X

10-510-3nM
10-40.1nM
10 20nM
200400nM
400760nM
0.7650M
501000M
0.1100cM
1100M

2.



X X

3.()

>0.35

Stokes shift

Stokes shift

hvEX hvEM =

4.
1

>

Excitation Spectrum

Emission Spectrum

EX
Wavelength

EM

EX

EM


1838

Brewster

1852

Stokes

1903

Wood

1911

Reicherl

1935

Haitinger

JC-1, Rhodamine 123

DAMPNeutral Red
Dioc6(3),Dioc53,Dioc16(3)

NBD Ceramide, BODIPY Ceramide

.
F- BONIPY FL, BONIPY 558/568
G-DNase I+

Alexa Fluor 488

Oregon Green 488
Texas Red

+
DAPI
DCVJ
Bis-ANS
Flutax-2
BODIPY FL Placlitaxel
BODIPY 564/570
Placlitaxel

PKCBODIPYA

Quin-2-AM

Fura-2-AM
Fura-3-AM

.
DNA/RNADNA/RNA

DNA

SYTO
AO
Hoechst
DAPI
EBPI
PO-PROBO-PRO
AAD
ACMA

GFP
1.GFP60Shimomura
(aequoren)

Ca2+
Aequoren +

green fluorescent protein GFP

GFP

GFP
GFP
1.GFP 460-490 nm

500-550 nm

2. GFP20kDa

GFP
1.
2.
3.
4.GFP
5.

GFP

GFP, CFP, BFP, YFP

1. : Green mouse
2. : GFP DNA ,
.
3. :
GFP DNA , .
4. GFP-: GFP DNA


1.

ul,ml)

2.

3.FCM

4.

KCLCa++
KCL

KCL