SETTING UP OF IUI LAB

Dr. Suryakant Hayatnagarkar

Cryo Cell India Pvt. Ltd.

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Rationale for doing I.U.I

Optimization of gamete availability at the site of fertilization

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Reduction in Distance traveled by Sperm

Tackling of occult Seminal problems Optimization of ovulation & Endometrium

Ideal Goals of Sperm Processing

Select highest number of motile sperms Remove Seminal Plasma & Prostaglandins Remove dead , damaged & abnormal sperms

Remove white cells & round cells

Induce Capacitation & Hyperactivation

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IUI is an effective,non invasive,

relatively simple & inexpensive

method of treatment. It can be provided easily in simple setups.

Careful selection & counseling of

couples is essential.
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SETTING UP IUI LAB

Essential requisites of setting up ART services Good lab design Good service delivery
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SETTING UP IUI LAB

Place

Equipment
Personnel Facilities
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PLACE

One room preferably 100 sq feet Air conditioned Away from busy areas, dust Preferably in basement Near OT or insemination room
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SECTIONS IN IUI LABORATORY

Semen

analysis and processing laboratory Semen sample collection cabin (Masturbatory) IUI procedures room
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DESIGNING OF LAB

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OTHER ANCILLARY AREAS

Reception

and waiting area Subfertility clinic Male clinic/ Andrology clinic Doctors cabin Transvaginal Ultrasonography room
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TESTING EQUIPMENT Binocular Microscope OR Trinocular microscope

Microscope should have camera port and 20x objective Phase contrast accessory would be ideal for semen analysis laboratory Cryo Cell

TESTING EQUIPMENT
SPERM COUNTING CHAMBER
Neubaurs Chamber Cryocell sperm counting chamber Makler chamber

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PROCESSING EQUIPMENT CENTRIFUGE

Swing

out rotor RPM meter Timer Cooling/ auto breaks not essential
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PROCESSING EQUIPMENT
INCUBATOR TEST TUBE WARMER
Digital

indicator and PLC controller desirable CO2 incubation not essential for IUI work.
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ANCILLARY EQUIPMENT

Laminar Flow Bench

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ANCILLARY EQUIPMENT

Refrigerator
For storage of media and kits
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ACCESSORIES FOR PROCESSING

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Media Used In Sperm Processing

Use media manufactured by reputed manufacturer, do not use from cottage manufacturer or fly-by-night operators Do not use media in open package, or bulk pckings as it is likely to be contaminated before finishing. Insist on quality certification / check for manufacturers instructions before using.

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Media Used In Sperm Processing

HAM F-10 EBSS HUMAN TUBAL FLUID MEDIUM Additives Pentoxyphyline Albumin

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Advantages of Density Gradient Centrifugation

1. Can be used for both normal and oligospermic samples 2. Concentrates highly motile sperm in the pellet. 3. Effectively removes seminal plasma, round cells and dead sperm 4. Reduces oxidative damage to sperm. 5. Viscous semen samples can be processed. 6. Enhances percentage of spermatozoa with normal morphology.
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PERSONNEL

Trained Gynecologist Trained Technician Other support services

Assistants Sonologist

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WHAT CRYOCELL CAN DO FOR YOU Supply Quality reagents and Kits Help in designing IUI lab and selecting equipments for the lab Help in procuring the equipment
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WHAT CRYOCELL CAN DO FOR YOU

Train

Gynecologists in procedures Train Technicians in processing techniques Supply tested Quarantined Frozen Donor Semen samples Troubleshooting
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