DNA SCIENCE AND BIOTECHNOLOGY QUIZ REVIEW

QUIZ DATE: FRIDAY, DECEMBER 12, 2014

The following questions cover the material weve covered from November 10 up until December 10. Completing
the questions alone will help you review the content that will be tested, but you should also be reviewing all the
notes, the lab, and assignments completed. The test will consist of a mix of multiple choice and short answer
questions.
Biotechnology Introduction (Notes)
What are the three general areas of biotechnology?
Recombinant DNA (Notes, Textbook, and WebQuest)
Scientists
What did Luria and Bertani observe in the bacteria they studied?
What special protein did Arber Linn, Meselson, and Yuan first observe?
Briefly describe the work of Smith and Wilcox.
What was the significance of Nathans experiment?
Compare the experiments done by Berg to that of Boyer, Cohen and Chang. How are they similar? How
are they different?
Concepts

What is a restriction enzyme?

What does restriction activity mean?
What does modification activity mean?
What is EcoRI?
What is pSC101?
Outline the creation of recombinant DNA using the method used by Boyer, Cohen and Chang.
What is the significance of DNA ligase?
What is meant by transformation?

Escherichia coli and Plasmid Vectors (WebQuest)

What makes E. coli a good host organism?

What are some of the important advances in biotechnology, agriculture, or medicine that can be
attributed to the use of E. coli?
What are plasmids?
How are plasmids used in recombinant technology?
Antimicrobial Resistance (Article assignment, Video Summary, Class Discussion)

Describe the Antibiotics Crisis in relation to evolutionary cause and effect, and the widespread
use of antibiotics for the last 70 years.
What does this growing antibiotic resistance mean for the future development of antibiotics and
how we treat illness and disease?
Isolating Colonies Laboratory

Why is it important to obtain individual colonies of bacteria that are genetically homogenous?
How does the streaking techniques used help achieve this?
What is meant by rendering cells more competent?
Why do you need to flame the inoculating loop before each streak?
Describe the difference between bacteriostats and bacteriocides.
How does ampicillin work?
How does kanamycin work?
How does pAMP and the ampicillin resistance gene work?