. OBJECTIVES:
1. Literature study
2. To study different configuration of microchannel
3. To carry out CFD analysis of micro-fluidic channel
4. To validate the simulation result to Published data
Following are the expected outcomes:
1. Testing the various blood components acc. to medical prescriptions,Each blood component has its
unique role and function, thus separating it is the crucial process of the examination
2. simplicity of use and rapid results obtained,
3. The presented filter microchannel can be used to separate plasma for different plasma testing.
3.Micro Channel modelling steps
Selection of physics and study type

Write parameters

Create Geometry

Variables/performance characteristics

Meshing

Study

Result
 CFD ANALYSIS FOR MICROCHANNEL MODELLING:

1. Selection of physics and study type:

 For our model we tend to use 2d geometry then we choose 2 physics 1st one is creeping flow and second is particle
tracing for fluid flow. The creeping flow is employed for simulating fluid flows.
 In that single-phase flow kind is acts as Stoker’s flow and happens in systems with high body or tiny geometrical
length scales .The fluid will be compressible or incompressible, and Newtonian or non-Newtonian. The equations
resolved by the creeping Flow interface area unit the Stokes equations for conservation of momentum

 and therefore the continuity equation for conservation of mass. The creeping Flow interface will be used for
stationary and time-dependent analysis.

 The particle tracing for fluid flow is applied for the Particle the motion of particles in an exceedingly
background fluid. Particle motion will be driven by drag, gravity, and electrical, magnetic, and
acoustophoretic forces. .
2. Add parameter:

Parameters given to software.

 3. Create geometry:
In model we create 2D geometry having length 2000micron and height 600 micron. The model has 1 inlet and 3
outlet out of them 2 outlet for plasma separation and one for cell Collection. It has 5 set obstacle inside.

4. Meshing:
For meshing we use physics control mesh which is fine meshing. Finer the mesh more the accuracy.

5. Study:
For study we add two study to the component. First study for stationary flow and second for the time dependent.
 RESULTS:
For microchannel modelling and simulation we designed different geometries like rectangle, semicircle, circle, triangle
and ellipse. Also we check different result for different flow rate, particle size, density. Among all the result we get
some satisfactory results which are similar to previously published research paper.
1. Different geometry Configuration:
1.1Rectangle:

Rectangle Geometry
1.2Square: Semi-circle:

Square geometry Semicircle geometry

Triangular geometry
Results And Analysis :
1 Triangle:
a. Geometry: This is the triangular geometry in that the 5 triangles are there. This geometry have one inlet for blood entry
and the three outlets, two of them for the plasma and centre one for the other blood components

Mesh: Triangular geometry

Meshing of triangular geometry

C. Velocity: We check different velocity effect on triangular microchannel from 0.5mm/sec to 1mm/sec .As model obey
Bifurcation law the different velocity regions created inside the microchannel. But in this geometry vortex is not created
properly as compared to rectangular geometry. So the axial region having high velocity and outer boundary having low
velocity represent with red in colour.

0.5mm/sec:

Velocity magnitude in triangular

e. Particle trajectory:

Particle trajectory in Triangular

The above figure consisting of triangular geometry microchannel for plasma separation from blood. It has one inlet
through which blood is entering and three outlet in which two extreme side outlets are for the plasma and centre outlet is
for remaining blood components.
Also it having the 4 rectangles in that geometry. In that the efficiency of plasma separation is low as compared to
rectangular microchannel cause the vortex not crate properly in this geometry.
 2.Semi-circle:

Geometry: This is the geometry of the semi-circular microchannel. It has the 5 semi-circles. Also this geometry have the
one inlet and three outlets.

Semicircle geometry

Mesh:

Meshing Of semicircle geometry

Velocity:
Surface: This diagram representing the semi-circular geometry of microchannel along with the velocity magnitude. In that
geometry the blood enters through inlet and strike on the curved surface and create the vortex but vortex is not proper due to
that plasma separation efficiency is low. And in this figure the velocity of blood at the middle of microchannel is high.

Velocity magnitude in semicircle

Arrow surface:

This diagram shows the separation effect by using arrows. Means how blood enters in microchannel and how
vortex created and how separate the plasma and other blood components such as RBC's WBC's and platelets etc.

Arrow surface in semicircle

Pressure:
This diagram represents the pressure contour in semi-circular geometry with pressure in Pascal at various point in the
microchannel. As in fig the different colours are shown that means the how much pressure at inlet, at middle and outlet. At
the inlet the colour is red so the pressure of blood is high and going out the pressure drops mean the colour changes and at the
outlet the pressure is low

Pressure in semicircle
Particle trajectory:

Particle trajectory in semicircle

3.Square:
Geometry:
This is the geometry of square microchannel. It has the 8 square obstacles in it like the rectangular geometry. And same as
the other geometries having one inlet and 3 outlets.

Mesh:

Square geometry

meshing of square geometry.

