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    Kazi Sumon
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    The document contains 4 questions regarding preparing solutions from stock concentrations for molecular biology experiments. 1) To make a 1X TBE solution in 10L requires 1L of 10XTBE concentrate and 9L of water. 2) To obtain 1ug of DNA from a 500ug/mL stock requires 3uL of the stock DNA solution and 999,997uL of water. 3) To make a 0.1ug/uL plasmid solution in 600uL requires 120uL of a 0.5ug/uL plasmid stock and 480uL of water. 4) To make solutions of 50nM TAE buffer, 25mM EDTA, and 1

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    The document contains 4 questions regarding preparing solutions from stock concentrations for molecular biology experiments. 1) To make a 1X TBE solution in 10L requires 1L of 10XTBE concentrate and 9L of water. 2) To obtain 1ug of DNA from a 500ug/mL stock requires 3uL of the stock DNA solution and 999,997uL of water. 3) To make a 0.1ug/uL plasmid solution in 600uL requires 120uL of a 0.5ug/uL plasmid stock and 480uL of water. 4) To make solutions of 50nM TAE buffer, 25mM EDTA, and 1

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    3 views2 pages

    MolBio Assigment

    Uploaded by

    Kazi Sumon
    The document contains 4 questions regarding preparing solutions from stock concentrations for molecular biology experiments. 1) To make a 1X TBE solution in 10L requires 1L of 10XTBE concentrate and 9L of water. 2) To obtain 1ug of DNA from a 500ug/mL stock requires 3uL of the stock DNA solution and 999,997uL of water. 3) To make a 0.1ug/uL plasmid solution in 600uL requires 120uL of a 0.5ug/uL plasmid stock and 480uL of water. 4) To make solutions of 50nM TAE buffer, 25mM EDTA, and 1

    Copyright:

    Attribution Non-Commercial (BY-NC)
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    of 2

    Kazi Sumon Dr.

    Esther Peterson Molecular Biology Lab Assignment #2

    1. What volume of 10XTBE do you need to make a 1X TBE solution in a final volume of 10L using water as diluent? C1V1=C2V2 10(V1) = 1(10L) V1 = 10L/10 = 1 liter of the 10XTBE and 9L of water 2. How many microliters do you need to get a total of 1ug of DNA if you have a stock concentration of 500ug/mL of DNA? How much of water do you need? 500ug/ml (X mL) = (1ug/mL) (1mL) X ml = 1ug/(500ug/mL) = .003ml = 3 uL of stock. 1000000ul-3ul= 999,997ul of water 3. How many microliters of 0.5ug/ul of plasmid do you need to get 0.1ug/ul solution in a final volume of 600ul using water? How much of water (ul) do you need? C1V1=C2V2 0.5ug/ul (V) = 0.1ug/ul (600ul) V = [0.1ug/ul (600ul)] / 0.5ug/ul V = 120ul of plasmid 600ul-120ul= 480 ul of water 4. If you have the following ingredients 0.5M TAE buffer, 0.25M EDTA and 100% mercaptoethanol, how much of each do you need to make a solution 50nM of TAE buffer, 25mM of EDTA and 1% of - mercaptoethanol in a final volume of 50ML using water as the diluent? How much of water do you need?

    To make 50nM of TAE buffer


    Stock: .5M = .5 moles / Liter = 5 x 10^8nM

    C1V1=C2V2 (5 x 10^8 nM) (V1) = (50 nM) (1L) V1 = (50 nano-moles) / (5 x 10^8 nM) = 1 x 10^-7 Liter of stock TAE buffer and (1Liter-.0000001L=) .9999999L of water.

    To make 25mM of EDTA


    Stock: 0.25M EDTA = 250mM C1V1=C2V2 (250mM) (V1) = (25mM) (1L) [(25mM)(1L)] / 250mM = 0.1 Liter of stock and (1.0 L - 0.1 L=) .9L of water

    To make 1% of - mercaptoethanol in a final volume of 50ML


    Stock: 100% of - mercaptoethanol = 1g/ml .01g/ml (50ml) = .5g needed in 50ml 1g/ml (Xml) = .5g .5g / (1g/ml) = .5ml of 100% stock 50ml-.5ml= 45.5ml of water

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