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    Georgi Tonarelli
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    Chemical Ionization (CI) Mass Spectrometry (CI-MS) as a chemical reactor 1950 1960 year Ionization method : (combined) : Molecular mass 500 range : analyte amount : target analyte : mg-g EI LD CI API FAB FI PI FD PD SIMS GC / MS 300,000 fg Biopolymers proteins (low -high) glycoproteins

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    EM-2-09

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    Chemical Ionization (CI) Mass Spectrometry (CI-MS) as a chemical reactor 1950 1960 year Ionization method : (combined) : Molecular mass 500 range : analyte amount : target analyte : mg-g EI LD CI API FAB FI PI FD PD SIMS GC / MS 300,000 fg Biopolymers proteins (low -high) glycoproteins

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    11 views20 pages

    Em 2 09

    Uploaded by

    Georgi Tonarelli
    Chemical Ionization (CI) Mass Spectrometry (CI-MS) as a chemical reactor 1950 1960 year Ionization method : (combined) : Molecular mass 500 range : analyte amount : target analyte : mg-g EI LD CI API FAB FI PI FD PD SIMS GC / MS 300,000 fg Biopolymers proteins (low -high) glycoproteins

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    of 20

    What is Chemical Ionization (CI) Mass Spectrometry (CI-MS)

    Bombing gas solid/liquid Ag

    MH+ [Ag+H]+

    Heat !!

    AP (NO vacuum) !!!

    Chemical Ionization (CI) Mass Spectrometry (CI-MS) as a chemical reactor

    EI-MS

    EI-MS of undecanol; (a) 7 eV, (b) 12 eV

    EI-MS and CI-MS

    CI-MS

    EI-MS and CI-MS

    CI-MS (gas: CH4, iso-C4H10, NH3)

    EI-MS and CI-MS ((a) EI-MS; (b) and (c) CI-MS, gas: CH4, iso-C4H10)

    1950 1960 year Ionization method : (combined) : Molecular mass <500 range : analyte amount : target analyte : biological science : mg-g

    1970

    1980

    1990

    2000

    EI LD CI API FAB FI PI FD PD SIMS GC/MS

    UV-MALDI
    ESI

    LC/MS

    <1000 ng

    <5000 pg

    <300,000 fg Biopolymers proteins (low high) carbohydrates proteomics bio biochemistry environment

    ? ?
    glycoproteins glycoconjugates glycomics Agriculture (plant) medical treatment (animal) Mixture Cell

    (low mass, thermostabilized, volatilized compounds)

    Industrial Steel petroleum usage : Pharmacy (oil)

    medicine semiconductor

    Mass Spectrometry development

    What is Field Ionization (FI) Mass Spectrometry (FI-MS)

    What is Field Ionization (FI) Mass Spectrometry (FI-MS) FI-MS

    EI-MS vs FI-MS

    A
    Analyte

    Volatilization /Ionization

    A
    gas-ion

    +
    Analysis vacuum

    RI

    m/z

    vacuum
    bombardment
    H

    e-

    A+ A-

    A: gas analyte A+: gas analite cation A-: gas analyte anion H: proton e-: electron

    A A

    A+
    H

    A m A
    A

    A A A

    Solid sample

    Electrode

    Electrode (+)

    What is DESORPTION ? (FD; PD; LD; FAB; LSIMS; SIMS; MALDI-MS)

    REB

    What is Desorption (volatilization)?

    10

    Spinodal

    11

    Ablation (images LM polymer + GA, Fatema 0802; UVMALDI)

    12

    Femtosecond laser irradiation ablation(toluene)


    Masuhara et al. (Oaska Univ.)J.Phys.Chem. B, 106, 3049(02)

    Time Dependence of the Femtosecond Laser Ablation of Liquid Toluene Surface


    Ti3+;:Al2O3,/KrF (248 nm, 300-500 fs)

    J. Phys. Chem. Vol.106, 3049 (2002) :Masuhara et al.

    delayed extraction

    13

    What is Field Desorption (FD) Mass Spectrometry FD-MS ?

    What is Field Ionization (FI) Mass Spectrometry (FI-MS) and Field Desorption (FD) Mass Spectrometry (FD-MS)

    HEAT High pressure Vacuum

    14

    FD-MS

    1950 1960 year Ionization method : (combined) : Molecular mass <500 range : analyte amount : target analyte : biological science : mg-g

    1970

    1980

    1990

    2000

    EI LD CI API FAB FI PI FD PD SIMS GC/MS

    UV-MALDI
    ESI

    LC/MS

    <1000 ng

    <5000 pg

    <300,000 fg Biopolymers proteins (low high) carbohydrates proteomics bio biochemistry environment

    ? ?
    glycoproteins glycoconjugates glycomics Agriculture (plant) medical treatment (animal) Mixture Cell

    (low mass, thermostabilized, volatilized compounds)

    Industrial Steel petroleum usage : Pharmacy (oil)

    medicine semiconductor

    Mass Spectrometry development

    15

    What is Fast Atom Bombardment (FAB) Mass Spectrometry (FAB-MS) ? What is Liquid Secondary Ions (LSI) Mass Spectrometry (LSIMS) or Secondary Ions (SI) Mass Spectrometry (SIMS)?

    16

    What is Fast Atom Bombardment (FAB) Mass Spectrometry (FAB-MS) ?

    17

    FAB vs FAB + Fragmentation

    What is Liquid Secondary Ionization (LSI) Mass Spectrometry (LSIMS or SIMS)

    18

    SIMS: different energetic ionic gas particles used for imaging

    SIMS: examples and appIications

    19

    SIMS&Cluster SIMS
    Ga+: R 1 m
    0ps 1ps
    Mixing of layers

    7.5ps

    29ps

    Ag{111}

    Low desorption efficiency, damage of the sample inside

    Cluster SIMS C60+ : R 5 m

    High momentum collision Ag{111}

    Mixing of layers

    High desorption efficiencyhigh Z. Postawa et. al. J. Phys. Chem. B 2004, 108, 7831-7838 sensitive
    craterSIMS

    20

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