Data

Table 1: Sample Preperation

Dilution Buffer
Dilution Factor
Volume mL
Influent
90
10
99
100
99
10,000
100
10,000,000
2nd Effluent
0
1
99
100
99
10,000
2nd Effluent (Disinfected)
0
1
99
100
Tap Water
0
1
90
10
Dilution Buffer
100
1

Sample

Sample Volume mL

Number of Bottles

A
B
C
D

10
1
1 (from B)
0.1 (from C)

x2
x2
x2
x2

A
B
C

100
1
1 (from B)

A
B

100
1

A
B

100
10

x2
x2
x2
x2
x2
x2
x2
x2

Table 2: HPC Raw Data

Number of Colonies on Plate
Rep. No. 1
Rep. No. 2
Too many
356
240
248
Cannot Count
60
2
0
Too many
Too Many
590
468
185
9

Sample ID

Dilution Factor

Sample Volume, mL

Influent A
Influent B
Influent C
Influent D
2nd Effluent A
2nd Effluent B
2nd Effluent C
2nd Effluent
(Disinfected) A
2nd Effluent
(Disinfected) B
Tap Water A
Tap Water B
Dilution Buffer
Control

10
100
1000
107
1
100
1000

0.1
0.1
0.1
0.1
0.1
0.1
0.1

0.1

Too Many + Mold

Too Many + Mold

100

0.1

1
10

0.1
0.1

644
51

600
61

0.1

Agar (closed control)

n/a

0.1

Agar (open control)

n/a

0.1

Results
Influent A

Dilution
Factor
10

Influent B

100

Sample ID

Influent C

10,000
7

Plate Dilution
10-1 x 0.1mL
10-2 x 0.1mL

No. of Colonies on Plate

Rep. No. 1
Rep. No. 2
Too many
356

CFU/mL
Rep. No. 1
Rep. No. 2
--35,600

240

248

240,000

248,000

-4

Cannot Count

60

---

6,000,000

-7

2
Too many
590

0
Too Many
468

185
Too Many +
Mold

9
Too Many +
Mold

18,500,000

900,000

---

---

10 x 0.1mL

Influent D
2nd Effluent A
2nd Effluent B

10
1
100

10-2 x 0.1mL

2nd Effluent C
2nd Effluent
(Disinfected) A
2nd Effluent
(Disinfected) B
Tap Water A
Tap Water B
Dilution Buffer
Control
Agar (closed
control)
Agar (open
control)

10,000

10-4 x 0.1mL

1 x 0.1mL

100

10-2 x 0.1mL

1,000

2,000

1
10

1 x 0.1mL
10 x 0.1mL

644
51

600
61

6,440
5,100

6,000
6,100

1 x 0.1mL

10

<10

n/a

n/a

Sample ID
Influent A
Influent B
Influent C
Influent D
2nd Effluent A
2nd Effluent B
2nd Effluent C
2nd Effluent
(Disinfected) A
2nd Effluent
(Disinfected) B
Tap Water A
Tap Water B
Dilution Buffer
Control
Agar (closed
control)
Agar (open
control)

10 x 0.1mL
1 x 0.1mL

-2

Dilution li Large Wells Pos Ecoli Small Wells Positive

Factor
Rep. No. 1
Rep. No. 1
Rep. No. 2
10
48
48
48
100
48
48
48
1000
48
26
29
7
1
1
0
10
1
48
48
48
100
41
13
13
1000
5
0
0

200,000,000 <100,000,000
----590,000
468,000

MPN/100mL
Rep. No. 1
Rep. No. 2
1011.2
1011.2
1011.2
1011.2
360.9
416
2
1
1011.2
1011.2
113
113
5.2
4.1

100

<1

<1

<1

<1

1
10

0
0

0
0

0
0

<1
<1

<1
<1

<1

<1

n/a

<1

<1

n/a

<1

<1

Sample ID
Influent A
Influent B
Influent C
Influent D
2nd Effluent A
2nd Effluent B
2nd Effluent C
2nd Effluent
(Disinfected) A
2nd Effluent
(Disinfected) B
Tap Water A
Tap Water B
Dilution Buffer
Control
Agar (closed
control)
Agar (open
control)

Sample ID
Influent A
Influent B
Influent C
Influent D
2nd Effluent A
2nd Effluent B
2nd Effluent C
2nd Effluent
(Disinfected) A
2nd Effluent
(Disinfected) B
Tap Water A
Tap Water B
Dilution Buffer
Control
Agar (closed
control)
Agar (open
control)

TC Large Wells Positive

Rep. No. 1
Rep. No. 2
48
48
48
48
48
48
2
5
48
48
48
48
8
6

TC Small Wells Positive

Rep. No. 1
Rep. No. 2
48
48
48
48
47
40
1
0
48
48
26
25
1
0

MPN/100mL
Rep. No. 1 Rep. No. 2
1011.2
1011.2
1011.2
1011.2
960
689.3
3
5.2
1011.2
1011.2
360.9
344.1
8.7
6.3

<1

<1

<1

<1

0
0

0
0

0
0

0
0

<1
<1

<1
<1

<1

<1

<1

<1

<1

<1

CFU/mL
Rep. No. 1
Rep. No. 2
--35,600
240,000
248,000
--6,000,000
200,000,000 <100,000,000
----590,000
468,000
18,500,000
900,000

TC Test: MPN/100mL
Rep. No. 1
Rep. No. 2
1011.2
1011.2
1011.2
1011.2
960
689.3
3
5.2
1011.2
1011.2
360.9
344.1
8.7
6.3

E. Coli test: MPN/100mL

Rep. No. 1 Rep. No. 2
1011.2
1011.2
1011.2
1011.2
360.9
416
2
1
1011.2
1011.2
113
113
5.2
4.1

---

---

<1

<1

<1

<1

1,000

2,000

<1

<1

<1

<1

6,440
5,100

6,000
6,100

<1
<1

<1
<1

<1
<1

<1
<1

10

<10

<1

<1

<1

<1

<1

<1

<1

<1

<1

<1

<1

<1