Velocity:
This diagram representing the square geometry of microchannel along with the velocity magnitude. In that geometry the blood
enters through inlet and strike on the square obstacles and create the vortex but vortex is not proper as compared to rectangular
geometry vortex due to that plasma separation efficiency is low. And in this figure the velocity of blood at the middle of
microchannel is high.

Velocity magnitude in square

This diagram shows the separation effect by using arrows. Means how blood enters in microchannel and how vortex
created and how separate the plasma and other blood components such as RBC's WBC's and platelets etc.

Arrow surface in square

Pressure: This diagram represents the pressure contour in square geometry with pressure in Pascal at various point in the
microchannel. As in fig the different colours are shown that means the how much pressure at inlet, at middle and outlet. At
the inlet the colour is red so the pressure of blood is high and going out the pressure drops mean the colour changes and at
the outlet the pressure is low.

Pressure in square
Particle trajectory:
In this square microchannel we observed that when the fluid enter inside the channel it flow forward and collide on square
obstacles due velocity vortex is created near boundary of curved surface. But the vortex is not so good due to that all the
blood particles are going through only the centre. And not separate the plasma. So the bifurcation law is not work properly in
this geometry. We also teste this study for 5 different velocities from 0.5 to 1 mm/sec.
 4 Rectangle:
1. Geometry:

These the rectangular microchannel geometry having one inlet and three outlets. These contain five pairs of
rectangular shaped obstacles. Which helps to divide the flow and separate blood components.

2 Mesh:
These mesh contains 12158 elements for getting accurate
result in meshing we done corner refining and sizing of
elements.
3 Velocity:
We check different velocity effect on microchannel from 0.5mm/sec to 1mm/sec.As model obey Bifurcation law the
different velocity regions created inside the microchannel. The axial region having high velocity and outer boundary having
low velocity.
0.5mm/sec:

For inlet velocity of 0.5mm/sec we get maximum output of 0.71mm/sec.

Arrow surface:

As blood flow inside channel it collide on obstacle. It result in creating vortex near the edges of obstacles the vortex create
pressure difference inside the channel hence dense particles of blood settle down and move along high velocity channel
which is in axial direction and low density components move alongside the wall.
0.6mm/sec:

0.7mm/sec:
With higher velocity the vortex are get bigger and bring the low density particles in high velocity channel which is not good.

0.8mm/sec: 0.9mm/sec:
1mm/sec:

Pressure in rectangle

Arrow surface in rectangle

With increase in velocity the particle disturbance is increase.
Inlet vs outlet velocity Comparison:
Inlet velocity mm/sec Outlet velocity mm/sec
0.5 0.71
0.6 0.85
0.7 0.99
0.8 1.13
0.9 1.27
1 1.42

Inlet vs outlet velocity Comparison in rectangle channel

Inlet velocity for channel with rectangular obstacles

3. Pressure:
0.5mm/sec:

0.6mm/sec:
0.7mm/sec:

0.8mm/sec:
0.9mm/sec:

1mm/sec:

Fig 14 Pressure in rectangle.

Inlet pressure vs outlet pressure comparison:
Inlet pressure Pa outlet pressure Pa
0.5 0.79
0.6 0.95
0.7 1.1
0.8 1.26
0.9 1.41
1 1.58
Inlet pressure vs outlet pressure comparison in rectangle channel

Inlet velocity for channel with rectangular obstacles

Particle trajectory:

16 Particle trajectory in rectangle.

In rectangular microchannel we observed that when the fluid enter inside the channel it flow forward and collide on
rectangular obstacle due velocity vortex is created near boundary of obstacle.

The fluid rotates around the obstacle and particles get separated due to density effect. The high density fluid tends to move
along the axial direction and low density fluid along the wall.

These behaviour is similar to that of bifurcation law. Which states that high density fluid such as WBC, RBC are move along
the axial direction and can be separated plasma and cell can collected at outlet of channel.

As plasma having low density it move along the wall of microchannel and it can be collected at 2 opening provided along the
walls of channels.

For our model we get satisfactory results for velocity inlet 0.5 mm/sec. As we increase the velocity the particles collide on
each other and does separate they mix with each other.
7. CONCLUSION
 Analyzing the components of blood is key diagnostic step in the detection of diseases and accurate
separation of plasma from blood cells plays from blood cells plays a crucial role in the precision of
lateral flow diagnostic test.
 Without using the whole blood for diagnostic the separation steps used to separate plasma minimize
the interference of cells in the process of analyte detection while improving the sensitivity and
selectivity of the assays in disease detection.
 As we seen in the pandemic , the people who get affected by covid-19 plasma therpy become the life
saviour for those people. As the plasma of recoverd patient having the antibodies present. The plasma
from blood of recovered patient is collected and transfer is to the affected patient .So by only
transferring plasma to patient because antibodies only present the plasma and remaing cells can be
transfer back to healthy person.
 Also these microchannel plasma separation technique as far more batter and superior than the
conventional plasma separation method like Centifugation, filteration.
 It is less costly as no chemical are involved in at and also it is environment friendly plasma separation
process.
 References

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Thank You