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Detergent Handbook
Detergent Handbook
Detergents
A Handbook & Selection
Guide to Detergents &
Detergent Removal
Hydrophobic tail
Polar Head
Figure 1: The structure of the detergent SDS (Sodium Dodecyl
Sulfate).
Polar head
Hydrophobic
tail
Phospholipid
Lipid Bilayer
Figure 3: The structure of a lipid bilyar and a phospholipid.
Membrane
Detergent
Low CMC
High CMC
Protein-Detergent Complexes
100
80
60
Critical Micelle
Concentration
40
20
Detergent Concentration
Lipid-Detergent Micelles
Detergent Micelles
Figure 5: Schematic showing the stages of protein
solubilization with detergent.
Description
Size
DGA01
Optimizer blueBALLS
500
Non-ionic Detergents
Triton X-100
Octylphenolpoly(ethyleneglycolether)x
Description
DG007
5 x 10ml vials
Size
DG008
10 x 10ml vials
DG507
50ml bottle
DG517
100ml bottle
DG907
5 x 10ml vials
DG908
10 x 10ml vials
786-513
500ml
786-514
1L
Triton X-114
Brij 58
Description
DG009
5 x 10ml vials
Size
DG010
10 x 10ml vials
DG509
50ml bottle
DG518
100ml bottle
786-515
500ml
786-516
Description
DG005
5 x 10ml vials
Size
DG006
10 x 10ml vials
DG505
50ml bottle
DG516
100ml bottle
786-352
250gm
786-522
500gm
1L
Tween 20
Brij 35
Description
DG003
5 x 10ml vials
Size
DG004
10 x 10ml vials
DG503
50ml bottle
DG515
100ml bottle
786-351
250gm
786-521
500gm
Description
DG011
5 x 10ml vials
Size
DG012
10 x 10ml vials
DG511
50ml bottle
DG519
100ml bottle
786-517
500ml
786-518
1L
Tween 80
Octyl Glucoside
N-Octyl-beta-D-glucopyranoside
Description
DG013
5 x 10ml vials
Size
DG014
10 x 10ml vials
DG513
50ml bottle
DG520
100ml bottle
786-519
500ml
786-520
1L
Description
Size
DG015
Octyl Glucoside
1gm
DG016
Octyl Glucoside
5gm
MEGA 8
Octanoyl-N-methylglucamide
Nonylphenyl-polyethylene glycol
Cat.#
Description
DG001
5 x 10ml vials
DG002
10 x 10ml vials
Cat.#
Description
Size
DG501
50ml bottle
DG017
MEGA 8
1gm
DG514
100ml bottle
DG018
MEGA 8
5gm
DG901
5 x 10ml vials
DG902
10 x 10ml vials
786-511
500ml
786-512
1L
MEGA 9
BigCHAP
Nonanoyl-N-methylglucamide
N,N-Bis[3-(D-gluconamido)propyl]cholamide
Description
Size
DG019
MEGA 9
1gm
DG020
MEGA 9
5gm
MEGA 10
Description
Size
DG023
BigCHAP
1gm
DG024
Decanoyl-N-methylglucamide
BigCHAP
5gm
N,N-Bis[3-(D-gluconamido)propyl]deoxycholamide
Type: Non-ionic detergent
Molecular Formula: C17H35NO6
Molecular Weight: 349.5
Form: White powder
Purity: >99%
Solubility: Water soluble
Critical Micelle Conc. (CMC): 6-7mM (25C)
Application: Non-ionic detergent that is water
soluble and readily removed by dialysis. Used for
the solubilization of membranes.
Available as Research Grade detergents.
Cat.#
Description
Size
DG021
MEGA 10
1gm
DG022
MEGA 10
5gm
Description
Size
DG025
1gm
DG026
5gm
2D-Detergent
Ultra Low Conductivity & Low Carbonyl
& Peroxide Contaminants
Carbonyl Contamination
500
20
Commercial
G-Biosciences
[Peroxide]
450
80
[Carbonyl]
72
[Peroxide]
12
300
48
250
10
250
40
200
200
32
150
24
100
16
50
80
Brij 35
Peroxide Contamination
70
G-Biosciences
Commercial
[Peroxide] (M)
56
300
[Carbonyl] (M)
350
50
[Carbonyl] (M)
[Carbonyl] (M)
14
100
200
400
150
400
16
350
600
400
500
18
[Carbonyl]
450
800
64
[Peroxide] (M)
1000
Features
Low conductivity; <15S
Low peroxide contamination
Low carbonyl contamination
Reduced metal ions
Ready to use 10% aqueous solutions
Sealed under inert gas to prevent oxidation.
