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Control
When
Both
Cont.
When
Reciprocal
1.
Fructose-6-phosphate
Fructose-1,6-bisphosphate
Glycolysis- Red Gluconeogenesis- yellow
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2.
Phosphoenol pyruvate Oxaloacetate pyruvate
Cont.
Pyruvate
The
The
At
Gluconeogenesis (+) Pyruvate carboxylase Pyruvate (-) pyruvate dhase Acetyl- CoA
When
NADH NADH
High
High
Acetyl
CoA also acts as a biosynthetic precursor. Acetyl CoA concentration inhibits pyruvate dehydrogenase Acetyl CoA also stimulates pyruvate carboxylase
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Increased
Increased
Increased
Pyruvate
Pyruvate
Pyruvate
ADP
When
ATP levels are falling, and ADP levels are rising, the ADP inhibits pyruvate carboxylase so more pyruvate will be channelled into citric acid cycle.
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The
ADP
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Phosphoenol pyruvate PK
Oxaloacetate pyruvate
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Cont.
Pyruvate kinase (PK) catalyses conversion of PEP to pyruvate in glycolysis PK is inhibited when energy levles are high and stimulated when energy levles are low
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bisPase) reaction.
This
F-1,6 bisPase
Fructose-1,6-biphosphate Pyruvate
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The
It
It
The
It
and gluconeogenesis are adjusted in the liver to maintain blood glucose. blood glucose levels decrease, glucagon rises.
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When
Adenyl
cyclase stimulates formation of 35 cyclic AMP from ATP stimulates cAMP dependent protein Kinase A
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cAMP
Protein
The
bifunctional protein is made of one enzyme at one end and another enzyme at its other end.
The
Fructobiphos phatase-2
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The
protein Kinase A phosphorylates a serine residue on the bifunctional enzyme and this leads to activation of F biPase-2 and the inhibition of PFK-2
of F biPase-2 leads to breakdown of Fructose-2,6bisphosphate (F-2,6-biP) and a decreased levels of F-2,6-biP
Activation
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Low
At
the same time low levels of F2,6-P will stimulate F-1,6-biPase and gluconeogenesis
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glucagon
cAMP protein Kinase A
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The
control by glucagon also relies on the fact that F-2,6-bP stimulates PFK
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PEP
F-1,6-bPase
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F-6-P F-1,6-bPase
F-1,6-bP
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F-6-P
The
This
Unlike
Conversion
Control
G-6-Pase
is only present in Liver and Kidney- so only these organs can release glucose into the blood. of G-6-P into glucose takes place in the Lumen of endoplasmic reticulum (ER)
Conversion
Gluconeogenesis
cytoplasm.
The
In
G-6-Pase
Glucose
Vigorous
exercise can lead to oxygen shortage (anaerobic conditions), and energy requirements must be met by increased levels of glycolysis. Under such conditions, glycolysis converts NAD to NADH, yet O2 is unavailable for regeneration of
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Under
such conditions, glycolysis converts NAD to NADH, yet O2 is unavailable for regeneration of NAD via cellular respiration.
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Instead, large amounts of NADH are reoxidized by the reduction of pyruvate to lactate. The lactate thus produced can be transported from muscle to the liver, where it is reoxidized by liver lactate dehydrogenase to yield pyruvate, which is converted eventually to glucose.
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In this way, the liver shares in the metabolic stress created by vigorous exercise. It exports glucose to muscle, which produces lactate, which can be processed by the liver into new glucose.
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This
is referred to as the Cori cycle. Liver, with a typically high NAD/NADH ratio (about 700), readily produces more glucose than it can use.
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Muscle
that is vigorously exercising will enter anaerobiosis and show a decreasing NAD/NADH ratio, which favours reduction of pyruvate to lactate.
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Glyoxylate Cylce
Occurs
Involves
The
The
PEP can be used to form glucose in gluconeogenesis. means that organisms which have the glyoxylate cycle can use acetyl CoA as a starting material for gluconeogenesis. do not have the glyoxylate
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This
Vertebrates
cycle.
Acetyl
CoA cannot be converted into pyruvate because the following reaction is irreversible: Pyruvate
pyruvate dehydrogenase complex Acetyl CoA
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There
is no NET conversion of acetyl CoA into oxaloacetate in the Citric acid cycle because;
for every 2 carbons that enter as Acetyl CoA, 2 carbons leave as Carbon dioxide.
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