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  • Data Uji Antioksidan

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    Eunike Prananingtyas
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    This document summarizes the results of an antioxidant assay of extracts and fractions from an unknown sample. Absorbance measurements were taken at 5 concentrations of the extract and fractions. Linear regression analysis was used to determine the antioxidant activity, expressed as IC50 values, of the extract and fractions. The average IC50 values were -2.244 mg/ml for the extract and 14.316 mg/ml for the fractions. In conclusion, the extract showed higher antioxidant activity than the fractions based on the lower IC50 value.

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    Data Uji antioksidan

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    This document summarizes the results of an antioxidant assay of extracts and fractions from an unknown sample. Absorbance measurements were taken at 5 concentrations of the extract and fractions. Linear regression analysis was used to determine the antioxidant activity, expressed as IC50 values, of the extract and fractions. The average IC50 values were -2.244 mg/ml for the extract and 14.316 mg/ml for the fractions. In conclusion, the extract showed higher antioxidant activity than the fractions based on the lower IC50 value.

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    © All Rights Reserved
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    4 views7 pages

    Data Uji Antioksidan

    Uploaded by

    Eunike Prananingtyas
    This document summarizes the results of an antioxidant assay of extracts and fractions from an unknown sample. Absorbance measurements were taken at 5 concentrations of the extract and fractions. Linear regression analysis was used to determine the antioxidant activity, expressed as IC50 values, of the extract and fractions. The average IC50 values were -2.244 mg/ml for the extract and 14.316 mg/ml for the fractions. In conclusion, the extract showed higher antioxidant activity than the fractions based on the lower IC50 value.

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    © All Rights Reserved
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    of 7

    Data Uji Antioksidan Ekstrak dan Fraksi

    Konsentrasi Abs ekstrak Abs fraksi Abs


    seri (mg/ml) kontrol
    R1 R2 R3 R1 R2 R3
    0,04 0,267 0,266 0,265 0,150 0,149 0,149

    0,08 0,073 0,074 0,073 0,116 0,116 0,116

    0,16 0,053 0,053 0,053 0,128 0,128 0,128 0,607

    0,32 0,121 0,121 0,121 0,086 0,086 0,086

    0,4 0,161 0,162 0,162 0,169 0,169 0,169

    A. EKSTRAK
    %S (R1)
    a. (0,607-0,267)/0,607 x 100% = 56,013%
    b. (0,607-0,073)/0,607 x 100% = 87,974%
    c. (0,607-0,053)/0,607 x 100% = 91,269%
    d. (0,607-0,121)/0,607 x 100% = 80,066%
    e. (0,607-0,161)/0,607 x 100% = 73,476%

    KURVA EKSTRAK R1
    90

    85

    80

    75

    70

    65
    0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45

    Regresi linier

    A = 75,398

    B = 11,806

    R = 0,131
    Mencari IC50

    Y = bx + a

    50 = 11,806x + 75,398

    X = -2,151

    SD dari konsentrasi vs abs = 0,155

    CV = 0,155/0,2 x 100 = 77,5%

    %S (R2)

    a. (0,607-0,266)/0,607 x 100% = 56,178%


    b. (0,607-0,074)/0,607 x 100% = 87,809%
    c. (0,607-0,053)/0,607 x 100% = 91,269%
    d. (0,607-0,121)/0,607 x 100% = 80,066%
    e. (0,607-0,162)/0,607 x 100% = 73,311%

    KURVA EKSTRAK R2
    90

    85

    80

    75

    70

    65
    0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45

    Regresi linier

    A = 75,448

    B = 11,394

    R = 0,127

    Mencari IC 50
    Y = bx + a

    50 = 11,394x + 75,448

    X = -2,233

    SD dari konsentrasi vs abs = 0,155

    CV = 0,155/0,2 x 100 = 77,5%

    %S (R3)

    a. (0,607-0,265)/0,607 x 100% = 56,343%


    b. (0,607-0,073)/0,607 x 100% = 87,974%
    c. (0,607-0,053)/0,607 x 100% = 91,269%
    d. (0,607-0,121)/0,607 x 100% = 80,066%
    e. (0,607-0,162)/0,607 x 100% = 73,311%

    KURVA EKSTRAK R3
    90

    85

    80

    75

    70

    65
    0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45

    Regresi linier

    A= 75,610

    B= 10,913

    R= 0,122

    Mencari IC50
    Y = bx + a

    50 = 10,913x + 75,610

    X = -2,347

    SD dari konsentrasi vs abs = 0,155

    CV = 0,155/0,2 x 100 = 77,5%

    Rata-rata IC50 ekstrak = -2,244

    B. FRAKSI
    %S (R1)
    a. (0,607-0,150)/0,607 x 100% = 75,288%
    b. (0,607-0,116)/0,607 x 100% = 80,890%
    c. (0,607-0,128)/0,607 x 100% = 78,913%
    d. (0,607-0,086)/0,607 x 100% = 85,832%
    e. (0,607-0,169)/0,607 x 100% = 72,158%

    KURVA FRAKSI R1
    90

    85

    80

    75

    70

    65
    0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45

    Regresi Linier

    A = 78,987

    B = -1,854
    R = - 0,055

    Mencari IC50

    Y = bx + a

    50 = -1,854x + 78,987

    X = 15,635

    SD dari konsentrasi vs abs = 0,155

    CV = 0,155/0,2 x 100 = 77,5%

    %S (R2)
    a. (0,607-0,149)/0,607 x 100% = 75,453%
    b. (0,607-0,116)/0,607 x 100% = 80,890%
    c. (0,607-0,128)/0,607 x 100% = 78,913%
    d. (0,607-0,086)/0,607 x 100% = 85,832%
    e. (0,607-0,169)/0,607 x 100% = 72,158%

    KURVA FRAKSI R2
    90

    85

    80

    75

    70

    65
    0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45

    Regresi linier
    A = 79,075
    B =-2,129
    R =-0,063
    Mencari IC50
    Y= bx + a
    50= -2,129x + 79.075
    X =13,657

    SD dari konsentrasi vs abs = 0,155

    CV = 0,155/0,2 x 100 = 77,5%

    %S (R3)
    a. (0,607-0,149)/0,607 x 100% = 75,453%
    b. (0,607-0,116)/0,607 x 100% = 80,890%
    c. (0,607-0,128)/0,607 x 100% = 78,913%
    d. (0,607-0,086)/0,607 x 100% = 85,832%
    e. (0,607-0,169)/0,607 x 100% = 72,158%

    KURVA FRAKSI R3
    90

    85

    80

    75

    70

    65
    0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45

    Regresi linier
    A = 79,075
    B =-2,129
    R =-0,063
    Mencari IC50
    Y = bx + a
    50 = -2,129x + 79.075
    X =13,657
    SD dari konsentrasi vs abs = 0,155
    CV = 0,155/02 x 100 = 77,5%

    Rata-rata IC50 = 14,316

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