G-Biosciences
Commercial
Description
DG007
5 x 10ml vials
DG008
10 x 10ml vials
DG009
5 x 10ml vials
DG010
10 x 10ml vials
30
DG003
5 x 10ml vials
20
DG004
10 x 10ml vials
10
DG005
5 x 10ml vials
DG006
10 x 10ml vials
Commercial
G-Biosciences
[Peroxide] (M)
60
50
40
Size
DG011
5 x 10ml vials
DG012
10 x 10ml vials
DG013
5 x 10ml vials
DG014
10 x 10ml vials
Features
Low peroxide contamination
Low carbonyl contamination
Low conductivity
Reduced metal ions
Ready to use 10% aqueous solutions
Sealed under inert gas to prevent oxidation
We offer a selection of widely used Proteomic
Grade Detergent Solutions. The aldehyde and
peroxide levels are <100M and <50M respectively
with a conductivity of <50S.
DG001
5 x 10ml vials
DG002
10 x 10ml vials
DG907
5 x 10ml vials
DG908
10 x 10ml vials
DG901
5 x 10ml vials
DG902
10 x 10ml vials
Brij 35
ionic Detergents
Deoxycholate
Description
DG090
100gm
Size
DG091
500gm
SDS
Description
DG092
SDS
100gm
DG093
SDS
500gm
100ml
R014
Size
CTAB
Hexadecyltrimethylammonium bromide
Description
Size
DG094
CTAB
25gm
DG095
CTAB
100gm
Zwitterionic Detergents
CHAPSO
3-[(3-Cholamidopropyl)dimethylammonio]-2hydroxy-1-propanesulfonate
CHAPS
3-[(3-Cholamidopropyl)dimethylammonio]-1propanesulfonate
Description
Size
DG050
CHAPS
5gm
DG051
CHAPS
25gm
Description
Size
DG052
CHAPSO
1gm
DG053
CHAPSO
5gm
N-Decyl-N,N-dimethyl-3-ammonio-1propanesulfonate
Description
Size
DG054
Sulfobetaine 3-10
1gm
DG055
Sulfobetaine 3-10
5gm
ASB-14
N-Dodecyl-N,N-dimethyl-3-ammonio-1propanesulfonate
Amidosulfobetaine-14
Cat.#
Description
Size
DG056
Sulfobetaine 3-12
1gm
Cat.#
Description
Size
DG057
Sulfobetaine 3-12
5gm
DG060
ASB-14
1gm
DG061
ASB-14
5gm
N-Tetradecyl-N,N-dimethyl-3-ammonio-1propanesulfonate
ASB-16
Amidosulfobetaine-16
Cat.#
Description
Size
DG058
Sulfobetaine 3-14
1gm
DG059
Sulfobetaine 3-14
5gm
Cat.#
Description
Size
DG062
ASB-16
1gm
DG063
ASB-16
5gm
ASB-C8
4-n-Octylbenzoylamido-propyl-dimethylammonio
sulfobetaine
Description
Size
DG064
ASB-C8
1gm
DG065
ASB-C8
5gm
Non-Detergent Sulfobetaine
NDSB 201
3-(1-Pyridino)-1-propane sulfonate
DetergentOUT SDS-300
Specifically developed for the removal of SDS
and supplied with a SDS detection and estimation
kit to determine efficiency of SDS removal.
DetergentOUT DTG-100X
For the removal of a wide variety of detergents,
including Triton X-100, Nonidet P-40, Tween
20, CTAB, CHAPS, and deoxycholate from protein
solutions.
Both DetergentOUT kits are offered in two sizes:
Micro Kit for up to 0.5ml protein solution/ column
Medi Kit for up to 2ml protein solution/ column
2
Description
Size
DG080
NDSB 201
25gm
DG081
NDSB 201
100gm
Protein (mg/ml)
1.8
Detergent (%)
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Before
After
SDS
Before
After
Triton X-100
REFERENCES
1.8
Detergent (%)
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Before
After
SDS
Before
After
Triton X-100
Description
Size
786-150
10
786-151
10
786-152
10
786-153
10
786-127
For 10ml
786-128
For 30ml
OrgoSol DetergentOUT
Suitable for hydrophobic proteins,
removes detergents and concentrates
protein solutions
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0
4 5 6 7
[SDS] (g)
9 10
Description
SDS Detection & Estimation Reagent Kit
Size
15 assays
Detergent
Type
Cat #
DG023
1gm
DG024
5gm
DG025
1gm
DG026
Molecular
Weight
Size
5gm
CMC1
(mM)
Aggr.
