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Micro propagation (tissue culture or in-vitro culture) refers to the multiplication of plants, in aseptic condition and in artificial growth medium. From very small plant parts like meristem tip, callus embryos, anther etc. the German plant physiologist Heberland in1902 first described the biological principles of tissue and organ culture. After that rapid advantages have been
made in plant tissue culture. At present tissue culture finds extensive application in horticulture in several countries. Though some achievements have been made, commercial utilization of tissue culture is still not to the satisfaction in all the major crops .
6. Micro propagation facilitates long distance transport of propagation material and long term storage of clonal materials. 7. Tissue culture methods are not viable (male sterile) or not available easily (e.g. banana ) and in plant where propagation by conventional methods are expensive ( e.g. orchid ).
12-03-2013