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Journal Reading

Endoscopic Jejunal Biopsy Culture : a Simple and Effective Method


to Study Jejunal Microflora
Chandra Sharad, Dutta Usha, Noor M. Talha, Taneja Neelam, Kochlar Rakesh, et al
Indian J Gastroenterol, Januar !
th
"#$$
%acteriolo&ical studies o' uncontaminated u((er jejunal 'luid )ere (er'ormed
in *+ normal su,jects -+./ sterile0. The main &rou(s o' microor&anisms isolated
)ere Stre(tococcus s( -12iridans &rou(10, 3acto,acillus s(., 2eillonella (ar4ula,
5ctinomces s(., 6aemo(hilus s(., Corne,acterium s(., and Candida al,icans, each
'ound in more than $#/ o' the su,jects. 7nl the Stre(tococcus s(. and
entero,acteria )ere 'ound in +/ o' the su,jects, The num,er o' su,jects har,ourin&
,acteria and the distri,ution o' ,acterial s(ecies )ere the same in ,oth se8es and in
di''erent a&e &rou(s. Jejunal 'luid culture is the &old standard 'or assessin& jejunal
micro'lora. 5s the microor&anisms rests on the mucosal sur'ace, culture o' the
mucosal ,io(s ma ,e a (ossi,le alternati4e method.
To stud the role o' jejunal mucosal ,io(s culture to assess jejunal
micro'lora and to com(are it )ith jejunal 'luid culture.
Thirt adult su,jects )ith &astroeso(ha&eal re'lu8 disease re9uirin& endosco(
under)ent enterosco(. Jejunal 'luid as(irate and mucosal ,io(s )ere cultured. The
(rocedure )as re(eated a'ter ome(ra:ole thera( in $* (atients.
;rom <* (airs -.# (reome(ra:ole thera( and $* (ostome(ra:ole thera(0 o'
'luid and mucosal ,io(sies )ere cultured. In <+ o' the <* (airs -=</0, ,oth the
culture o' jejunal ,io(s and jejunal 'luid ielded similar results )ith res(ect to the
(resence -n>"?0 or a,sence o' &ro)th -n>$*0. In the remainin& . (airs, the &ro)th
)as (resent either in the ,io(s culture -n>"0 or in the 'luid culture -n>$0 onl.
5mon& those (airs in )hich &ro)th )as (resent, microor&anisms isolated )ere
identical in +./, di''ered , " or&anism in .?/ and di''erent , @" or&anisms in
$#/. Ten o' the $" (atients )ho )ere detected to ha4e small intestinal ,acterial
o4er&ro)th -SI%70 on 'luid culture )ere also detected to ha4e SI%7 on ,io(s
culture. Sensiti4it, s(eci'icit, (ositi4e and ne&ati4e (redicti4e 4alue o' ,io(s
culture in dia&nosin& SI%7 )as *..+/, =?."/, =<.?/, and =$.!/, res(ecti4el.
Conclusion o' this stud is culture o' un)ashed endosco(ic jejunal mucosal
,io(s is an e''ecti4e and sim(le alternati4e to jejunal 'luid culture 'or assessin&
jejunal micro'lora.

Will be presented by Junaidi
On Saturday,
nd
September !"

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