No.2
HLB3
Average
Micellar
Weight
Cloud
Point
(C)
Conductivity
(S)
Research Grade
Deoxy
BigCHAP
Research Grade
Research Grade
10 x 10ml
DG503
DG515
10 x 10ml
DG505
1gm
DG018
5gm
DG019
1gm
DG020
5gm
DG021
1gm
DG022
5gm
786-511
Research Grade
MEGA 9
Research Grade
MEGA 10
Research Grade
Research Grade
Proteomic Grade
(10% aqueous
solution)
786-512
2D-Detergent
(10% aqueous
solution)
Research Grade
Research Grade
DG002
10 x 10ml
DG501
Proteomic Grade
(10% aqueous
solution)
50ml
DG901
Research Grade
DG015
1gm
DG016
5gm
786-513
24-40
16.9
48,000
>100
<50
1122
0.0070.077
70
15.7
79,000
>100
321.4
58
335.4
19-25
349.5
6-7
573
0.050.3
45-50
<50
<50
1 liter
10 x 10ml
DG507
50ml
<15
292.4
20-25
84
25,000
>100
5 x 10ml
DG008
13.5
80,000
65
500ml
786-516
100155
10 x 10ml
786-515
0.20.9
5 x 10ml
DG908
647
1 liter
<50
100ml
DG907
5 x 10ml
DG010
10 x 10ml
DG509
100ml
786-517
<15
50ml
500ml
786-518
5 x 10ml
DG012
10 x 10ml
DG511
50ml
DG519
100ml
537
0.35
12.4
23
<50
1 liter
DG011
Proteomic Grade
(10% aqueous
solution)
0.09
500ml
786-514
DG518
Research Grade
627
10 x 10ml
DG009
Proteomic Grade
(10% aqueous
solution)
5 x 10ml
DG902
DG517
2D-Detergent
(10% aqueous
solution)
Tween 20
100ml
DG007
Triton X-114
10,500
5 x 10ml
DG514
Triton X-100
1 liter
DG001
Octyl
Glucoside
8-16
500ml
Proteomic Grade
(10% aqueous
solution)
MEGA 8
Nonidet P-40
Substitute
1.11.4
100ml
DG017
862.1
50ml
DG516
<25 in 10%
solution
5 x 10ml
DG006
500gm
DG005
8,800
100ml
786-522
-4
50ml
Research Grade
Brij 58
DG004
10
5 x 10ml
Proteomic Grade
(10% aqueous
solution)
3.4
500gm
DG003
Brij 35
786-521
878.1
1227
0.06
16.7
76
<50
Detergent
Type
Research Grade
Cat #
Size
786-519
DG013
Proteomic Grade
(10% aqueous
solution)
Aggr.
No.2
5 x 10ml
DG014
10 x 10ml
DG513
Cloud
Point
(C)
100ml
25gm
DG095
100gm
DG090
100gm
DG091
500gm
DG092
100gm
DG093
500gm
R014
100ml
DG060
1gm
DG061
5gm
DG062
1gm
DG063
5gm
DG064
1gm
DG065
5gm
Conductivity
(S)
-
50ml
DG094
HLB3
Average
Micellar
Weight
1 liter
DG520
Tween 80
CMC1
(mM)
500ml
786-520
Molecular
Weight
1325
0.012
60
15
76,000
65
<50
IONIC DETERGENTS
CTAB
Proteomic Grade
Deoxycholate
Proteomic Grade
SDS
Proteomic Grade
10% Solution
364.5
170
62,000
414.6
4-8
3-12
16
1,2005,000
288.38
7-10
62
40
18,000
>100
ZWITTERIONIC DETERGENTS
ASB-14
Proteomic Grade
ASB-16
Proteomic Grade
ASB-C8
Proteomic Grade
CHAPS
Proteomic Grade
CHAPSO
Proteomic Grade
Sulfobetaine
3-10
Proteomic Grade
Sulfobetaine
3-12
Proteomic Grade
Sulfobetaine
3-14
Proteomic Grade
NDSB 201
Proteomic Grade
DG050
5gm
DG051
25gm
DG052
1gm
DG053
5gm
DG054
1gm
DG055
5gm
DG056
1gm
DG057
5gm
DG058
1gm
DG059
5gm
DG080
25gm
DG081
100gm
434.7
<50 in 10%
solution
462.7
<50 in 10%
solution
440.6
614.9
6-10
10
6,150
>100
<25 in 10%
solution
630.9
11
7,000
90
<50 in 10%
solution
307.5
25-40
41
12,600
335.5
2-4
55
18,500
364
0.10.4
83
30,200
NON-DETERGENT SULFOBETAINE
201.4
Critical Micellular Concentration (CMC) determined at 20-25C. CMC is the concentration at which
micelles begin to form.
2
Aggregation number is the average number of monomers in a micelle.
3
Hydrophile-Lipophile Balance (HLB) defines the hydrophilic character of a detergent.
4
Data not available
Triton is a registered trademark of Union Carbide Corp; Tween is a registered trademark of Uniqema, a
business unit of ICI Americas, Inc.; Nonidet is a registered trademark of Shell Chemicals; Brij is a registered
trademark of ICI Americas, Inc.
1
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