ByAssma Parveen Roll no.

11-AMJ-01 Deptt of ABT

Gene structure -Promoter -Terminator

Promoter

5UTR

CDS

3UTR

Terminator

Genomic DNA

transcription mRNA
translation

protein
4

Promoter determines:
1. 2. 3. 4.

Which strand will serve as a template. Transcription starting point. Strength of polymerase binding. Frequency of polymerase binding.

 One

type of RNA polymerase. Pribnow box located at 10 (6-7bp) 35 sequence located at -35 (6bp)

Conclusions:
1. 2.

Promoters are very hard to predict. Promoter prediction must be organismdependent (and even polymerasedependent).

 At

the end of the coding region beyond the terminator codon or codons,there are certain sequences from the TER codon which provide a sequence for the transcript to generate a secondary structure that facililates the termination of transcription.
A disassociation signal at the end of the gene that stops elongating and releases RNA polymerase. terminators (eukaryotes and prokaryotes) form a secondary structure.

All