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  • SG RNA

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    liuk199703086084
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    The document provides information for generating clones containing CRISPR guide RNA sequences targeting various regions of several NAC genes. It lists the name and genomic location targeted for 12 different guide RNAs, along with the corresponding forward and reverse oligonucleotide sequences and the vector into which each guide will be cloned.

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    sgRNA

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    The document provides information for generating clones containing CRISPR guide RNA sequences targeting various regions of several NAC genes. It lists the name and genomic location targeted for 12 different guide RNAs, along with the corresponding forward and reverse oligonucleotide sequences and the vector into which each guide will be cloned.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    3 views2 pages

    SG RNA

    Uploaded by

    liuk199703086084
    The document provides information for generating clones containing CRISPR guide RNA sequences targeting various regions of several NAC genes. It lists the name and genomic location targeted for 12 different guide RNAs, along with the corresponding forward and reverse oligonucleotide sequences and the vector into which each guide will be cloned.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    Generate pIK5 and pIK6 clones

    gRNA Forward Reverse Vector


    NAC9 1 NAC9guide34for NAC9guide34rev
    -34 CTTGGACAAGGCGATCCTCCTACCGG AAACCCGGTAGGAGGATCGCCTTGTC pIK5
    NAC9 2 NAC9guide82for NAC9guide82rev
    -82 CTTGGAAGACGCATTAATGGTGGCGG AAACCCGCCACCATTAATGCGTCTTCC pIK6
    NAC1 3 NAC17guide65for NAC17guide65rev
    7-65 CTTGGCCCGGTTGGTCCGTACTCCGG AAACCCGGAGTACGGACCAACCGGGCC pIK5
    NAC1 4 NAC17guide7for NAC17guide7rev
    7-7 CTTGAAGAAACCCTTCGACACGATGG AAACCCATCGTGTCGAAGGGTTTCTTC pIK6
    NAC1 5 NAC18guide49for NAC18guide49rev
    8-49 CTTGGTGGAGGCTTTCTCTGCAATGG AAACCCATTGCAGAGAAAGCCTCCACC pIK5
    NAC1 6 NAC18guide28for NAC18guide28rev
    8-28 CTTGATACGAACTAACCGGGCGACGG AAACCCGTCGCCCGGTTAGTTCGTATC pIK6
    NAC1 7 NAC19guide1for NAC19guide1rev
    9-1 CTTGCAGCAACAACAACAACAGTTGG AAACCCAACTGTTGTTGTTGTTGCTGC pIK5
    NAC1 8 NAC19guide29for NAC19guide29rev
    9-29 CTTGGTTGGCTACTGGAAGGCCACGG AAACCCGTGGCCTTCCAGTAGCCAACC pIK6
    NAC4 9 NAC410guide9for NAC410guide9rev
    10-9 CTTGAAGAAACCCTTCGACACCAGGG AAACCCCTGGTGTCGAAGGGTTTCTTC pIK5
    NAC4 10 NAC410guide62for NAC410guide62rev
    10-62 CTTGGCTCTGTTAGTCCGTATCCCGG AAACCCGGGATACGGACTAACAGAGCC pIK6
    NAC2 11 NAC24guide76for NAC24guide76rev
    4-76 CTTGGCCCGGTTGGTCCGTATACCGG AAACCCGGTATACGGACCAACCGGGCC pIK5
    NAC2 12 NAC24guide95for NAC24guide95rev
    4-95 CTTGGAAGATGCGTTAATGGCGGCGG AAACCCGCCGCCATTAACGCATCTTCC pIK6

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