Rutgers University, Department of Chemical and Biochemical Engineering, Department of Biomedical Engineering.

CONVENT ON!" # CRO$CO%& NOTE$

Vie'ing a $pecimen (Transmitted)
*+ #ove reflective slider to left until at last of three positions+ ,+ Rotate condenser to either position -D C +./*+0- or -D C +*/+1- corresponding to the numerical aperture of the selected o23ective+ 1+ Rotate top polari4er out of light path+ 0+ Clic5 on -T- under Conv in Control %anel 'indo'+ .+ Turn on halogen light source ne6t to microscope and ad3ust intensity+ 7+ 8ocus on sample+ 9+ !lign the condenser+ (see transmitted light notes)

Vie'ing a $pecimen ($ingle 8luorescence)

*+ #ove reflective slider to left to either middle position or rightmost position+ (middle position for red emission : rightmost for green emission) ,+ Clic5 on -8"- under Conv in Control %anel 'indo'+ 1+ 8ocus on sample+