BI 1 :XC NH C V MU

1.1. XC NH C
1.1.1. NGUYN TC
- Da trn s hp th nh sang ca cc cn l lng trong dung dch.
1.1.2. CCH TIN HNH:
Lp ng chun: o cc c c chun trn my spectrophotometer
- Pha long t dung dch chun o c chun theo bng sau:
STT
V dung dch chun(ml)
V nc ct
c (FTU)
Kt qu o OD
-

1
0
25
0
0

2
0.5
24.5
8
0.031

3
1
24
16
0.078

4
2
23
32
0.138

5
3
22
48
0.273

6
4
21
64
0.339

7
5
20
96
0.429

Biu ng chun gia c (FTU) v hp thu OD:

0.5

y = 0.0048x + 0.0046
R = 0.975

0.45
0.4
hp th OD

0.35
0.3
0.25
0.2
0.15
0.1
0.05
0
0

20

40

60
c (FTU)

80

100

120

hp thu ca mu: o mu nc sui trn my spectrophotometer

Kt qu o OD mu (nc sui) qua 3 ln o ln lt l:0.046;0.047;0.047 => kt
qu trung bnh ca kt qu l 0.047

1.1.3. Tnh ton kt qu

- Da vo phng trnh ng chun c chun ta tnh c ca mu (X)

Y= 0.0048X + 0.0046 X
1.1.4. Nhn xt
- c ca nc ta tnh ton c l 8.833 vt qu gii hn cho php trong nc
ung ng chai l 2 FTU v trong nc cp sinh hot l 5FTU ( theo tiu chun
TCVN 6096:2004 v TCVN 5502 : 2003
- Quy trnh thc hin theo ng thao tc quy trnh phn tch .
1.2. XC NH MU
1.2.1. Nguyn tc:
- Da vo s hp th nh sang ca hp cht mu c trong dung dich
1.2.2. Cch tin hnh
Lp ng chun

V(ml) dung dch mu 0

0.5
1
chun A
V(ml) dung dich 25 24.5 24
H2SO4
mu (Pt-Co)
0
10
20
hp th OD
0
0.053 0.058
hp th OD ca mu (nc cp) l 0.050

23

21

19

17

40
80
120
160
0.064 0.073 0.083 0.098

0.12

y = 0.0004x + 0.0352
R = 0.6865

hp th OD

0.1
0.08
0.06
0.04
0.02
0
0

20

40

60

80
100
mu (Pt-Co)

1.2.3. Tnh ton kt qu

- Da vo ng chun ta tnh c mu ca nc l
y = 0.0004x + 0.0352

120

140

160

180

1.2.4. Nhn xt
- mu ta tnh ton c trong nc cp l 37 (Pt-Co) vt qu gii hn cho php
trong nc cp l 15 (Pt-Co) (theo TCVN TCVN 5502 : 2003)
- Quy trnh thc hin theo ng thao tc quy trnh phn tch .