"SOLID PHASE EXTRACTION"

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"A"

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"SPE3"

SPE is necessary to remove interfering matrix constituents such as proteins and quantify to pg/mL levels, particularly for metabolites and bioactive drugs.

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"strategy2"

The first strategy is usually chosen when the desired sample component is present in high concentration. When components of interest are present at low levels, or multiple components of widely differing polarities need to be isolated, the second strategy is generally employed. The second strategy may also be used for trace enrichment of extremely low level compounds and concentration of dilute sample. A complex matrix may be treated by both elution strategies to isolate different target analytes.

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"spe1"

SPE is typically performed by loading the complex sample onto a preconditioned extraction cartridge containing a chromatographic sorbent.

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"rack1"

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"elution1"

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"Three major purposes"

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"np1"

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"rp1"

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"ie1"

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"Chromatographic Modes and Sorbent Types "

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"ease1"

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"consider1"

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"ideal spe1"

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"Stationary Phase Supports"

40

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"Solid Phase Extraction"

45

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"Comparison of Efficiency - HPLC vs. SPE"

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" Calculate maximum surface coverage on C18 Sep-Pak Mass sorbent/cartridge "

46

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"Differences between HPLC and SPE"

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"SPE Strategies"

11

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"Common Problems in SPE"

12

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"SPE procedure"

13

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"Unnamed"

q q

14 tep 1 - Sample Preparation

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"Unnamed"

15

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"Step 2 - Sample load "

16

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"Step 2 - Sample Load"

22

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"Step 3 - Wash"

23

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"Step 3 - Wash"

24

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"Step 4 - Elute"

25

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"Step 4 - Elution"

33

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"The Silica Surface Silanophilic Interactions"

27

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"Mixed Mode Effects"

28

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"incomplete"

41

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"Low Recovery"

42

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"variability"

43

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"Conclusions"

44

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"Conclusions"

44

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