Repeat#for#Q2# with#different# forceps#and#the# corresponding# solution.

Sterilize#lab# surface,#wash# hands,#put#on# apron#and# goggles.#

Choose#3# products#(plus# control)#and# assign#a# number#to#each# product.#

Label#prepared# petri#dish# sections,#add# initials#and# class#period.#

Teacher#adds# liquid#E.#coli.#

Place#lid#on# petri#dish#and# swirl#so#the# liquid#covers# the#agar#(1G5# minutes).#

Use#forceps#to# pick#up#a#single# sterile#disk.#

For#the#control# quadrant,#dip# disk#in#distilled# water#and#place# in#the#middle#of# the#Q1# quadrant.#

Repeat#for#Q3# with#different# forceps#and#the# corresponding# solution.#

Incubate#for#48# hours#at#37# degrees#Celsius.#

Sterilize#tables# and#wash# hands.#

Repeat#for#Q4# with#different# forceps#and#the# corresponding# solution.#

# 48#hours#later# #

Obtain#same#type# of#data#from#Q2.#

Sterilize#lab# surface,#wash# hands,#put#on# apron#and# goggles.#

Examine#petri# dish.#

Measure#zone#of# inhibition#in#3# directions.#

Record#using# ruler#zone#of# inhibition# measurements#in# Data#Table:#Zone# of#Inhibition#for# Q1.#

Obtain#same#type# of#data#from#Q3.#

If#diameters#are# uneven,#calculate# the#average.#

Sterilize#tables# and#wash#hands.#

Obtain#same#type# of#data#from#Q4.#