Anhui Polytechnic University

Single cell ‘Glucose Nanosensor verifies elevated

glucose levels in individual cancer

王军 | ELMALIH HASSANE
E-mail:Malihhassan1998@gmail.com

2023/2024
 Single cell ‘Glucose Nanosensor’
verifies elevated glucose levels in
individual cancer

Raphael A.S. Nascimento, Rifat Emrah Ozel, Wai Han Mak,

Marcelo Mulato, Bakthan Singaram, and Nader Pourmand
Nano Lett., Just Accepted Manuscript • DOI: 10.1021/acs.nanolett.5b04495 • Publication
Date (Web): 11 Jan 2016
Downloaded from http://pubs.acs.org on January 13, 2016
Corresponding author

Nader Pourmand
Institution
University of California, Santa Cruz
Department
Department of Biomolecular Engineering
Last publication
High throughput isolation of RNA from single-cells within an intact tissue for spatial and temporal sequencing
a reality.
Resrarch area

Bioelectronics, Biosensors, Chemosensors, DNA fingerprinting, Single cell characterization,

Nanotechnology.
 Abstract

 Relationship between glucose

metabolic and cancer cell.

 Glucose metabolic on cancer cell.

 Secondary effect.

 Hallmark.
 Introduction

 Single-cell approach.

 Principle.

 Method.
Material and Method
 Materiel
Nanopipette
Nanopipettes are pipettes in nanometer scale, generally
made from quartz capillaries with the help of laser-based
pipette puller system.
 Method
Result and discussion

Figure 1: Single cell surface finding and intercellular glucose using glucose
nanosensor.
Figure 2: A- nanopipette in the absence of Gox and B-nanpipette in the present Gox
Figure 3: measured intracellular glucose levels of thecell lines
Figure 4: Comparison of intracellular glucose levels
CONCLUSION

Our technology can therefore be applied to

diagnostic and mechanistic studies of cancer, and
would also be applicable to other investigations of
metabolic heterogeneity in a cell population.
 References

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