A comprehensive review on the detection of various bio-analytes using

Electrochemiluminescence:
Parthesh Patil Samruddhi Rahangdale Suyog Vyas Saniya Pajare
Siddhi Tiwari
Mentor : Dr Manish Bhaiyya sir
Department of Biomedical Engineering
1. Background And Motivation: 2. Introduction to ECL:
Electrochemiluminescence (ECL) combines electrochemical reactions
and luminescence, converting electrical energy to light. It is made of three
words :

Electro: electrical stimulation

Chemi: chemical reaction

Luminicense: light

ECL reagents : Luminol/H202 and Ruthenium/TpRa

3. Advantages of using ECL technology: 4. ECL Systems Classification

1 highly sensitive and specific ECL

SYSTEM
Advantages of affordable and user friendly
2
using ECL BIPOLAR E. SINGLE E.
technology for 3 rapid and robust ECL ECL
bio-analyte
detection 4 minimal background signal

5 broad dynamic range OPEN BPE CLOSE BPE

5. ECL APPLICATIONS : 6.ECL vs other conventional methods:

Drug Screening and pharmacology
Genetic analysis ECL
Point of care testing devices
ECL VS PHOTO-LUMINESCENCE:
No external light source required
High signal to noise ratio
ECL vs CHEMI-LUMINESCENCE:
Greater selectivity and sensitivity as intensity
Immunoassay
can be controlled by varing potential
ECL vs ElECTROCHEMICAL:
More accuracy and precision, less elecrtrode
fouling

5. Conclusion : 6. Reference

1. Richter MM: Electrochemiluminescence ( ECL ). Opt

Thus a comprehensive review on the detection of various bio- Biosensors 2004:317–384, https://doi.org/10.1016/B978-
044453125-4.50009-7. Elsevier
analytes using ECL was made .
2. Gao W, Saqib M, Qi L, Zhang W, Xu G: Recent advances in
electrochemiluminescence devices for point-of-care testing. Curr
Opin Electrochem. 2017, 3:4–10, https://doi.org/10.1016/
j.coelec.2017.03.003.

3. Miao W: Electrogenerated chemiluminescence and its bio related

applications. Chem Rev 2008, 108:2506–2553, https://
doi.org/10.1021/cr068083a.