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  • Keto Acid Decarboxylation Lineweaver-Burk Plot

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    Anonymous 75XlKJ7erc
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    This document contains data from experiments measuring the initial velocity of keto acid decarboxylation at various substrate concentrations. Lineweaver-Burk, Eadie-Hofstee, and Hanes plots were constructed from the initial velocity data and used to determine the kinetic parameters Km and Vmax. Additional experiments were conducted with varying concentrations of one substrate (A) while holding the concentration of the other substrate (B) constant. First and second degree plots were constructed from this data to determine the kinetic mechanism and interaction between substrates.

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    probset sept 26.xlsx

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    This document contains data from experiments measuring the initial velocity of keto acid decarboxylation at various substrate concentrations. Lineweaver-Burk, Eadie-Hofstee, and Hanes plots were constructed from the initial velocity data and used to determine the kinetic parameters Km and Vmax. Additional experiments were conducted with varying concentrations of one substrate (A) while holding the concentration of the other substrate (B) constant. First and second degree plots were constructed from this data to determine the kinetic mechanism and interaction between substrates.

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    5 views7 pages

    Keto Acid Decarboxylation Lineweaver-Burk Plot

    Uploaded by

    Anonymous 75XlKJ7erc
    This document contains data from experiments measuring the initial velocity of keto acid decarboxylation at various substrate concentrations. Lineweaver-Burk, Eadie-Hofstee, and Hanes plots were constructed from the initial velocity data and used to determine the kinetic parameters Km and Vmax. Additional experiments were conducted with varying concentrations of one substrate (A) while holding the concentration of the other substrate (B) constant. First and second degree plots were constructed from this data to determine the kinetic mechanism and interaction between substrates.

    Copyright:

    © All Rights Reserved
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    of 7

    Keto acid concentration (M)

    Initial velocity (umol CO2/min)


    2.500
    1.000
    0.714
    0.526
    10.00
    0.250

    0.294

    Keto acid 0.250


    decarboxylation
    0.209
    Lineweaver-Burk
    Plot

    8.00

    Lineweaver-Burk
    1/v

    f(x) = 1.2345655436x + 2.9345217813


    R = 0.994341311

    1/[S]6.00
    3.40
    1/v
    4.00
    4.80
    5.41
    7.81

    0.185
    0.128

    0.40
    1.00
    1.40
    1.90
    4.00

    4.00
    2.00
    -

    0.50

    1.00

    1.50

    2.00

    2.50

    3.00

    3.50

    1/[S]

    b=1/(Vmax)
    Vmax=1/b
    Vmax=1/2.934
    Vmax
    m=(km/Vmax)
    km=Vmax*m
    km

    0.341

    0.421

    Eadie-Hofstee
    v/[S]

    v
    0.12
    0.25
    0.29
    0.35
    0.51

    Keto acid 0.294


    decarboxylation
    Eadie-Hofstee
    Plot
    0.250
    0.50
    0.40

    v/[S]

    m=-(1/km)
    km=-(1/m)
    km
    b=(Vmax)/km
    Vmax=km*b
    Vmax
    Hanes Plot

    0.209
    0.185
    0.128

    0.60

    f(x) = - 2.2594313724x + 0.7861503921


    R = 0.9775566709

    0.30
    0.20
    0.10

    0.443

    0.100

    0.150

    0.200

    0.250
    v

    0.348

    0.300

    [S]/v

    [S]
    8.50
    4.00
    3.42
    2.84
    1.95

    Keto acid 2.500


    decarboxylation
    1.000
    Hanes
    Plot
    0.714
    0.526
    0.250

    10.00
    8.00

    f(x) = 2.8870639145x + 1.2635562205


    R = 0.9985459527

    6.00
    [S]/v

    m=1/(Vmax)
    Vmax=1/m
    Vmax
    b=km/(Vmax)
    km=Vmax*b
    km

    4.00
    2.00
    -

    0.500

    1.000
    0.346

    1.500
    [S]

    0.437

    2.000

    2.500

    arboxylation
    r-Burk Plot

    2.9345217813

    .00

    2.50

    3.00

    3.50

    4.00

    4.50

    1/[S]

    arboxylation
    stee Plot

    x + 0.7861503921

    0.250
    v

    0.300

    0.350

    arboxylation
    Plot

    1.2635562205

    1.500
    [S]

    2.000

    2.500

    3.000

    v (katal/kg) [A] (uM)


    0.095
    22
    0.112
    30
    0.141
    50
    0.196
    200
    0.102
    22
    0.120
    30
    0.155
    50
    0.223
    200
    0.112
    22
    0.136
    30
    0.180
    50
    0.284
    200
    0.125
    22
    0.156
    30
    0.218
    50
    0.385
    200
    Second Degree Plot
    1/[B]
    Slope
    0.050
    134.4
    0.040
    132.5
    0.025
    133.8
    0.010
    133.8

    Varying [A] and Constant [B]


    [B] (uM)
    1/[A]
    1/v
    0.045
    10.53
    0.033
    8.93
    20
    0.020
    7.09
    0.005
    5.10
    12.00
    0.045
    9.80
    10.00
    0.033
    8.33
    25
    0.020
    6.45
    8.00
    0.005
    4.48
    6.00
    0.045
    8.93
    1/v
    4.00
    0.033
    7.35
    40
    0.020
    5.56
    2.00
    0.005
    3.52
    0.00
    0.045
    8.00
    0.0000.0050.0100.0150.0200.0250.03
    0.033
    6.41
    100
    0.020
    4.59
    1/[A]
    0.005
    2.60

    First Degr
    Varying [A], C

    First Degree Plot


    arying [A], Constant [B]
    [B]=20 uM
    [B]=25 uM
    [B]=40 uM
    [B]=100 uM

    0100.0150.0200.0250.0300.0350.0400.0450.050
    1/[A]

    Equations:
    at [B]=20 uM
    at [B]=30 uM
    at [B]=40 uM
    at [B]=100 uM

    R^2
    y=134.4x
    y=132.5x
    y=133.8x
    y=133.8x

    +
    +
    +
    +

    4.423
    3.829
    2.866
    1.925

    0.999
    0.999
    0.999
    0.999

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