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  • Standard Adition Vit C. Harris

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    Joana Ferreira
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    This document describes a standard addition experiment to determine the concentration of ascorbic acid in a sample. Ascorbic acid solutions of increasing concentration were added to a 50 mL sample, and the resulting current was measured. A calibration curve was generated by plotting current versus ascorbic acid concentration, from which the concentration of ascorbic acid in the original sample could be determined. The slope and intercept of the calibration curve were used to calculate this concentration as 0.114 mM, equivalent to 1.00 g of ascorbic acid per tablet.

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    This document describes a standard addition experiment to determine the concentration of ascorbic acid in a sample. Ascorbic acid solutions of increasing concentration were added to a 50 mL sample, and the resulting current was measured. A calibration curve was generated by plotting current versus ascorbic acid concentration, from which the concentration of ascorbic acid in the original sample could be determined. The slope and intercept of the calibration curve were used to calculate this concentration as 0.114 mM, equivalent to 1.00 g of ascorbic acid per tablet.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
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    3 views2 pages

    Standard Adition Vit C. Harris

    Uploaded by

    Joana Ferreira
    This document describes a standard addition experiment to determine the concentration of ascorbic acid in a sample. Ascorbic acid solutions of increasing concentration were added to a 50 mL sample, and the resulting current was measured. A calibration curve was generated by plotting current versus ascorbic acid concentration, from which the concentration of ascorbic acid in the original sample could be determined. The slope and intercept of the calibration curve were used to calculate this concentration as 0.114 mM, equivalent to 1.00 g of ascorbic acid per tablet.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    Vitamin C Standard addition experiment

    Add 100 mM ascorbic acid to 50 mL sample


    Vs=
    mL ascorbic acid added

    Vo(mL)
    51
    [S]i (mM)

    0
    0.5
    1.25
    2.25

    100

    I(s+x)=
    signal (uA)
    9.236
    14.5
    19.3
    24.88

    x-axis
    Si*Vs/(Vo+Vs)
    0.000
    0.971
    2.392
    4.225

    y-axis
    I(s+x)*V/Vo
    9.236
    14.64215686
    19.77303922
    25.97764706

    30

    y = 3.8713x + 10.063
    R = 0.9886

    25

    I, mA

    20
    15
    10
    5

    -2.500

    -1.500

    0
    -0.500

    0.500

    1.500

    2.500

    3.500

    [Acorbic Acid], mM

    Equao da reta
    Slope
    Intercept
    Y=0 (mM)

    3.871
    10.06
    2.599

    Factor diluio (x)


    Conc AA (mM)
    AA por comprimido (g)

    51
    0.133
    1.17

    4.500

    5.500

    Vitamin C Standard addition experiment


    Add 100 mM ascorbic acid to 50 mL sample
    Vs=
    mL ascorbic acid added

    Vo(mL)
    51
    [S]i (mM)

    0
    0.5
    1.25

    100

    I(s+x)=
    signal (uA)
    9.236
    14.5
    19.3

    x-axis
    Si*Vs/(Vo+Vs)
    0.000
    0.971
    2.392

    y-axis
    I(s+x)*V/Vo
    9.236
    14.64215686
    19.77303922

    25

    20

    I, mA

    y = 4.3459x + 9.6784
    R = 0.9848

    15

    10

    -2.500

    -1.500

    -0.500

    0.500

    1.500

    [Acorbic Acid], mM

    Equao da reta
    Slope
    Intercept
    Y=0 (mM)
    Factor diluio (x)
    Conc AA (mM)
    AA por comprimido (g)

    4.3459
    9.678
    2.227
    51
    0.114
    1.00

    2.500

    3.500

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