Scribd Logo
Upload

Welcome to Scribd!

  • Immediate Spin: AHG Phase

    Uploaded by

    Dennis Valdez
    5 views1 page
    The AHG phase is the last phase of testing serum and red blood cells for compatibility, usually in test tubes. During this phase, anti-human globulin is added to the serum-red blood cell mix after incubation and washing at 37C. The anti-human globulin causes agglutination of any red blood cells coated with IgG antibodies, indicating the presence of an incompatible antibody through an indirect antiglobulin test. Most clinically significant antibodies will be detected at the AHG phase.

    Original Description:

    .

    Original Title

    AB ID

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    The AHG phase is the last phase of testing serum and red blood cells for compatibility, usually in test tubes. During this phase, anti-human globulin is added to the serum-red blood cell mix after incubation and washing at 37C. The anti-human globulin causes agglutination of any red blood cells coated with IgG antibodies, indicating the presence of an incompatible antibody through an indirect antiglobulin test. Most clinically significant antibodies will be detected at the AHG phase.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    5 views1 page

    Immediate Spin: AHG Phase

    Uploaded by

    Dennis Valdez
    The AHG phase is the last phase of testing serum and red blood cells for compatibility, usually in test tubes. During this phase, anti-human globulin is added to the serum-red blood cell mix after incubation and washing at 37C. The anti-human globulin causes agglutination of any red blood cells coated with IgG antibodies, indicating the presence of an incompatible antibody through an indirect antiglobulin test. Most clinically significant antibodies will be detected at the AHG phase.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    AHG Phase The last of the so-called "phases" of testing serum and red cells for compatibility, most

    commonly in test tubes (the first two are the immediate spin and the 37C phases). In classic tube testing, the AHG phase occurs after the serum and red cells have undergone an incubation at 37C followed by a washing step. During this phase, anti-human globulin (yes, that's where "AHG" comes from) is added to the serum-red cell mix, and it agglutinates red cells that are coated with IgG antibody. This process is simply an indirect antiglobulin (Coombs) test, and it shows that there is an antibody present that is incompatible with the red cells present in the test tube. Most clinically significant antibodies will react at the AHG phase. - See more at: http://www.bbguy.org/education/glossary/index.aspx?alphabet=A&id=191#sthash.pAYkks0Z.dpuf

    Blaney, K. D., & Howard, P. R. (2000).Basic & applied concepts of immunohematology. St. Louis: Mosby.

    You might also like