1) The study compared culture of endoscopic jejunal mucosal biopsies to culture of jejunal fluid in assessing jejunal microflora in 30 subjects with gastroesophageal reflux disease.
2) Both methods yielded similar results in 28 of 30 pairs of biopsies and fluid cultures. In the remaining 2 pairs, growth was present in either the biopsy or fluid culture alone.
3) The conclusion is that culture of unwashed endoscopic jejunal mucosal biopsies is an effective and simple alternative to jejunal fluid culture for assessing jejunal microflora.
1) The study compared culture of endoscopic jejunal mucosal biopsies to culture of jejunal fluid in assessing jejunal microflora in 30 subjects with gastroesophageal reflux disease.
2) Both methods yielded similar results in 28 of 30 pairs of biopsies and fluid cultures. In the remaining 2 pairs, growth was present in either the biopsy or fluid culture alone.
3) The conclusion is that culture of unwashed endoscopic jejunal mucosal biopsies is an effective and simple alternative to jejunal fluid culture for assessing jejunal microflora.
1) The study compared culture of endoscopic jejunal mucosal biopsies to culture of jejunal fluid in assessing jejunal microflora in 30 subjects with gastroesophageal reflux disease.
2) Both methods yielded similar results in 28 of 30 pairs of biopsies and fluid cultures. In the remaining 2 pairs, growth was present in either the biopsy or fluid culture alone.
3) The conclusion is that culture of unwashed endoscopic jejunal mucosal biopsies is an effective and simple alternative to jejunal fluid culture for assessing jejunal microflora.
Endoscopic Jejunal Biopsy Culture : a Simple and Effective Method
to Study Jejunal Microflora Chandra Sharad, Dutta Usha, Noor M. Talha, Taneja Neelam, Kochlar Rakesh, et al Indian J Gastroenterol, Januar ! th "#$$ %acteriolo&ical studies o' uncontaminated u((er jejunal 'luid )ere (er'ormed in *+ normal su,jects -+./ sterile0. The main &rou(s o' microor&anisms isolated )ere Stre(tococcus s( -12iridans &rou(10, 3acto,acillus s(., 2eillonella (ar4ula, 5ctinomces s(., 6aemo(hilus s(., Corne,acterium s(., and Candida al,icans, each 'ound in more than $#/ o' the su,jects. 7nl the Stre(tococcus s(. and entero,acteria )ere 'ound in +/ o' the su,jects, The num,er o' su,jects har,ourin& ,acteria and the distri,ution o' ,acterial s(ecies )ere the same in ,oth se8es and in di''erent a&e &rou(s. Jejunal 'luid culture is the &old standard 'or assessin& jejunal micro'lora. 5s the microor&anisms rests on the mucosal sur'ace, culture o' the mucosal ,io(s ma ,e a (ossi,le alternati4e method. To stud the role o' jejunal mucosal ,io(s culture to assess jejunal micro'lora and to com(are it )ith jejunal 'luid culture. Thirt adult su,jects )ith &astroeso(ha&eal re'lu8 disease re9uirin& endosco( under)ent enterosco(. Jejunal 'luid as(irate and mucosal ,io(s )ere cultured. The (rocedure )as re(eated a'ter ome(ra:ole thera( in $* (atients. ;rom <* (airs -.# (reome(ra:ole thera( and $* (ostome(ra:ole thera(0 o' 'luid and mucosal ,io(sies )ere cultured. In <+ o' the <* (airs -=</0, ,oth the culture o' jejunal ,io(s and jejunal 'luid ielded similar results )ith res(ect to the (resence -n>"?0 or a,sence o' &ro)th -n>$*0. In the remainin& . (airs, the &ro)th )as (resent either in the ,io(s culture -n>"0 or in the 'luid culture -n>$0 onl. 5mon& those (airs in )hich &ro)th )as (resent, microor&anisms isolated )ere identical in +./, di''ered , " or&anism in .?/ and di''erent , @" or&anisms in $#/. Ten o' the $" (atients )ho )ere detected to ha4e small intestinal ,acterial o4er&ro)th -SI%70 on 'luid culture )ere also detected to ha4e SI%7 on ,io(s culture. Sensiti4it, s(eci'icit, (ositi4e and ne&ati4e (redicti4e 4alue o' ,io(s culture in dia&nosin& SI%7 )as *..+/, =?."/, =<.?/, and =$.!/, res(ecti4el. Conclusion o' this stud is culture o' un)ashed endosco(ic jejunal mucosal ,io(s is an e''ecti4e and sim(le alternati4e to jejunal 'luid culture 'or assessin& jejunal micro'lora.
Will be presented by Junaidi On Saturday, nd September !"